Mycoplasma is a prokaryotic microorganism of the class Mollicutes that lack a true cell wall, and many of which are considered pathogenic.
Mycoplasma contamination is often detected in cell cultures, and consequently, virus cultures, vaccines and other biological materials produced in cells become contaminated as well.
Mycoplasma contamination in cell lines used for research poses a serious problem. In most cases, visual detection of such contaminations or detection with the aid of a microscope is impossible. Although mycoplasma does not cause visible damage to cells, it undeniably affects cell metabolism, cell growth in culture, protein synthesis, cytokine secretion, and even causes damage to DNA and RNA. Hence, results obtained from experiments are liable to be biased when mycoplasma is present. Various studies show that the percentage of contaminated cultures in cell banks is 10%-80%. Mycoplasma contamination can originate from bovine serum, laboratory employees, other contaminated cultures, or the animals from which the cells have been harvested.
The most prevalent species of mycoplasma detected in contaminated cell cultures include M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis and M. pirum.
Don't compromise your results! We offer all you need to keep your cell culture Mycoplasma free.
EZ-PCR Mycoplasma Test Kit
Ready-to-use PCR Mix for the detection of mycoplasma in cell culture.
e-Myco™ VALiD-Q Mycoplasma qPCR Detection Kit
Detection of mycoplasmas according to European Pharmacopoeia section 2.6.7.
Pharmacidal Spray and Solution
Mycoplasma elimination reagent.