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Human Mesenchymal Stem Cell Culture


For differentiation for human MSC's, we offer the MSCgo™ Differentiation Media.

Faster growth rates and stronger cell yields

MSC NutriStem® XF Medium is a defined, serum-free, xeno-free culture medium designed for optimal growth and expansion of human mesenchymal stem/stromal cells (hMSC) derived from a variety of sources, including bone marrow (BM-MSC), adipose tissue (AT-MSC) and umbilical cord matrix (UC-MSC). MSC NutriStem® XF Medium supports long-term growth of hMSC while maintaining their self-renewal and multi-lineage differentiation potential.

hMSCs cultured in MSC NutriStem XF Medium show superior proliferation and self-renewal potential in comparison to serum-containing media and other commercial serum-free media.  In addition, hMSCs maintain their proper fibroblast-like cell morphology, tri-lineage differentiation potential, and demonstrate normal hMSC marker profiles, and karyotypic stability over long-term culture. 

 

Key advantages

  • Serum-free, xeno-free, defined culture system
  • Superior cell growth compared to serum-containing media
  • Supports long-term growth and self-renewal of hMSCs from multiple sources
  • Consistent, reliable MSC cultures
  • Suitable for the harvest of high quality exosomes (EVs) for future clinical applications
  • Eliminates the need to spend time and money pre-qualifying FBS lots
  • Manufactured under cGMP
  • FDA Drug Master File (DMF) available

Cells cultured in serum-containing medium do not require an adaptation phase when transitioning to serum-free MSC NutriStem® XF Medium.

 

hMSC-BM were cultured in MSC NutriStem® XF

Figure: NutriStem® MSC XF Medium promotes superior proliferation and expansion of hMSCs over time as compared to other serum-free and serum-containing media.

 

Sample Data

Initial isolation

Figure: Human mesenchymal stem cells were initially isolated from Wharton's jelly (left image) and placenta (right image) using MSC NutriStem™ XF on pre-coated plates with MSC Attachment Solution (cat. no. 05-752-1). High number of hMSC was obtained.

 

Proliferation

Figure: MSC NutriStem® XF promotes excellent proliferation of hMSC from a variety of sources while maintaining normal fibroblast-like, spindle shape cell morphology.

 

Self-renewal potential

Figure: hMSC-BM and hMSC-AT were expanded in MSC NutriStem® XF for 3-5 passages prior to 14 days of CFU-F assay. Colonies were stained with 0.5% crystal violet (x100). The cells maintain their self-renewal potential.

 

Differentiation potential

Figure: hMSC-BM (top panel) and hMSC-AT (bottom panel) expanded in MSC NutriStem® XF for 3-5 passages prior to trilineage differentiation. Representative images of stained adipocytes (Oil Red O), osteocytes (Alizarin Red) and chondrocytes (Alcian Blue). The cells maintain their multilineage differentiation potential.

 

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T +41 41 420 96 36
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info@lucerna-chem.ch

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Lucerna-Chem AG
Abendweg 18
CH-6006 Luzern

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