6xHis-TEV protease
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
P1001-200-0.2MG | 0.2 mg | 404,00 | Add | |
P1001-1000-1MG | 1 mg | 620,00 | Add | |
P1001-1000-10MG | 10 mg | 2'818,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
Please inquire a quote for two or more UBPBio products
Tobacco Etch Virus (TEV) protease is a cysteine protease that is commonly used to remove fusion tags, including His, GST and MBP, at the N or C termini of recombinant proteins. TEV protease specifically recognizes a seven amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQG/S) and cleaves between Gln and Gly/Ser under a wide range of conditions. Recombinant 6xHis-TEV was expressed in E. Coli and purified to near homogeneity. A Ser219Asn (S219N) substitution was introduced that has no effect on TEV protease activity, but inhibits auto-cleavage and thus, stabilizing TEV protease during incubation and long-term storage.
Category | Protein Purification |
Supplier | UBPBio |
Regulatory Status | RUO |
Species Specificity | Virus |
Format | 100 mM Tris, pH 8.5 at 4 0C, 500 mM NaCl, 5mM DTT, 0.5 mM EDTA and 50% Glycerol |
Storage Conditions | -80°C |
Disclaimer | Shipped on dry ice - extra freight charge will occur |
Tech. Data Sheet | View datasheet |
Link To Supplier | http://www.ubpbio.com |
Shipping Details | Dry ice |
Material Safety Data Sheet | Download PDF |