Zymo Research - DirectDetect eliminates the need for RNA extraction in COVID-19 testing workflows
DNA/RNA Shield DirectDetect allows to go directly to PCR
DNA/RNA Shield DirectDetect is a reagent that allows users to go from sample collection directly to PCR. DNA/RNA Shield DirectDetect safeguards viral genomes (nucleic acids) from degradation and provides ambient temperature collection and transportation of samples without the need for nucleic acid extraction.*
*DNA/RNA Shield DirectDetect does not inactivate pathogens. For inactivating transport media, see DNA/RNA Shield.
DirectDetect in COVID-19 Testing - From Sample Collection Directly to PCR
Eliminates the need for RNA (or DNA) extraction, allowing for rapid, direct, cost-effective analysis of samples
No inhibition of real-time PCR
Reduces sample viscosity to minimize pipetting errors with automated liquid handlers
Zymo DirectDetect - No PCR Inhibition
Detection of SARS-CoV-2 from Swabs Collected into DirectDetect
Compared to other reagents used for collecting swab samples, such as saline, DirectDetectTM does not inhibit downstream real-time PCR.
Swabs were collected into either DirectDetectTM or 0.9% saline, and then spiked with heat-inactivated SARS-CoV-2 at a concentration of 5,000 copy/mL. 10μl of each sample was used as input into the Quick SARS-CoV-2 Multiplex Kit (R3013) for real-time PCR analysis