3D PETG Cell Culture Scaffolds

Copner Biotech focusses in 3D cell culture and associated technologies. Their state of the art design and manufacturing process enables 3D cell culture scaffolds allowing more favourable nutrient and oxygen exchange conditions for cells compared to 2D cell culture. Better representation of physiological conditions in the body provide tangible experimental advantages for research in fields studying cell microenvironments.

Benefits of 3D cell culture

  • Enhancing cell capture and adhesion whilst using low cell seeding concentrations
  • Promoting the growth of balanced, confluent cell systems
  • Physiologically relevant environment, morphologies and more healthy biomarker

Scaffold structure and microstructure

Scaffold microstructure. A discrete oxygen and nutrient gradient across the interface of the 3D PETG scaffold formed by the heterogeneity in pore size, encourages cell proliferation from the centre to the periphery.

Scaffold application examples

Cell growth on 3D PETG scaffold

SEM images of L929 cells grown on PETG scaffold at day 7. Cells demonstrate a successful migration from the centre to the periphery of the scaffold, resulting in a balanced, confluent cell system that resembles the patterns of in vivo tissue.

Spheroid cultures

Spheroid cultures. High cell seeding concentrations and culturing over a prolonged period, promote the formation of spheroid cultures that provide a physicochemical environment similar to that in vivo by facilitating cell-cell and cell-matrix interactions.