Absolute Antibody Custom Services -
Sequencing, Engineering & Recombinant Expression
Absolute Antibody, now part of Vector Laboratories, is an ISO 9001:2015–certified manufacturer that has the vision to make recombinant antibody technology accessible to all.
Absolute Antibody provides custom services and thereby specializes in the sequencing, engineering, and recombinant manufacture of antibodies. Secure, adjust or improve your own antibodies! Engineer or have produced your specific antibody!
Antibody Sequencing
Sequence from hybridoma cells or from purified antibody protein. Sequence your antibody to enable recombinant production or to obtain intellectual property rights for your sequence!
Hybridoma Sequencing
Starting point for protecting your antibody and embarking on antibody engineering projects such as species, isotype and subtype switching or even humanization.
Antibody Protein Sequencing
If the cell line has been lost or is otherwise unavailable, but sufficient protein is still available, advances in mass spectrometry technology have made it possible to sequence the protein.
Polyclonal Antibody Sequencing
Immortalization service to secure a precisely defined and reproducible source of your antibodies.
Antibody Engineering
Rapidly reformat your antibody into any species or isotype. Services include humanization and custom bispecifics.
Absolute Antibody has developed a proprietary cloning system to enable rapid reformatting of antibodies into almost any format. Their antibody engineering services include chimerization, humanization, isotype switching and the manufacturing of recombinant antibody fragments and bispecific antibodies.
Other Species
Currently Absolute Antibody has antibody sequences available for a large number of animals ranging from pig, sheep, horse, dog, chicken, fish, platypus and a whole range of non-human primates. Moreover, their experts are able to determine antibody sequences from most genomes (even if only partial assemblies are available).
Chimerization
Absolute Antibody offers an antibody chimerization service which enables you to create a chimeric antibody, consisting of the original variable domains from your antibody with the constant domains from a different species, for almost any mammalian species.
See also chimeric antibodies
Humanization
Absolute Antibody offers a royalty-free antibody humanization service. Absolute Antibody’s Prometheus™ antibody humanization service delivers a panel of humanized variants from your clone, guaranteeing to produce a variant with comparable activity to the parent antibody.
See also antibody humanization
Antibody fragments
In certain situations antibody fragments offer an ideal alternative to full-length antibodies. Absolute Antibody’s proprietary recombinant expression and antibody engineering platform enables to convert any antibody into a fragment, such as a single chain Fv (scFv), Fab or Fab2, followed by expression and purification at any scale.
See also antibody fragments page
Isotype switching
Absolute Antibody’s isotype switching service (also known as class switching) allows you to alter the isotype or subtype of any antibody. This can be used to alter the in vivo effector function and stability of an antibody, to overcome e.g. aggregation properties or to convert your IgG to an IgM for increased avidity.
Bispecific antibodies
Absolute Antibody offers a bispecific antibody service. They can reformat any pair of antibodies into an IgG-scFv format (i.e. an IgG with a scFv fused to the C-terminus of the IgG). This results in a bispecific bivalent antibody format capable of binding two antigens simultaneously.
Engineered Fc domains
Absolute Antibody can engineer the Fc domain of your antibody to mediate effector function or extend the plasma half-life. For example, they can abolish Fc domain binding to FcγR using our Fc Silent™ mutation or mAbsolve’s STR mutations, as well as extend or shorten the plasma half-life of your IgG antibody by introducing mutations in the Fc domain.
Codon Optimization
Codon optimization can enhance the expression of recombinant antibodies. By incorporating this service into antibody development projects, our clients can significantly enhance their antibody expression yield during the antibody engineering and recombinant expression processes.
...and more
The Periodic Table of Antibodies shown below gives you an impression possible antibody formats .
Antibody Expression
Produce your antibody in Absolute Antibody’s recombinant expression system at milligram-to-gram scale.
Absolute Antibody’s antibody expression platforms rapidly produces high-quality, animal-free recombinant antibodies at milligram-to-gram scale in HEK or CHO cells. Their serum-free mammalian transient expression system allows to manufacture recombinant antibodies in any species, isotype or format with high purity and low endotoxin levels. You can start from an electronic antibody sequence, hybridoma cell line or purified antibody protein.
All production occurs at ISO 9001:2015-certified manufacturing facility.
All antibody expression services are royalty-free and customizable to your needs.
HEXpress™ HEK Antibody Production
HEXpress™ service produces milligram-to-gram quantities of recombinant antibodies in HEK cells with high purity and low endotoxin levels. This service provides a faster, more affordable alternative to stable cell line generation, with proven expression of antibodies in any species, isotype or format. Read more
CHXpress™ CHO Antibody Production
CHXpress™ service enables serum-free mammalian transient expression in CHO cells to better serve clients through their full antibody development. This service is particularly useful for bridging the gap to stable CHO production, or for tackling challenging constructs. Read more
FleXpress™ High-Throughput Antibody Production
FleXpress™ service allows to rapidly express large numbers of IgG antibodies at 80 ml scale using the proven recombinant antibody technology. Particularly useful for early-stage pilot studies on a group of therapeutic antibody candidates, as well as converting a monoclonal antibody portfolio from hybridomas to recombinant production. Read more