Single-cell DNA methylation sequencing is transforming how we read tumor epigenomes. This webinar covers practical and analytical approaches for profiling methylation heterogeneity at single-cell resolution in hormone-driven breast cancer. We also explore the role of ‚epimutations‘ and how they can be used to reconstruct lineage trees.
You Will Learn:
- How single-cell methylation profiling scales to thousands of cells, and what sample inputs and preparation it requires
- Analytical strategies for calling methylation states, defining cell populations, and integrating methylation with transcriptome and chromatin readouts
- How methylation heterogeneity tracks endocrine response, tumor evolution, and early transformation in breast cancer
Rewiring of regulatory elements is a key hallmark of breast cancer initiation and progression