Large-Scale Validated Antibodies for Immunohistochemistry

Cancer & Normal tissues

MS Validated Antibodies (MSVA) is specialized in immunohistochemistry and provides a unique wealth of information for every antibody on the MSVA homepage, because the key to successful Immunohistochemistry is: Know your antibody!

Most of MSVA’s antibodies are rabbit recombinant antibodies. These share the advantage of complete reproducibility of their production because they are not generated by potentially unstable hybridoma cells but produced “in lab” based on the known DNA sequence of the antibody chains.

Key features & benefits: What differentiates MSVA from the rest of the Market?

  • Extremely sophisticated product selection – only 130 antibodies out of 4000 candidates were selected for the MSVA portfolio
  • Extensive validation process on tissues 
  • Unique documentation – images from more than 50 different normal tissues and more than 20 tumor images per antibody
  • All antibodies work exactly as described and visualized on the MSVA website 
  • Only 1 antibody per target to avoid confusion on what clone to select
  • Protocol recommendations (Manual Protocol, Dako, Leica & Ventana autostainers) 
  • Published data on the performance characteristics of MSVA antibodies on >10,000 tumors are compiled on the MSVA website 
    Already available for: PLAP, CPA1, Mesothelin, CK19, CK6, MUC5AC, Arginase-1, DOG-1
  • Extensive educational content is provided for every antibody including links to other relevant sites

The MSVA Validation Approach – Specificity testing

Recommended by the International Working Group for Antibody Validation (IWGAV)

1. Look up

Look up RNA data and the protein on the Human Protein Atlas (HPA) to see in what tissues we expect staining

2. Stain

Stain the antibody on a tissue microarray containing different normal tissues and different tumor types. See if staining matches HPA

3. Compare

We compare with independent clones for the same target to confirm or disconfirm staining. For the given example (PLAP MSVA candidate #2) the placenta staining is confirmed.

But other positive stainings are obviously non-specific. As the other antibody does not show them and RNA expression does not suggest these stainings.

We compare with an established clone (8A9) to confirm the results (see image beside). Again: Placenta staining is confirmed. And even more non-specificity for this PLAP antibody!

Decision: PLAP antibody „MSVA Product candidate #2” – discarded due to cross-reactivity“

Only excellent antibodies validated in more than a thousand tissues

Only one antibody per target, which performs at least as well as its “best in class” competitor

Unique documentation of antibody performance

Featured MSVA validated antibodies

PLAP (MSVA-350R)

Marker for germ cell tumors which can also be expressed in various types of adenocarcinomas. Details

CTLA-4 / CD152
(MSVA-152R)

Pivotal target in immune-oncology (e.g. Hodgkin’s lymphoma).
Details

Ki-67 (MSVA-267M)

Proliferation marker expressed by cells in G1, S, G2 and M phases of cell cycle.
Details

Carboxypeptidase A1 / CPA1 (MSVA-601M)

Protein expressed on normal and neoplastic pancreatic acinar cells.
Details