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Protein A Resin FF Prepacked Column 


Catalog Number Pack Size List Price* Quantity
L00680-12 1ml x 2 293,00 Add
L00680-51 5ml x 1 543,00 Add
L00680-55 5ml x 5 2'244,00 Add

* CHF, excl. 7.7% VAT and shipping costs


Product Description

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens. Characteristics

Category Protein Purification
Supplier GenScript
Storage Conditions Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.
Product Line Research
Tech. Data Sheet View datasheet
Link To Supplier http://www.genscript.com
Shipping Details 4
Material Safety Data Sheet Download PDF
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