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Insulin Assays for Mouse and Rat


Mouse/Rat Insulin (high sensitivity) ELISA

Mouse/Rat Total Insulin
(high sensitivity) ELISA

27707 Mouse/Rat Insulin ELISA

Mouse/Rat Total Insulin
CLEIA

Mouse/Rat Intact Proinsulin ELISA

Mouse/Rat Intact Proinsulin
ELISA

Mouse/Rat Intact Proinsulin CLEIA

Mouse/Rat Intact Proinsulin
CLEIA

 

Introduction 

Measuring rodent insulin in fasting and after feeding is important in diabetic research, however there are some issues to measure such as (1) no much sample amount can be obtained, (2) very low concentration in fasting and (3) huge gap of two concentration of insulin between the samples in fasting and after feeding for measuring in same assay. IBL offers the following 4 assay kits for solving such issues.

 

Advantages

1. Only tiny sample is needed
2. Low conc. in fasting can be measured
3. Low and high conc. in same assay (CLEIA)

 

Product overview

  Sample Type
Cat.No. Product Name Measurement
Range (pg/mL)
Sample Volume Serum EDTA-Plasma Heparin-Plasma Supernatant  
27705 Mouse/Rat Total Insulin (high sensitivity) ELISA 1.25 - 80 2 μL - - Order
27707 Mouse/Rat Total Insulin CLEIA 41.15 - 30,000 5 μL - - Order
27706 Mouse/Rat Intact Proinsulin ELISA 1.4 - 90 20 μL - Order
27708 Mouse/Rat Intact Proinsulin CLEIA 9 – 6,480 10 μL - Order

 

Reference Data

It has been considered that the conversion rate from proinsulin to insulin in type 2 diabetes patients decreases due to dropping down of PC1/3 enzymatic activity by abnormal function of pancreatic β cells.

The ratio of intact proinsulin and insulin “P/I ratio” is known as an index of clinical human pancreatic β-cell function and it is valuable for the evaluation. The ratio can be calculated by the value of Intact proinsulin and total insulin in same assay.

 

Quoted from: The graph is quoted from Anal Biochem. 2015 Mar 15;473:72-9. Comparison data of CLEIA system

 

 

Proinsulin data

Quoted from: The graphs are quoted from Anal Biochem. 2015 Sep 1;484:91-8.

 

References

1. Development of an ultrasensitive immunoassay using affinity maturated antibodies for the measurement of rodent insulin. Imai S et al. Anal Biochem. 2015 Mar 15;473:72-9.
2. Development of a novel immunoassay specific for mouse intact proinsulin. Imai S et al. Anal Biochem. 2015 Sep 1;484:91-8.

 

 

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