ichorbio - Depletion & Validation Kits
Avoid the Epitope Masking Trap
Successful in vivo immune cell depletion studies depend not only on effective depletion, but also on reliable validation. A frequent challenge in these workflows is epitope masking: the depletion antibody remains bound to the target, preventing the flow cytometry antibody from binding properly. The result can be a false readout that suggests complete depletion, even when target cells are still present.
ichorbio Depletion & Validation Kits are designed to overcome this issue by pairing premium in vivo depletion antibodies with carefully selected, non-competing conjugated antibodies for accurate flow cytometry validation.
Why epitope masking matters
The challenge
In a depletion workflow, first a depleting antibody in vivo is administered and then the remaining cell population is analyzed by flow cytometry. Problems arise when the validation antibody recognizes the same or an overlapping epitope as the depletion antibody. This can lead to:
- blocked antibody binding during validation
- false-negative detection of residual cells
- incorrect interpretation of depletion efficiency
- compromised confidence in experimental results
In short: no signal does not always mean no cells.
The ichorbio solution
ichorbio addresses this problem with paired reagents in one validated kit. Each kit contains:
- an in vivo depletion antibody
- a non-competing conjugated primary antibody for flow cytometry validation. Because the validation antibody targets a different epitope, steric hindrance is minimized and flow cytometry readout becomes more reliable.
Key benefits
- Reliable validation: Matched antibody pairs help ensure that your flow cytometry results reflect true depletion rather than epitope interference.
- Simplified workflow: No more need to search literature or screen clones to identify compatible detection reagents
- Premium in vivo antibody quality: low endotoxin < 1 EU/mg, purity: >95%
- Validation conjugates are available as: PE, APC, FITC
