Cube Biotech - Accurate and targeted protein research

The INDIGO His-tag purification method by Cube Biotech offers distinct advantages over traditional resins like Ni-NTA. INDIGO resins are engineered for stability in challenging conditions. Ni-INDIGO features a high binding capacity (>100 mg/mL) and maintains stability across extreme conditions, including resistance to 20 mM EDTA and DTT. This increased resilience minimizes non-specific binding, enhancing the purity and yield of His-tagged proteins. Furthermore, INDIGO products reduce metal ion leaching, maintaining consistent binding capacity and improving experimental reproducibility. These properties make INDIGO ideal for applications where protein integrity and precise downstream processing are critical.

INDIGO Technology is available in both agarose and magnetic bead forms and is highly suited for challenging samples that require robustness and stability under harsh conditions. Pre-packed cartridges and bulk volumes allow seamless scale-up from lab research to industrial production. 

• High Stability: INDIGO withstands strong chelators and reducing agents (e.g., DTT, EDTA) as well as pH extremes (4-13), enabling flexibility in experimental setups.

• Improved Yield and Purity: Its robust nickel-binding coordination reduces metal leaching and yields consistently high purity proteins, outperforming many competitors.

• High-Throughput Ready: The resins are optimized for high-throughput applications, facilitating efficient workflows in large-scale experiments.

• Resource Efficiency: INDIGO resin’s durability allows for multiple reuse cycles, simplifying protocols and lowering costs without losing binding capacity.