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Zymo Environ Water RNA Kit
For SARS-CoV-2 Wastewater Surveillance
 
Simple and affordable Zymo Research RNA kit to detect COVID-19 early in wastewater
 

The most effective wastewater workflow ever

  • Enhanced viral enrichment: Get 8x more viral RNA
  • Pathogen inactivation for safe handling
  • PCR inhibitors removed in one spin
  • ≥ 6 µl elution improves limits of detection
  • RNA ready for RT-qPCR, RT-ddPCR, and NGS
 

Get 8x more SARS-CoV-2 from wastewater for the earliest detection of COVID-19

 
The Zymo Environ Water RNA Kit includes Water Concentrating Buffer (WCB). 5ml of SARS-CoV-2 positive wastewater was processed with and without WCB (n=2). RT-qPCR showed the addition of WCB shifted average Ct values earlier by ~3 (an 8-fold increase in viral recovery).
 

PCR Inhibitor-Free RNA

 
Extracted Wastewater RNA is free of PCR Inhibitors. 50K HeLa cells were spiked into DNA/RNA Shield (positive extraction control) and into wastewater. RNA was extracted (n=2) and RT-qPCR (Quasar 670) analysis showed the ΔCt is <0.5, indicating the extraction system effectively removes inhibitors in wastewater.
 

Safe sample transportation: Water Concentrating Buffer inactivates COVID-19

 

The Water Concentrating Buffer (WBC) included in the Zymo Environ Water RNA Kit inactivates SARS-CoV-2 for safe sample processing, while preserving the integrity of viral RNA.

Viral inactivation demonstrated by plaque formation assay. Vero-E6 cells were infected with serial dilutions of SARS-CoV-2, strain Muc-IMB-1. Virus was applied without pre-treatment, or incubated for 48 hours with agarose overlay before fixation with formaldehyde. Plaques were visualized with crystal violet staining.

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