RayBiotech Promotion
50% off Flow Cytometry Antibodies & Kits
RayBright Antibodies, Live/Dead Markers, Intracellular Staining Kits
Offer expires 31st Aug 2023. Not summable with other discounts.
RayBiotech - Flow Cytometry Antibodies & Tools
RayBright dye conjugated Antibodies | Live/Dead Markers | Intracellular Staining Kits
Gain quantitative single cell insights with flow cytometry and FACS reagents that come with the quality expected from RayBiotech. Differentiate your cell populations in complex samples with RayBright conjugated antibodies providing excellent brightness and covering most of the common emission colors of similar dyes!
- RayBiotech offers over 820 Flow Cytometry Antibodies
- RayBiotech offers Live/Dead Markers, Intracellular Staining and Apoptosis Kits
- RayBiotech manufactures in the USA
Flow Cytometry Antibodies
Different cell populations can be distinguished from one another in a complex sample using flow cytometry antibodies conjugated to non-overlapping fluorophores.
RayBiotech offers RayBright® dyes with excellent brightness, covering most of the common emission colors of similar dyes. Read more
RayBright® Dye Labeling Results
| RayBiotech Superior Fluorochromes | Excitation Laser | Max Excitation/Emission | Replacing dyes of equivalent wavelengths |
|---|---|---|---|
| RayBright® UV 450 | UV 355 | 347/448 nm | Alexa Fluor 350 |
| RayBright® Violet 450 | Violet 407 | 403/454 nm | Pacific Blue, Alexa Fluor 450 |
| RayBright® Violet 500 | Violet 407 | 405/537 nm | Alexa Fluor 430, BV510 |
| RayBright® Violet 600 | Violet 407 | 407/600 nm | BD Brilliant Violet 605 |
| RayBright® Violet 655 | Violet 407 | 407/655 nm | BD Brilliant Violet 655 |
| RayBright® Blue 488 | Blue 488 | 491/518 nm | Alexa Fluor 488, FITC, BD BB515 |
| RayBright® Blue/Green 575 | Blue 488/Green 561 | 505/570, 553/570 nm | R-PE, Cy3 |
| RayBright® Green 594 | Green 561 | 594/614 nm | PE-Texas Red, PE-CF594, PE-eFluor610 |
| RayBright® Blue/Green 647 (PE-tandem dye) | Blue 488/Green 561 | 496/664, 565/664 nm | PE-Cy5 |
| RayBright® Blue/Green 780 (PE-tandem dye) | Blue 488/Green 561 | 496/780, 565/780 nm | PE-Cy7, PE-Alexa Fluor 750 |
| RayBright® Red 647 | Red 640 | 649/664 nm | APC, Alexa Fluor 647, Cy5 |
| RayBright® Red 700 | Red 640 | 685/710 nm | Alexa Fluor 700, APC-R700 |
| RayBright® Red 780 | Red 640 | 629/780 nm | Alexa Fluor 750, APC-eFluor 780 |
| Traditional dyes | Excitation Laser | Max Excitation/Emission |
|---|---|---|
| PerCP | Blue 488 | 482/667 nm |
| R-PE | Blue 488/Green 561 | 496/780, 565/780 nm |
| APC | Red 640 | 651/662 nm |
- Hard-to-find violet channel antibodies
- Custom fluorophore conjugation
- Free trial sizes (5 µg) of any of our RayBright® antibodies
- Bulk discounts and long-term supply agreements available
- Custom antibody production available
Detection of specific T helper populations, including Th1, Th2, Th17, and Tregs
Live/Dead Markers - RayBright® LIVE dyes
As understanding of the degree of cell death in a flow cytometry assay is critical towards any analysis of cell population, the use of live/dead markers remain essential to elucidate viability.
RayBright® LIVE dyes are amine-reactive fluorescent dyes that helping distinguish between live and dead cells in flow cytometry analysis. RayBright® LIVE dyes bind with a much brighter signal in dead cells than in living cells. Living cells refuse the dye while dead cells are stained both on the cell surface and intracellularly. Supplied frozen in DMSO solution at a pre-titrated concentration. Read more
Intracellular Staining Kits
The RayBiotech Intracellular Staining Kit is designed to allow for intracellular staining of target cytokines, proteins, or other factors in activated or treated cell populations. The kit includes both Brefeldin A and Monensin both of which inhibit the Golgi apparatus function, trapping normally secreted cytokine and proteins inside cells during incubation. Following incubation, the included saponin-containing buffer is used to permeabilize cells in order to allow intracellular antibodies entry through the plasma membrane. Read more
RayBio® Annexin V Apoptosis Kit (RayBright® V450)
The RayBiotech Annexin V Detection Kit is a sensitive assay of cell apoptosis by flow cytometry. Live and dead cells can be discriminated by RayBright Live/dead markers, a group of small organic dye. Unlike other apoptosis assay using propidium iodide (PI) or 7-AAD, RayBio Annexin V apoptosis Detection Kit can combine with further immunophenotyping that requires cell fixation. Read more
