PEPperCHIP SARS-CoV-2 Proteome Microarray

In light of the ongoing coronavirus disease (COVID-19) pandemic, PEPperPRINT has rapidly developed and produced a new peptide microarray. Derived from the virus isolate sequence under GenBank ID: MN908947.3 (including 49 mutations of the B.1.1.7, 501.V2, B.1.1.28 and P.1 Manaus variants and all spike protein mutations of the Alpha, Beta, Gamma, Delta, Delta Plus and Omicron variants of concern), the PEPperCHIP® SARS-CoV-2 Proteome Microarray gives researchers access to serologically screen 5,347 individual peptides spanning the entire viral proteome.

Identify, profile, and compare specific IgG, IgA, and IgM antibody responses in patient or animal sera for vaccine development, or screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research.

Proposed antibody fingerprinting workflow using the PEPperCHIP® SARS-CoV-2 Proteome MicroarrayThe entire SARS-CoV-2 viral proteome is translated into overlapping peptides and printed onto glass slides. Patient sera is incubated on the chip and antibodies present in the sample bind to epitopes recognized within individual peptides. The resulting response profile may be compared across different samples to monitor B-cell responses over time, or to determine immunodominant epitopes that may be further validated as potential disease-specific biomarkers for IVD development.

Differential IgA and IgG responses to SARS-CoV-2 infection. LEFT: Representative scans of PEPperCHIP®SARS-CoV-2 Proteome Microarray chips incubated with sera collected from infected and non-infected donors. RIGHT: Fluoresence intensity plots showing differential IgA and IgG responses across the SARS-CoV-2 proteome quantified from the microarray scan readouts.