Amiprox –
Advanced Plasma Sample Preparation for Proteomics
Deplete abundant proteins. Discover more of the proteome.
Plasma proteomics holds valuable insights, but highly abundant proteins can mask low-abundance biomarkers. Amiprox simplifies this challenge with ready-to-use ProtiPrep™ spin columns and ProtiPlate™ 96-well plates for efficient human serum albumin (HSA) and immunoglobulin G (IgG) depletion. With streamlined 10-minute workflows, low sample input and reproducible performance, these solutions support sensitive mass spectrometry analysis, biomarker discovery and scalable plasma research.
Overcome the Challenges of Plasma Proteomics
Human serum albumin (HSA) and immunoglobulin G (IgG) together account for nearly 70% of total plasma protein content. Their high abundance can mask lower-abundance proteins, limiting the depth of proteomic analysis and making biomarker identification more challenging. Traditional depletion methods often require multiple preparation, equilibration and washing steps, increasing hands-on time and introducing variability.
Amiprox provides an efficient alternative, combining preloaded affinity resins, integrated buffers and streamlined workflows to facilitate reproducible plasma protein depletion without extensive sample preparation.
Key Advantages of Amiprox Proteomics Solutions
- Rapid Sample Preparation: Efficient depletion of highly abundant plasma proteins in just 10 minutes.
- High Depletion Efficiency: Over 97% removal of HSA and IgG with ProtiPrep™ Pop2.
- Minimal Sample Input: Only 10 µL of plasma required per sample.
- Simplified Workflow: Ready-to-use systems with integrated buffers eliminate time-consuming preparation and washing steps.
- Reliable & Reproducible Results: Standardized workflows reduce handling variability and support consistent sample preparation.
- Flexible Throughput: From individual spin columns to automation-compatible 96-well plates.
ProtiPrep™ – Rapid Protein Depletion in Spin Columns
Simplify plasma preparation. Maximize discovery.
ProtiPrep™ is an innovative spin-column system designed for fast, reproducible depletion of abundant plasma proteins. Its patented design integrates affinity resins and prefilled buffer compartments into a ready-to-use device.
With minimal pipetting and no separate buffer preparation, ProtiPrep™ simplifies sample processing while improving accessibility to low-abundance proteins for downstream proteomic analysis.
Available ProtiPrep™ Solutions
- ProtiPrep™ Pop1: Selective removal of human serum albumin (HSA).
- ProtiPrep™ Pop2: Simultaneous depletion of HSA and IgG using Protein A-based capture.
- ProtiPrep™ Pop2+: HSA and IgG depletion using Protein G for broader IgG-binding compatibility across species.
ProtiPrep™ Pop2 – Performance Highlights
- 97.27% HSA depletion and 98.62% IgG depletion in the reported ELISA evaluation.
- 10-minute workflow using just 10 µL of human plasma.
- Approximately 100 additional protein groups identified after depletion in a comparative mass spectrometry experiment.
- Convenient spin-column design requiring only standard laboratory equipment.
ProtiPlate™ – High-Throughput Plasma Sample Preparation
One plate. Multiple samples. Reproducible results.
For laboratories processing larger sample cohorts, ProtiPlate™ transfers the proven Amiprox depletion technology into prefilled 96-well deep-well plates.
Designed for both manual processing and integration with automated liquid handling systems, ProtiPlate™ enables efficient HSA and IgG depletion across multiple plasma samples while minimizing hands-on preparation and well-to-well variability.
Available ProtiPlate™ Solutions
- ProtiPlate Pop1: High-throughput removal of HSA.
- ProtiPlate Pop2: Simultaneous depletion of HSA and IgG in a 96-well format.
ProtiPrep™ Pop2 – Performance Highlights
- 98.6% mean HSA depletion and 97.6% mean IgG depletion in the reported ELISA evaluation.
- 96-well deep-well format for scalable sample processing.
- Only 10 µL of undiluted plasma per well.
- Approximately 10-minute preparation workflow.
- Compatible with automated liquid handling systems.
- Consistent depletion across sample batches.