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Abnova - FISH probes and mutaFISH™ probes & accessories

Abnova has developed a range of FISH probes for the detection of gene amplification, loss and translocation, gene fusion and chromosomal aneuploidy. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library.

Abnova also provides a growing portfolio of validated mutaFISH™ probes (mutation-specific Fluorescence In Situ Hybridization) and accessory reagents for in situ, single cell, single molecule, DNA and RNA mutation detection at single nucleotide resolution.

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Fluorescence In Situ Hybridization (FISH) Probes

FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. Abnova provides a wide portfolio of FISH probes for identification of gene amplification, split, translocation, subtelomere aberration, prenatal chromosomal aberration and chromosomal markers. It is fast, sensitive, and specific.

Single, dual, and multiple colored probes for fast, sensitive, and specific detection
Work on metaphase spread, paraffin embedded & frozen tissue
High signal-to-noise ratio
Low cross-reactivity

FISH Product Lines

Single Color FISH Probes

Single color FISH probes are provided in 5X concentrated format to allow mixing of up to 5 single color FISH probes in a single hybridization assay.

Chromosome FISH Probes:

Identify specific chromosomes of interest. Each chromosome Available for FITC, Texas Red, Cy5, DEAC and R6G.

Subtelomere FISH Probes:

Detect subtle rearrangements. Available for FITC, Texas Red, Cy5, DEAC and R6G.

Dual Color FISH Probes

Dual color FISH probes are ready to use, each has a pair of locus-specific, fluorophore-labeled probes with different colors.

Gene FISH Probes:

For identification of specific gene amplification / gene loss.

Split FISH Probes:

For identification of gene split (break-apart of a gene).

Translocation FISH Probes:

For identification of gene translocation (gene fusion).

Multiple Color FISH Probes

Prenatal FISH Probes:

Aneuploidies of 5 chromosomes (13, 18, 21, X, Y) account for 95% of the chromosomal aberrations that cause infants born with defects. The products in this category each comprise of different combinations of fluorophore-labeled probes specific for chromosomes 13, 18, 21, X, and/or Y.

Procedure

mutaFISH™ probes (mutation-specific Fluorescence In Situ Hybridization)

Single Cell, Single Molecule, DNA and RNA Mutation Detection at Single Nucleotide Resolution

The ability to interrogate cellular heterogeneity at the single cell and single molecule levels has gained increased importance. Many bioreagents and techniques exist to address this issue separately. None has the unified approach to detect and quantify DNA and RNA changes across diverse spectrum – particularly point mutations and single-nucleotide polymorphisms (SNP) – while maintaining their spatial context. This challenge is even greater as different analytical platforms such fluorescence, cyto/histochemistry, flow cytometry, and microfluidics are needed to study the complexity of biological states.

Abnova has integrated padlock probe and rolling circle amplification (RCA) for in situ, single cell, single molecule, DNA and RNA mutation detection at single nucleotide resolution, and obviated the technical challenges of PCR and hybridization optimization required for efficient and target-specific analysis. Abnova provides a growing portfolio of off-the-shelf, validated mutaFISH™ probes and accessory reagents to address the unmet needs in the research and clinical settings.

mutaFISH™ Workflow

Advantages

Single Cell, Single Molecule Detection
In Situ Analysis of DNA & RNA
Single Nucleotide Resolution
Higher Sensitivity than dPCR and NGS
Multiplexing Capability
Cross Analytical Platforms
No DNA or RNA Extraction

mutaFISH™ Probe vs. Conventional DNA and RNA FISH Probe

	mutaFISH™ Probe	Conventional DNA FISH Probe	Conventional RNA FISH Probe
Point Mutation	Yes	No	No
Gene Amplification	Yes	Yes	No
Gene Deletion	Yes	Yes	No
Gene Translocation	Yes	Yes	No
Gene Expression	Yes	No	Yes

mutaFISH™ Application Examples

Cell In Situ Hybridization

mutaFISH™ staining was performed in situ in human CWR22Rv1 cells captured by CytoQuest™ CR. AR-V7 was detected via orange signal (Cy3), and ARwt was detected via red signal (Cy5).

Circulating Tumor Cell In Situ Hybridization

mutaFISH™ staining was performed in situ in CTCs from NSCLC patient. CTCs were detected by using immunofluorescence staining for CSV (FITC, green). EGFR L858R point mutation was detected via orange signal (Cy3) and EGFR Ex19wt was detected purple signal (Texas Red).

Fresh Frozen Tissue In Situ Hybridization

mutaFISH™ staining was performed in situ in human frozen lung adenocarcinoma tissue. EGFR L858R point mutation was detected via orange signal (Cy3), EGFR L858wt was detected via red signal (Cy5), and EGFR Ex19wt was detected via green signal (Texas Red).

Formalin-Fixed Paraffin-Embedded Tissue In Situ Hybridization

mutaFISH™ staining was performed in situ in human breast cancer FFPE tissue. HER2 was detected via red signal (Cy5).

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