baseclick - Click Chemistry for Bioconjugation
Superior Cell Proliferation Assays
Simple labelling method | ClickTech EdU Cell Proliferation and T Cell Proliferation Kits
baseclick is the inventor of click chemistry, the most streamlined and simplified bioconjugation method in life science. The breakthrough in CuAAC or SPAAC, the so-called click reaction, results in a convenient, versatile and reliable single step covalent coupling. Simple or no work-up and no purification are required!
ClickTech EdU cell proliferation and T cell proliferation assays provide a superior alternative to BrdU assays. For monitoring of cell vitality, determining genotoxicity or evaluating anticancer drugs.
Click Chemistry Technology Highlights
- Improve your cell proliferation assays with EdU Cell Proliferation Kits, a superior alternative to BrdU assays
- Modify your oligos, DNA and RNA – Enables labelling with up to 99.99% yield!
- Discover clickable dyes, tags, sugars, fatty acids, amino acids, biotin etc.
- Profit from 5’, 3’, dual, multi or internally labeled DNA or RNA sequences used in FISH & PCR Kits
- Profit from click chemistry in NGS – Enables – for the first time – complete full-length information from 5’ to 3’ in transcriptomics and is compatible with all available sequencing devices!
baseclick Promises
- High Purity: Provides products with high purity
- High Quality: Analyzed by HPLC, LCMS and NMR measurement
- High Activity: The high purity is a guarantee for the biological activity
ClickTech EdU Cell Proliferation Kits
A superior alternative to BrdU assays
Cell proliferation is characterized by de novo DNA synthesis during the S-Phase of the cell cycle. Measurement of DNA synthesis is therefore essential for assessing cell health, monitoring cell vitality, determining genotoxicity and evaluating the impact of drugs on cell cycle or cancer.
The traditional method uses the nucleoside analoge BrdU to be incorporated into newly transcribed DNA. Instead, baseclick’s ClickTech EdU cell proliferation kits and ClickTech EdU T cell proliferation kits are provided with the nucleoside analoge EdU (5-ethynyl-2-deoxyuridine). EdU is added to the cells and incorporated into newly synthesized DNA during active cell division. This nucleoside contains a reactive alkyne group (instead of the bromine group in BrdU) and is thus capable of undergoing a so-called click reaction.
Using the nucleoside analoge EdU to be incorporated into DNA during active DNA synthesis, has several advantages over the BrdU method:
NOTE: All EdU based cell proliferation kits on the market are patented by baseclick!
Advantages of ClickTech EdU over the BrdU method
ClickTech EdU method - Quick and gentle
ClickTech EdU assays measure the rate of new DNA synthesis based on the integration of nucleoside analog EdU (5-ethynyl-2’-deoxyuridine) into DNA.
Detection is via a catalyzed „click“ reaction that is typically completed within 30 minutes. The click reaction fluorescently labels proliferating cells. Only actively dividing cells are measured.
- Simpler procedure with less working steps
- Time to detection = 1 hour
- Less cytotoxic, gentle reaction conditions
- No toxic effects in animals
Result:
ClickTech EdU method accurately determines cell proliferation while preserving cell morphology, DNA integrity and antigen binding sites. ClickTech EdU method produces low background and high detection sensitivity.
BrdU method - Lengthy and harsh
The traditional method uses the nucleoside analog BrdU (5-bromo-2′-deoxyuridine, a nucleoside analog of thymidine), which is incorporated into newly transcribed DNA.
After incorporation, samples are treated with aggressive methods (HCl, heat, or enzymes) to denature the DNA and expose the BrdU molecules for detection with anti-BrdU antibodies.
- Time consuming, includes more procedure steps
- Time to detection = 4 hours
- Performance may be inconsistent
- Harsh treatment necessary to give anti-BrdU antibody access to the incorporated BrdU nucleosides
Result:
Harsh treatments required for BrdU methods may affect sample integrity, cell morphology, image quality, and multiplexing capability.
baseclick ClickTech EdU Product Portfolio
EdU Nucleosides
Clickable EdU allows detection of de novo DNA. EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and can be incorporated into DNA during active DNA synthesis. For convenient fluorescent read-outs to assess cell proliferation, cell cycles, viability, etc.
EdU Nucleosides - Price Comparison
ClickTech EdU Cell Proliferation Kits
Direct measurement of cell proliferation and differentiation between actively dividing cells and quiescent cells. Allows differentiation of the various stages of S-phase by the different replication patterns.
baseclick offers its superior ClickTech EdU cell proliferation assays for different readout systems:
*Kits available in a version for in vivo application
All kits are supplied with either 6-FAM-Azide (488, FITC, GFP channel), 5-TAMRA-PEG-3-Azide (555, Cy3 analoge), 5/6 Sulforhodamine 101-PEG-3-Azide (594, Texas Red® alternative) or Eterneon Red 645 Azide (647, Cy5 filter setup).
Benefits of ClickTech Edu Cell Proliferation Kits
- Direct viability of cell cycle
- Fast detection procedure (30 min)
- Mild conditions, no DNA denaturation required
- Superior alternative to BrdU assays
- Monitoring genotoxicity
- Evaluation of anticancer drugs
- High quantitative yields
- No false positives
- Easy to perform
- Highly reliable and efficient method
ClickTech Edu Cell Proliferation Assay Procedure
EdU (5-ethynyl-2-deoxyuridine), is added to the cells and incorporated into newly synthesized DNA during active cell division via its reactive alkyne group (= click reaction). After an incubation period, the cells are washed, fixed and permeabilized in order to subsequently be able to take up the detection reagents. The detection is then carried out via the so-called copper catalyzed click reaction (CuAAC)-
EdU Cell Proliferation Kits - Price Comparison
| Baseclick (Lucerna-Chem AG) | ThermoFisher (Technology licensed from Baseclick) | |
| ClickTech EdU Cell Proliferation Kit 488 for Imaging 5mg EdU, 100 Assays (0.5ml reaction volume) |
594.- (BCK-EdU488IM100) also available for 555, 594 and 647 |
> 1100.- (C10337) |
| ClickTech EdU Cell Proliferation Kit 488 for HTS 2x 2ml of 20x conc. EdU, 200 Assays |
436.- (BCK-EdU488HTS2) also available for 555 |
> 1200.- (C10350) |
| ClickTech EdU Cell Proliferation Kit 488 for FC 4x 5mg EdU, 100 Assays |
961.- (BCK-EdU488FC100) also available for 555, 594 and 647 |
> 1700.- (C10419) |
ClickTech EdU T Cell Proliferation Kits
Sensitive and less cytotoxic assay (compared to BrdU) for identification of actively dividing T cells. Allows quantitative measurement of cell division of different T lymphocyte subsets by multicolor labelling.
Benefits of ClickTech Edu T Cell Proliferation Kits
- EdU outperforms the standard BrdU T cell proliferation detection
- Less cytotoxic for human T cells
- Offers superior signal-to-background ratio
- Less time consuming than dye dilution methods
- Allows for better discernable interferon gamma responses
- Can be applied at any time point of your experiment
- Offers the possibility of multiplexing
ClickTech Edu T Cell Proliferation Kit Procedure
EdU (5-ethynyl-2-deoxyuridine) is incorporated into DNA during active DNA synthesis over the course of 2 hours before detection The click bioconjugation reaction is complete within 30 minutes and is compatible with multiplexing for content and context rich results.
EdU vs. Dilution-based T Cell Proliferation assay
Ordinarily, detection of T cell proliferation is performed using dilution-based methods, such as the use of fluorescent cell tracking reagents. This approach can reduce T cell proliferation, viability, and responsiveness, and cell proliferation can only be determined after a completed round of cell division. ClickTech EdU T cell proliferation Kit overcomes the limitations of dilution-based T cell proliferation probes (CellTrace Far Red), and thus provides a superior alternative for measuring T cell proliferation.
Stimulation of human T cell activation.
