Abnova NanoAb™ Service
VHH antibody, also known as Camelid single domain antibody (sdAb), is the recombinant variable domain derived from camelid heavy-chain-only antibody. This configuration confers some unique properties. The small size and high stability of VHH antibodies make it a promising tool for disease diagnosis and treatment. VHH antibodies can penetrate tissue more easily and target difficult epitopes hidden in the protein structure. Additionally, VHH antibodies express well in many host systems, making it cost-effective for large-scale manufacturing.
Abnova offers the NanoAb™ service for the production of target-specific VHH antibodies from the camelid VHH library. Using advanced camelid VHH library screening technology, we screen the library and isolate the VHH clones for your required antigens. Positive clones are sequenced and validated by ELISA. The selected clones will then be introduced into the mammalian expression vector for scalable VHH antibody production, suitable for many downstream applications. The bioactivity of NanoAb™ VHH antibodies expressed in mammalian cells is guaranteed.
Specification
Naïve Camelid VHH Library | Immune Library | |
|---|---|---|
Library Size | 1.2 x 1012 | 1.2 x 1013 |
Required Screening Antigen Amount | 0.6 mg | 3.5 mg |
Lead Time | 8-10 weeks | 22-24 weeks |
Deliverables
|
1. Phage validation through phage ELISA data 2. Up to 5 different VHH sequences 3. Up to 1 purified VHH, approximately 0.2 mg per VHH | 1. cDNA 150ug 2. VHH library 3-5ml 3. Phage validation through phage ELISA data 4. Up to 8 different VHH sequences 5. Up to 1 purified VHH, approximately 0.2 mg per VHH |
Advantages
- Small Size
- Superior Stability
- Low Immunogenicity
- High Affinity and Specificity
- Fast Blood Clearance
- Versatile Production
- Easy Multimerization (Multivalency / Multiparatopicity / Multispecificity)
- Easy Conjugation
- Robustness in Bioengineering
- Applications Across Fields
VHH Antibody vs. Conventional Mab and Pab
VHH Antibody | Mab | Pab | |
|---|---|---|---|
Size | 12 – 15 kD | Approximately 150 kD | Approximately 150 kD |
Required Antigen Amount | ≦ 1 mg | ≦ 4 mg | ≦ 4 mg |
Method | Camelid VHH library screening | Hybridoma technology | Harvested in antiserum |
Stability | High stability to heat, pH, and denaturing agents | Thermal instability | Biological instability |
Lot-to-Lot Consistency | Very high | High | Low |
Lead Time | 8-24 weeks | 16-24 weeks | 4-12 weeks |
Examples
CD8A VHH Humanized Monoclonal Antibodies
Western Blot
Flow Cytometry
Human EpCAM VHH Phage Supernatant
Flow Cytometry
Mouse CAIX VHH Phage Supernatant
Flow Cytometry
Mouse CD276 VHH Phage Supernatant
Flow Cytometry
Mouse PD-L1 VHH Phage Supernatant
Flow Cytometry
