Absolute Antibody - Engineered recombinant antibodies for all
Absolute Antibody, ISO-certified and now part of Absolute Biotech, has the vision to make recombinant antibody technology accessible to all. They are specialized in antibody sequencing, antibody engineering and recombinant expression and are producing an ever-increasing number of engineered recombinant antibodies. Absolute Antibody offers recombinant production as custom services, as well as a unique catalog of recombinant antibodies and Fc Fusion proteins, engineered into new and useful formats.
Advantages of recombinant antibodies
- Ensured batch-to-batch reproducibility
- High purity, low endotoxin
- Defined – no variations, no contamination, no genetic drift, no accidental loss
- Animal-free manufacturing
- Possibility of endless reproduction
- Possibility to engineer new formats
- Switching of species, isotypes / subtypes
- Chimerization
- Humanization
- Antibody fragments
- Bi- and tri-specifics
- Fc Silent™ versions etc.
Non-IgG isotypes
In addition to all IgG subtypes, Absolute Antibody offers antibodies in the following isotypes: IgM, IgA, IgE, IgD and IgY.
Chimeric Antibodies
Absolute Antibody offers an antibody chimerization service for human, mouse, rat, rabbit and hamster IgG with other species and isotypes available on request.
Antibody fragments
Absolute Antibody converts any antibody into a fragment, including single chain Fv (scFv), Fab or Fab2 formats. Ideal full-length alternative in certain situations!
Fc-Silent versions
Fc Silent™ is a Fc domain developed at Absolute Antibody with abrogated binding of Fc receptors, abolishing antibody directed cytotoxicity effector function.
VivopureX™ recombinant mouse antibodies - Ideal for in vivo research
VivopureX™ antibodies are recombinant mouse antibodies ideal for in vivo research in mouse models.
The collection consists of popular antibody clones, many originally obtained from rats or hamsters, which Absolute Antibody has engineered into mouse-anti-mouse recombinant versions to improve research results. These species-matched chimeric antibodies consist of a clone’s original antigen-binding variable domain with a mouse constant domain. Read more
Advantages of VivopureX™ recombinant mouse antibodies
- Reduced immunogenicity: no neutralizing antibodies are induced in the host organism, meaning the antibody works for longer and cohorts respond more consistently.
- Tailored effector function: antibody formats have been engineered to suit their most popular application. (Increased Fc receptor binding increases depleting potential, while reduced Fc receptor binding reduces side effects in blocking.)
- Increased potency: less antibody is required to achieve the same response in mouse models.
- Batch-to-batch reproducibility: recombinant production ensures the highest possible reproducibility, and guarantees high purity and low endotoxins (<0.5 EU/mg).
Supporting data for VivopureX™ antibody: PD-1 mouse IgG2a Fc Silent™ antibody [RMP1-14], Ab00813-2.3
Mean tumor sizes in animals treated with PD-1 mouse IgG2a Fc Silent™ antibody based on clone RMP1-14 (Ab00813-2.3) are significantly smaller than in animals treated with the traditional rat IgG2a antibody (Ab00813-7.1)
The data illustrate how VivopureX™ mouse antibodies provide increased long-term efficacy, potency and consistency compared to the original rat antibodies. Recombinant mouse PD-1 antibody based on the widely used clone RMP1-14 reduced tumor size in a mouse model more effectively than the original rat version.
Download research poster on the engineered PD-1 antibody to learn more
Looking for another example? One customer used a species-matched mouse-anti-mouse antibody to deplete CD8+ T-cells in mice more completely and for longer than the original rat antibody. Read the full case study here.
VivopureX™ antibody collection includes a selection of the most exciting mouse-anti-mouse antibodies
| Antibody Name [Clone] | Engineered Format | Top Application |
||
|---|---|---|---|---|
| Anti-OX40/CD134 [OX86] |
Order |
Mouse IgG1 |
Agonism |
|
| Anti-OX40/CD134 [OX86] | Order |
Mouse IgG2a | Agonism | |
| Anti-PD-1 [RMP1-14] |
Order |
Mouse IgG2a Fc Silent™ |
Blocking |
|
| Anti-CTLA-4 [9D9] |
Order |
Mouse IgG2a | Blocking, Treg depletion | |
| Anti-CTLA-4 [9D9] | Order |
Mouse IgG2a Fc Silent™ | Blocking, peripheral Treg expansion | |
| Anti-GITR [DTA-1] |
Order |
Mouse IgG2a |
Agonism |
|
| Anti-CD25 [PC-61.5.3] |
Order |
Mouse IgG2a |
Depletion |
|
| Anti-TIGIT [1B4] |
Order |
Mouse IgG1 |
Blocking |
|
| Anti-Lag3 [C9B7W] | Order |
Mouse IgG2a Fc Silent™ | Blocking |
|
| Anti-CD96 [6A6] |
Order |
Mouse IgG2a Fc Silent™ |
Blocking |
|
| Anti-IL-10R [1B1.3a] |
Order |
Mouse IgG2a Fc Silent™ |
Blocking |
|
| Anti-BTLA [HMBT-6B2] |
Order |
Mouse IgG2a Fc Silent™ |
Blocking | |
| Anti-Tim-4 [3A1] |
Order |
Mouse IgG2a Fc Silent™ |
Blocking | |
| Anti-Tim-4 [5G3] | Order |
Mouse IgG2a Fc Silent™ |
Blocking | |
| Anti-TIM-2 [RMT2-14] | Order |
Mouse IgG2a Fc Silent™ |
Blocking | |
| Anti-Tim-1 [3B3] | Order |
Mouse IgG1 | Agonism | |
| Anti-Tim-3 [2C12] | Order |
Mouse IgG2a Fc Silent™ |
Blocking | |
| Anti-CD115/M-CSFR [AFS98] | Order |
Mouse IgG2a | Depletion | |
| Anti-PD-L1 [10F.9G2] | Order |
Mouse IgG2b |
Blocking | |
| Anti-PD-L1 [10F.9G2] | Order |
Mouse IgG2b Fc Silent™ |
Blocking | |
| Anti-IFN-gamma receptor 1 [GR20] | Order |
Mouse IgG2b Fc Silent™ |
Blocking | |
| Anti-VISTA [13F3] | Order |
Mouse IgG2b Fc Silent™ |
Blocking | |
| Anti-CD20 [18B12] | Order |
Mouse IgG2a | Depletion | |
| Anti-CD4 epitope A [YTS 177.9] | Order |
Mouse IgG2a | Depletion | |
| Anti-CD4 epitope A [YTS 191.1] | Order |
Mouse IgG2a | Depletion | |
| Anti-CD8 alpha/Lyt-2 [YTS 169.4] | Order |
Mouse IgG2a | Depletion | |
| Anti-Ly6G [1A8] | Order |
Mouse IgG2a | Depletion | |
| Anti-CD200R [OX131] | Order |
Mouse IgG1 Fc Silent™ |
Blocking | |
| Anti-PD-L2 [TY25] | Order |
Mouse IgG2a Fc Silent™ |
Blocking | |
| Anti-Ly6G/Ly6C [RB6-8C5] | Order |
Mouse IgG2a | Depletion | |
| Anti-Galectin 9 [RG9-35] | Order |
Mouse IgG2a Fc Silent™ | Blocking | |
| Anti-CD153 [RM153] | Order |
Mouse IgG2a | Blocking | |
| Anti-CD153 [RM153] | Order |
Mouse IgG2a Fc Silent™ | Blocking | |
| Anti-CD155 [3F1] | Order |
Mouse IgG2a Fc Silent™ | Blocking | |
| Anti-CD226 [10E5] | Order |
Mouse IgG2a Fc Silent™ | Blocking | |
| Anti-CD45R [RA3-6B2] | Order |
Mouse IgG2a | Depletion | |
| Anti-CD19 [6D5] | Order |
Mouse IgG2a | Depletion | |
| Anti-HVEM [HMHV-1B18] | Order |
Mouse IgG2a Fc Silent™ | Blocking | |
| Anti-CD27 [RM27-3E5] | Order |
Mouse IgG1 | Agonism | |
| Anti-CD22 [OX-97] | Order |
Mouse IgG2a Fc Silent™ | Blocking | |
| Anti-CD47 [mIAP301] | Order |
Mouse IgG2b Fc Silent™ | Blocking |
Custom Services offered by Absolute Antibody
In addition to the comprehensive catalog of reagents for both diagnostic and research purposes, Absolute Antibody provides custom services and thereby specializes in the sequencing, engineering, and recombinant manufacture of antibodies. Secure, adjust or improve your own antibodies! Engineer or have produced your specific antibody!
Antibody Sequencing
Sequence from hybridoma cells or from purified antibody protein. Sequence your antibody to enable recombinant production or to obtain intellectual property rights for your sequence!
Hybridoma Sequencing
Starting point for protecting your antibody and embarking on antibody engineering projects such as species, isotype and subtype switching or even humanization.
Antibody Protein Sequencing
If the cell line has been lost or is otherwise unavailable, but sufficient protein is still available, advances in mass spectrometry technology have made it possible to sequence the protein.
Polyclonal Antibody Sequencing
Immortalization service to secure a precisely defined and reproducible source of your antibodies.
Antibody Engineering
Rapidly reformat your antibody into any species or isotype. Services include humanization and custom bispecifics.
Absolute Antibody has developed a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Their antibody engineering services include chimerization, humanization, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies.
Other Species
Currently Absolute Antibody has antibody sequences available for a large number of animals ranging from pig, sheep, horse, dog, chicken, fish, platypus and a whole range of non-human primates. Moreover, their experts are able to determine antibody sequences from most genomes (even if only partial assemblies are available).
Chimerization
Absolute Antibody offers an antibody chimerization service which enables you to create a chimeric antibody, consisting of the original variable domains from your antibody with the constant domains from a different species, for almost any mammalian species.
See also chimeric antibodies
Humanization
Absolute Antibody offers a royalty-free antibody humanization service. Absolute Antibody’s Prometheus™ antibody humanization service delivers a panel of humanized variants from your clone, guaranteeing to produce a variant with comparable activity to the parent antibody.
See also antibody humanization
Isotype switching
Absolute Antibody’s isotype switching service (also known as class switching) allows you to alter the isotype or subtype of any antibody. This can be used to alter the in vivo effector function and stability of an antibody, to overcome e.g. aggregation properties or to convert your IgG to an IgM for increased avidity.
Antibody fragments
In certain situations antibody fragments offer an ideal alternative to full-length antibodies. Absolute Antibody’s proprietary recombinant expression and antibody engineering platform enables to convert any antibody into a fragment, such as a single chain Fv (scFv), Fab or Fab2, followed by expression and purification at any scale.
See also antibody fragments page
Bispecific antibodies
Absolute Antibody offers a bispecific antibody service. They can reformat any pair of antibodies into an IgG-scFv format (i.e. an IgG with a scFv fused to the C-terminus of the IgG). This results in a bispecific bivalent antibody format capable of binding two antigens simultaneously.
Engineered Fc domains
Absolute Antibody can engineer the Fc domain of your antibody to mediate effector function or extend the plasma half-life. For example, they can abolish Fc domain binding to FcγR using our Fc Silent™ mutation or mAbsolve’s STR mutations, as well as extend or shorten the plasma half-life of your IgG antibody by introducing mutations in the Fc domain.
...and more
The Periodic Table of Antibodies shown below gives you an impression possible antibody formats .
Antibody Expression
Produce your antibody in Absolute Antibody’s recombinant expression system at milligram-to-gram scale.
Absolute Antibody’s antibody expression platforms rapidly produces high-quality, animal-free recombinant antibodies at milligram-to-gram scale in HEK or CHO cells. Their serum-free mammalian transient expression system allows to manufacture recombinant antibodies in any species, isotype or format with high purity and low endotoxin levels. You can start from an electronic antibody sequence, hybridoma cell line or purified antibody protein.
All production occurs at ISO 9001:2015-certified manufacturing facility.
All antibody expression services are royalty-free and customizable to your needs.
HEXpress™ HEK Antibody Production
HEXpress™ service produces milligram-to-gram quantities of recombinant antibodies in HEK cells with high purity and low endotoxin levels. This service provides a faster, more affordable alternative to stable cell line generation, with proven expression of antibodies in any species, isotype or format. Read more
CHXpress™ CHO Antibody Production
CHXpress™ service enables serum-free mammalian transient expression in CHO cells to better serve clients through their full antibody development. This service is particularly useful for bridging the gap to stable CHO production, or for tackling challenging constructs. Read more
FleXpress™ High-Throughput Antibody Production
FleXpress™ service allows to rapidly express large numbers of IgG antibodies at 80 ml scale using the proven recombinant antibody technology. Particularly useful for early-stage pilot studies on a group of therapeutic antibody candidates, as well as converting a monoclonal antibody portfolio from hybridomas to recombinant production. Read more
Recombinant F4/80 clone CI:A3-1
Uniquely available at Absolute Antibody!
Anti-F4/80 [Cl:A3-1] antibody is the most widely published and referenced mouse macrophage and microglia marker. At Absolute Antibody, they have sequenced the original hybridoma (Gordon and Austyn, 1981) and produced recombinant and chimeric anti-F4/80 clone Cl:A3-1 versions, allowing greater experimental flexibility and control for the end user!
Absoulte Antibody is the ONLY commercial manufacturer …
- … of recombinant F4/80 [Cl:A3-1] monoclonal antibody
- … of recombinant chimeric mouse anti-mouse F4/80 [CI:A3-1] monoclonal antibody
- … of recombinant rat anti-mouse and chimeric mouse anti-mouse F4/80 [CI:A3-1] antibodies with an effector-silent Fc domain (Fc Silent™).
Supporting Data for Anti-F4/80 [CI:A3-1 (recombinant version)]
In Figure 1 murine bone marrow-derived macrophages (BMDMs) were pre-blocked with rat anti-mouse CD16 & CD32 (clone FCR-4G8) and stained with non-recombinant anti-F4/80 [Cl:A3-1] conjugated to Alexa Fluor® 647 (AF647), all commercially available from competitors. In Figure 2 BMDMs were stained with recombinant anti-F4/80 [Cl:A3-1] or isotype control (anti-fluorescein [4-4-20 (enhanced)] IgG2b (Ab00102-8.1). In Figure 3 BMDMs were stained with Fc Silent™ recombinant anti-F4/80 [Cl:A3-1] (Ab00106-8.4) or isotype control (Fc Silent™ anti-fluorescein [4-4-20 (enhanced)] IgG2b (Ab00102-8.4). These were fluorescently labelled using the secondary antibody, goat IgG anti-rat IgG (H&L-chain) polyclonal antibody directly conjugated to Alexa Fluor® 647(AF647) commercially available from a competitor. Whilst in Figure 2 the highest fluorescence signal is seen with the recombinant anti-F4/80 IgG2 (the isotype of the original hybridoma-derived antibody), the isotype control IgG2b (Ab00102-8.1) shows considerable signal overlap, indicative of binding of the antibody to Fc-receptors. This illustrates the importance of isotype controls in such experiments when using conventional antibody formats particularly when Fc-blocking reagents are incompatible with the system used due to reactivity with the secondary antibody. The Fc silent™ format however overcomes this issue as seen in Figure 3, where the Fc silent™ recombinant anti-F4/80 (Ab00102-8.4) yields a strong and distinct signal, whilst the isotype control (Ab00102-8.4) shows no discernible difference to the background staining from the secondary antibody alone. Therefore, with Fc Silent™ reagents, no Fc-blocking products are required. [Data courtesy of Lewis Taylor.]
