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30% off Protein Modification Products
Click Chemistry to modify your proteins and peptides
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baseclick - Click Chemistry for Bioconjugation
Simple labelling method | ClickTech EdU Cell Proliferation and T Cell Proliferation Kits
baseclick is the inventor of click chemistry, the most streamlined and simplified bioconjugation method in life science. The breakthrough in CuAAC or SPAAC, the so-called click reaction, results in a convenient, versatile and reliable single step covalent coupling. Simple or no work-up and no purification are required!
ClickTech EdU cell proliferation and T cell proliferation assays provide a superior alternative to BrdU assays. For monitoring of cell vitality, determining genotoxicity or evaluating anticancer drugs.
Technology Highlights of Click Chemistry
Modify your oligos, DNA & RNA, protein & peptides | Enables labelling up to 99.99% yield!
- The most versatile click chemistry portfolio of chemical tools
- Clickable dyes, tags, sugars, fatty acids, amino acids, biotin etc.
- Improved cell proliferation assays (EdU Cell Proliferation Kits) providing a superior alternative to BrdU method
- Click chemistry in Next Generation Sequencing enables – for the first time – complete full-length information from 5′ to 3′ in transcriptomics and is compatible with all available sequencing devices.
- 5’, 3’, dual, multi or internally labeled DNA or RNA sequences used in FISH & PCR
baseclick Promises
- High Purity: Provides products with high purity
- High Quality: Analyzed by HPLC, LCMS and NMR measurement
- High Activity: The high purity is a guarantee for the biological activity
30% off selected products
Protein Modification Products
ClickTech EdU Cell Proliferation Kits
An equivalent alternative to BrdU assays
NOTE: All EdU based cell proliferation kits on the market are patented by baseclick!
Cell proliferation is characterized by de novo DNA synthesis during the S-Phase of the cell cycle. Measurement of DNA synthesis is therefore essential for assessing cell health, monitoring cell vitality, determining genotoxicity and evaluating the impact of drugs on cell cycle or cancer.
The traditional method uses the nucleoside analoge BrdU to be incorporated into newly transcribed DNA. Instead, baseclick’s ClickTech EdU cell proliferation kits and ClickTech EdU T cell proliferation kits are provided with the nucleoside analoge EdU (5-ethynyl-2-deoxyuridine). EdU is added to the cells and incorporated into newly synthesized DNA during active cell division. This nucleoside contains a reactive alkyne group (instead of the bromine group in BrdU) and is thus capable of undergoing a so-called click reaction.
Using the nucleoside analoge EdU to be incorporated into DNA during active DNA synthesis, has several advantages over the BrdU method:
ClickTech EdU method vs. BrdU method
ClickTech EdU method - Quick and gentle
ClickTech EdU assays measure the rate of new DNA synthesis based on the integration of nucleoside analog EdU (5-ethynyl-2’-deoxyuridine) into DNA.
Detection is via a catalyzed „click“ reaction that is typically completed within 30 minutes. The click reaction fluorescently labels proliferating cells. Only actively dividing cells are measured.
- Simpler procedure with less working steps
- Time to detection = 1 hour
- Less cytotoxic, gentle reaction conditions
- No toxic effects in animals
Result:
ClickTech EdU method accurately determines cell proliferation while preserving cell morphology, DNA integrity and antigen binding sites. ClickTech EdU method produces low background and high detection sensitivity.
BrdU method - Lengthy and harsh
The traditional method uses the nucleoside analog BrdU (5-bromo-2′-deoxyuridine, a nucleoside analog of thymidine), which is incorporated into newly transcribed DNA.
After incorporation, samples are treated with aggressive methods (HCl, heat, or enzymes) to denature the DNA and expose the BrdU molecules for detection with anti-BrdU antibodies.
- Time consuming, includes more procedure steps
- Time to detection = 4 hours
- Performance may be inconsistent
- Harsh treatment necessary to give anti-BrdU antibody access to the incorporated BrdU nucleosides
Result:
Harsh treatments required for BrdU methods may affect sample integrity, cell morphology, image quality, and multiplexing capability.
baseclick ClickTech EdU Product Portfolio
EdU Nucleosides
Clickable EdU allows detection of de novo DNA. EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and can be incorporated into DNA during active DNA synthesis. For convenient fluorescent read-outs to assess cell proliferation, cell cycles, viability, etc.
EdU Nucleosides - Price Comparison
Pay 6x less for exactly the same product!
ClickTech EdU Cell Proliferation Kits
Direct measurement of cell proliferation and differentiation between actively dividing cells and quiescent cells.
Allows differentiation of the various stages of S-phase by the different replication patterns.
Benefits of ClickTech Edu Cell Proliferation Kits
- Direct viability of cell cycle
- Fast detection procedure – only 30 min
- Mild conditions, no DNA denaturation required
- Superior alternative to BrdU assays
- Monitoring genotoxicity
- Evaluation of anticancer drugs
- High quantitative yields
- No false positives
- Easy to perform
- Highly reliable and efficient method
ClickTech Edu Cell Proliferation Assay Procedure
EdU (5-ethynyl-2-deoxyuridine), is added to the cells and incorporated into newly synthesized DNA during active cell division via its reactive alkyne group (= click reaction). After an incubation period, the cells are washed, fixed and permeabilized in order to subsequently be able to take up the detection reagents. The detection is then carried out via the so-called copper catalyzed click reaction (CuAAC)-
Kit Formats - available for different Read-Outs
baseclick superior ClickTech EdU cell proliferation assays are available for different readout systems and with different labels:
- Cell Proliferation Kits for Imaging and for in vivo Imaging
- 488, 555, 594 or 647 available
- Cell Proliferation Kits for Flow Cytometry (FC) and for in vivo FC
- 488, 555, 594 or 647 available
For increased sensitivity:
brand new fluorescence enhancer system providing outstanding efficiency and greatly enhanced signal and sensitivity.
Different Read-Outs
In vivo
application
Imaging / Microscopy
Flow
Cytometry
High-throughput-Screening (HTS)
Different Fluorophores
488: 6-FAM-Azide (FITC, GFP channel)
555: 5-TAMRA-PEG-3-Azide (Cy3 analoge)
594: 5/6 Sulforhodamine 101-PEG-3-Azide (Texas Red® alternative)
647: Eterneon Red 645 Azide (Cy5 filter)
EdU Cell Proliferation Kits - Price Comparison
Save more than 50% for exactly the same Kit!
Baseclick |
ThermoFisher Click-iT™ EdU Cell Proliferation Kit Technology licensed from Baseclick |
|
| EdU Cell Proliferation Kit 488 for Imaging 5mg EdU, 100 Assays (0.5ml reaction volume) |
624.- (BCK-EdU488IM100) also available for 555, 594 and 647 |
> 1190.- (C10337) |
| EdU Cell Proliferation Kit 488 for FC 4x 5mg EdU, 100 Assays |
997.- (BCK-EdU488FC100) also available for 555, 594 and 647 |
> 1770.- (C10419) |
| EdU Cell Proliferation Kit 488 for HTS 2ml of 20x conc. EdU, 200 Assays |
457.- (BCK-EdU488HTS2) also available for 555 |
> 1240.- (C10350) |
| Save 50% even for the format with enhanced sensitivity! | ||
| Sensitive EdU Cell Proliferation Kit 488 for Imaging 5mg EdU, 100 Assays |
663.- (BCK-EdUPro488IM100) also available for 647 |
- |
| Sensitive EdU Cell Proliferation Kit 647 for FC 5mg EdU, 50 Assays |
688.- (BCK-EdUPro647FC50) also available for 488 |
- |
| Sensitive EdU Cell Proliferation Kit 488 for HTS 2ml of 20x conc. EdU, 200 Assays |
495.- (BCK-EdUPro488HTS2) also available for 555 |
- |
ClickTech EdU T Cell Proliferation Kits
Sensitive and less cytotoxic assay (compared to BrdU) for identification of actively dividing T cells. Allows quantitative measurement of cell division of different T lymphocyte subsets by multicolor labelling.
Benefits of ClickTech Edu T Cell Proliferation Kits
- EdU outperforms the standard BrdU T cell proliferation detection
- Less cytotoxic for human T cells
- Offers superior signal-to-background ratio
- Less time consuming than dye dilution methods
- Allows for better discernable interferon gamma responses
- Can be applied at any time point of your experiment
- Offers the possibility of multiplexing
ClickTech Edu T Cell Proliferation Kit Procedure
EdU (5-ethynyl-2-deoxyuridine) is incorporated into DNA during active DNA synthesis over the course of 2 hours before detection The click bioconjugation reaction is complete within 30 minutes and is compatible with multiplexing for content and context rich results.
EdU vs. Dilution-based T Cell Proliferation assay
Ordinarily, detection of T cell proliferation is performed using dilution-based methods, such as the use of fluorescent cell tracking reagents. This approach can reduce T cell proliferation, viability, and responsiveness, and cell proliferation can only be determined after a completed round of cell division. ClickTech EdU T cell proliferation Kit overcomes the limitations of dilution-based T cell proliferation probes (CellTrace Far Red), and thus provides a superior alternative for measuring T cell proliferation.
Stimulation of human T cell activation.
Discover further baseclick Click Chemistry Products
From classic Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) to strain-promoted azide-alkyne cycloaddition (SPAAC) and beyond, click chemistry reagents inculde a versatile array of compounds designed for efficient, selective, and high-yielding reactions in chemical synthesis. These reagents enable the rapid and robust construction of complex molecular architectures used in drug discovery, materials science and bioconjugation.
EdU cell proliferation assays provide a reliable method for quantifying cellular proliferation and assessing the effects of various compounds, treatments, or conditions on cell growth. Based on the incorporation of 5-ethynyl-2′-deoxyuridine (EdU) into newly synthesized DNA, these assays offer high sensitivity, minimal background, and compatibility with diverse cell types and experimental setups.
Modify DNA and RNA molecules to gain new insights into gene function, regulation, and disease mechanisms. From labelling techniques for advanced imaging to site-specific modifications for targeted therapies, baseclick allows you to tailor nucleic acids with superior precision and efficiency.
Discover this product catalog and improve your protein research with azide-modified amino acids that enable protein labelling and bioconjugation.
Enable conjugation of nucleic acids with other biomolecules using our specialized bioconjugation reagents, allowing for the creation of complex molecular structures and targeted delivery systems.
baseclick offers mRNA service and a customised oligonucleotide service for cutting-edge molecular biology research tailored to your specifications.
