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First Order Promotion
50% off your 1. SimpleStep Ignite™ ELISA Order
Benefit from 50% discount on your first SimpleStep Ignite™ ELISA order to support your first testing of the new ELISA generation!
Offer valid until 12th August 2026. Not summable with other discounts.
Note: Inquire your discount on the product detail page.
Follow up Promotion
Get 4 for 3 SimpleStep Ignite™ ELISA Kits
For larger evaluation projects: Order 4 SimpleStep Ignite™ ELISA kits and pay for only 3.
Offer valid until 12th August 2026. Not summable with other discounts.
Note: Inquire your discount on the product detail page.
New and unique!
abcam SimpleStep Ignite™ ELISA
50x Greater Sensitivity, Up to 35x Broader Measuring Range and Results in 55 Minutes
Abcam SimpleStep Ignite™ ELISA kits are next-generation chemiluminescent ELISA assays designed to deliver deeper insights from precious biological samples. With 50x greater sensitivity, up to 35x broader measuring range and a streamlined 55-minute workflow, SimpleStep Ignite™ helps quantify low- and high-abundance targets faster, with fewer dilutions and reduced hands-on complexity.
SimpleStep Ignite™ ELISA is especially valuable when sample volume is limited, target concentrations vary widely or protein levels are unknown. By increasing the probability that your target falls within the quantifiable range in the first assay, the broader measuring range and enhanced sensitivity help minimize repeated ELISA runs, reduce additional kit usage and support faster, more confident biomarker quantification.
Get to the answer faster. We’re redefining ELISAs to deliver accurate results in 55 minutes. That’s 6x faster than the industry standard making SimpleStep Ignite™ the fastest chemiluminescent ELISA on the market.
Minimize repeat ELISA runs when sample concentrations are unknown. 50x greater sensitivity and up to 35x broader measuring range increase the probability that your target protein falls within the quantifiable range in the first assay.
Consistency on repeat. Made with recombinant antibody pairs that deliver consistent performance batch to batch.
Less steps, more insight. With only one incubation and one wash step to streamline your workflow.
Dynamic range and Sensitivity
Example of increased sensitivity and dynamic range for Human Pro-Collagen I alpha 1 SimpleStep Ignite® ELISA kit (orange bar) (ab325360) compared to the corresponding colorimetric SimpleStep® ELISA kit and the leading colorimetric competitor kit. (SSE: SimpleStep® ELISA)
How does SimpleStep Ignite work?
SimpleStep Ignite™ ELISA relies on the same streamlined principle as our sandwich colorimetric SimpleStep® ELISA kits, with the same single-wash standardized workflow.
Chemiluminescent ELISA Technology for Deeper Insights
SimpleStep Ignite™ ELISA builds on the proven SimpleStep® sandwich ELISA principle while introducing chemiluminescent detection for enhanced performance. Chemiluminescent ELISA kits use light-emitting reactions for highly sensitive biomarker detection, providing stronger sensitivity and a broader dynamic range.
Designed for Precious Biological Samples
SimpleStep Ignite™ ELISA kits are suitable for a wide range of biological research samples, including serum, plasma, cell culture supernatants and CSF, depending on the individual kit validation. This makes them highly relevant for immunology, oncology, neuroscience, inflammation, metabolic research and translational biomarker studies.
Faster Workflow, Fewer Dilutions, More Reliable Results
- Quantify low-abundance biomarkers with greater confidence
- Reduce repeated dilutions and reruns
- Save valuable sample material
- Accelerate data generation
- Improve consistency across experiments
- Streamline ELISA workflows in busy laboratories