Bio-Helix Promotion

Buy 5 Get 1 Free

Valid on the entire Bio-Helix product range. Ordering a total of 6 units, one of which is free of charge. Expires 31st Dec 2022. Not summable with other discounts.

Bio-Helix Free Sample: Western ECL Substrate

Use the chance to order a free sample of Bio-Helix most sensitive
UltraScence Femto Plus Western ECL Substrate.

Offer valid while supply lasts.

Bio-Helix: Protein Detection Products

Bio-Helix offers a range of high quality protein characterisation tools including Protein Ladders for SDS-PAGE, electrophoresis stains such as Coomassie blue or Ponceau S as well as ECL Luminescence Substrates for protein detection in Western Blots.

COOMASSIE Nano Protein Staining Solution

  • Ready-to-use protein staining solution for SDS-PAGE gels
  • Fast protein detection (5 min.)
  • No risk of overstaining (e.g. overnight incubation)
  • High sensitivity down to 10ng
  • No need for destaining
  • Washing step can be omitted
  • Safe and environmentally friendly (no methanol, no acetic acid)
  • Compatible with mass spectrophotometry

Formats: 1x conc. 500ml | 5x conc. 1L (= 5L)

Fast staining within 5 Min.

COOMASSIE Nano Protein Staining Solution visualizes protein bands already within the first 5 minutes of staining. Prestained (M) and unstained protein ladder (U), and BSA were applied in electrophoresis.

High Sensitivity

COOMASSIE Nano Protein Staining Solution detects proteins with high sensitivity down to 10 ng (Lane H).

Benchmark of COOMASSIE Nano with other brands:
Equivalent or even superior performance

COOMASSIE Nano Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands.

UltraScence Western Substrates, ECL

Bio-Helix UltraScence Pico and Femto Western Substrates are luminol-based enhanced chemiluminescence (ECL) substrates with long signal duration and offer the perfect choice for performing immunoblotting with horseradish peroxidase (HRP)-conjugated secondary antibodies. Read more

  • No protocol change when switching from other brands to UltraScence
  • 2-year storage at room temperature without affecting performance
  • Different sensitivities, optimized for different protein amounts available
  • Enhanced chemiluminescence signal duration
  • Optimized for use with PVDF and Nitrocellulose membranes.
  • Compatible with Western Blotting Markers.
  • Optimized for film and CCD-based imaging

UltraScence Pico Plus Western Substrate

low picogram or high femtogram detection

UltraScence Pico Ultra Western Substrate

low picogram or mid femtogram detection

UltraScence Femto Western Substrate

mid femtogram to low femtogram detection

UltraScence Femto Plus Western Substrate

low femtogram detection (mid-atto) 

Benchmark:
Ultrascence Pico Plus compared to other brands

UltraScence Pico Plus Western Substrate enables an accurate low picogram to high femtogram detection. 

Serial dilutions of EGFP were applied in electrophoresis. After protein transfer, membranes were probed with GFP tag Rabbit PolyAb  followed by Goat Anti-rabbit IgG/HRP secondary antibody. Blots were incubated with UltraScence Pico Plus Western substrate. The blots were and were simultaneously exposed for 5, 10, 30, 60 and 180 seconds.

Benchmark:
Ultrascence Femto and Femto Plus compared to other brands

UltraScence Femto Plus Western Substrate enables an accurate low picogram to high femtogram detection. 

Serial dilutions of EGFP were applied in electrophoresis. After protein transfer, membranes were probed with GFP tag Rabbit PolyAb followed by Goat Anti-rabbit IgG/HRP secondary antibody. Blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375) and were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds and 180 seconds.