Bio-Helix: Protein Detection Products
Bio-Helix offers a range of high quality protein characterisation tools including Protein Ladders for SDS-PAGE, electrophoresis stains such as Coomassie blue or Ponceau S as well as ECL Luminescence Substrates for protein detection in Western Blots.
Featured Products
COOMASSIE Nano Protein Staining Solution
Protein Ladders / Markers
OneStep Blocker - Western Blocking & Enhancer
UltraScence Western Substrates, ECL
COOMASSIE Nano Protein Staining Solution
- Ready-to-use protein staining solution for SDS-PAGE gels
- Ultra-Fast protein detection (5 min.)
- No risk of overstaining (e.g. overnight incubation)
- High sensitivity down to 10ng
- No need for destaining
- No washing steps
- Safe and environmentally friendly (no methanol, no acetic acid)
- Compatible with mass spectrophotometry
Formats:
1x conc. 500ml
5x conc. 1L (gives 5L of 1x conc.)
– no loss of performance upon dilution
– more convenient, cheaper and environmental friendlier shipping
Coomassie Staining - Price Comparison
| Supplier | Product | Cat# | Conc. | Qty | List Price CHF | Listprice per 1 L (1x conc.) |
| Bio-Helix | Coomassie Nano | PS002-B500ML | 1x | 0.5 L | 78 CHF | 156 CHF |
| 5x Coomassie Nano | PS002-L05X | 5x | 1 L (5x conc.) | 442 CHF | 88.40 CHF | |
| LubioScience | QuickBlue Protein Stain | LU001000 | 1x | 1 L | 160 CHF | 160 CHF |
| Bio-Rad | Bio-Safe™ | 1610786 | 1x | 1 L | 192 CHF | 192 CHF |
| Abcam | InstantBlue® | ab119211 | 1x | 1 L | 203 Euro | 203 Euro |
| ThermoFisher | PageBlue™ | 24620 | 1x | 1 L | 193 CHF | 193 CHF |
| SimplyBlue™ | LC6060 | 1x | 1 L | 346 CHF | 346 CHF | |
| LC6065 | 1x | 3.5 L | 828 CHF | 276 CHF |
COOMASSIE Nano Protocol Tips
It is important NOT to wash the gel!
Protocol Tips for Ultra Fast Staining
Protocol Tips for Superior Visibility Staining
COOMASSIE Nano - Fast Staining and High Sensitivity
Fast staining within 5 Min.
COOMASSIE Nano Protein Staining Solution visualizes protein bands already within the first 5 minutes of staining. No overstaining occurs after overnight incubation.
High Sensitivity
COOMASSIE Nano Protein Staining Solution detects proteins with high sensitivity down to 10 ng (Lane H).
Benchmark: COOMASSIE Nano performs equivalent or better compared to other brands
Equivalent or even superior performance
COOMASSIE Nano Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands.
Lightly agitate the staining box for 5 to 30 minutes at room temperature.
Protein Ladders
Bio-Helix provides a comprehensive portfolio of high-quality protein ladders for SDS-PAGE and/or Western Blots.
Loading Quantity Suggestions
- 3 μl on 15-well mini-gels
- 5 μl on 10-well mini-gel
- 2.5 μl for general Western transferring
Stability / Storage
- Stable for up to 2 weeks at 25°C.
- Stable for up to 3 months at 4°C.
- Stable for up to 24 months at -20°C.
Blu10 Plus Prestained Protein Ladder (BLUltra)
6.5 to 270 kDa
IRIS11 Prestained Protein Ladder
3 to 260 kDa
11 pre-stained proteins from 3 to 260 kDa. The recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. Read more
UNveil Unstained Protein Ladder
10 to 200 kDa
12 unstained recombinant proteins from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. Read more
OneStep Blocker - Western Blocking Solution and Signal Enhancer
The OneStep Blocker serves as both blocker and enhancer in Western analysis. With the 3-in-1 step procedure, OneStep Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.
- Perform 3 steps in 1: Membrane blocking + 1o Ab incubation + hybridization with 2o Ab
- Enhance antibody signal 2- to 5-fold if developed with HRP or AP substrates (= increased sensitivity)
A novel way to perform Western Blot
Mix OneStep Blocker, 1°Ab and 2° Ab with membrane together, that's all.
- Block + incubate/hybridize antibodies in 1 step
- Save at least 2 hours
- Less hands-on steps
- Enhance antibody signal (2-5x increase)
- Reduce overall background
- Minimize non-specific signals
- Compatible with PVDF & NC membrane
- Effective with any ECL
UltraScence Western Substrates, ECL
Bio-Helix UltraScence Pico and Femto Western Substrates are luminol-based enhanced chemiluminescence (ECL) substrates with long signal duration and offer the perfect choice for performing immunoblotting with horseradish peroxidase (HRP)-conjugated secondary antibodies. Read more
- No protocol change when switching from other brands to UltraScence
- 2-year storage at room temperature without affecting performance
- Different sensitivities, optimized for different protein amounts available
- Enhanced chemiluminescence signal duration
- Optimized for use with PVDF and Nitrocellulose membranes.
- Compatible with Western Blotting Markers.
- Optimized for film and CCD-based imaging
UltraScence Pico Plus Western Substrate
low picogram or high femtogram detection
UltraScence Pico Ultra Western Substrate
low picogram or mid femtogram detection
UltraScence Femto Western Substrate
mid femtogram to low femtogram detection
UltraScence Femto Plus Western Substrate
low femtogram detection (mid-atto)
Benchmark:
Ultrascence Pico Plus compared to other brands
UltraScence Pico Plus Western Substrate enables an accurate low picogram to high femtogram detection.
Serial dilutions of EGFP were applied in electrophoresis. After protein transfer, membranes were probed with GFP tag Rabbit PolyAb followed by Goat Anti-rabbit IgG/HRP secondary antibody. Blots were incubated with UltraScence Pico Plus Western substrate. The blots were and were simultaneously exposed for 5, 10, 30, 60 and 180 seconds.
Benchmark:
Ultrascence Femto and Femto Plus compared to other brands
UltraScence Femto Plus Western Substrate enables an accurate low picogram to high femtogram detection.
Serial dilutions of EGFP were applied in electrophoresis. After protein transfer, membranes were probed with GFP tag Rabbit PolyAb followed by Goat Anti-rabbit IgG/HRP secondary antibody. Blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375) and were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds and 180 seconds.
