Navinci - NaveniFlex™ Kits for flexible PLA
Proximity Ligation Assay (PLA) optimized for cells or tissues: Detect proteins, protein-protein interactions, or protein modifications in tissue
Navinci’s NaveniFlex™ Kits are in situ proximity ligation assay (PLA) kits designed for the precise detection of proteins, protein-protein interactions, and post-translational modifications within cells and tissue samples. These kits utilize proprietary Navenibodies as secondary detection reagents, which, when in close proximity, initiate a rolling circle amplification, resulting in a quantifiable fluorescent or chromogenic signal. This technology enables researchers to visualize and quantify molecular interactions with high specificity and sensitivity, even for low-abundance targets, while preserving the spatial context of the sample.
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PLA Kits for Tissues:
PLA Kits for Cells & Tissues:
PLA Kits for Automation:
PLA Kits optimized for Cells
NaveniFlex™ Cell
The NaveniFlex™ Cell PLA is an in situ proximity ligation assay kit optimized for cell samples (FFPE and fresh formaldehyde-fixated cells). It enables in situ fluorescence visualization of proteins, protein – protein interactions, or protein modifications in cell samples. It allows to analyze even the most low-abundance protein targets with precision and ensures high specificity. The obtained data is quantifiable. High throughput screening with 96 well plate protocol is possible.
NaveniFlex™ Cell Workflow
Sample Types
NaveniFlex™ Cell Products
Naveni™ TriFlex Cell
Study protein functional states and interplay – Unique Product!
Naveni™TriFlex Cell is designed for fluorescence detection of two proteins in a free and complex state. Based on the Naveni™ Proximity Ligation Technology, the in situ kit provides accurate data with precise staining of total protein A, total protein B, and the interaction AB between the two proteins.
- Validated for cell samples
- To be used with an own primary mouse and rabbit antibody pair
- Very easy to perform (identically to an immunostaining)
- Compatible with regular immunostaining equipment (FITC, Cy3, Cy5 filters)
Naveni™ TriFlex Cell - Workflow and Sample Types
Naveni™ TriFlex relies on two user-determined primary antibodies against the targets of interest, and on proprietary TriFlex Navenibodies (in orange), which are antibody-based proximity reagents. Only proteins located at <40 nm distance are recorded as interacting. Naveni™ TriFlex detects total protein A (i.e., both free and in complex with B, yellow), total protein B (maroon), and the AB interaction (blue). The detected A, B and AB signals are amplified and generate fluorescent readout in three channels corresponding to each protein pool.
Naveni™ TriFlex Cell - 3 Application Examples
1- Protein interaction
β-catenin/ E-cadherin staining in MCF7 cells, 40x objective. β -catenin and E-cadherin are highly expressed proteins and the interaction between the two proteins is important for cell-cell adhesion.
2 - Co-localization is not a synonym to protein interaction
NaveniTMTriFlex Cell was used to simultaneously detect total COX1 and GM130, and their potential interaction. This assay was used as a biological negative control, as COX1 and GM130 belong to different cell compartments and thus do not interact, what is visualized using the Cy5 channel. Note that in the merged image, however, COX1 and GM130 appear partially co-localizing (white) where signals are particularly dense.
3 - From Birth to Death
NaveniTMTriFlex Cell was used to simultaneously detect free and interacting Lamin B1 and Histone H3, 40x objective, deconvolved.
Naveni™ TriFlex Cell Products
Naveni TriFlex Cell MR
Detect and quantify total protein A, total protein B, and the interaction AB between the two proteins. The assay is designed to be used with a mouse and rabbit primary antibody pair. Details
PLA Kits optimized for Tissues
NaveniFlex™ Tissue
NaveniFlex™ Tissue kits are specifically optimized for tissue samples (FFPE, frozen) to improve sensitivity and to overcome unspecific bindings and common background challenges. The kits deliver highly reproducible and accurate results in tissue samples, even for the least abundant protein interactions. Compatible with traditional immunostaining equipment (fluorescent readout).
EASY PROCEDURE: The workflow is basically identical to a traditional immunostaining!
- Detect protein-protein interactions or post-translational modifications
- Detect low abundant proteins in situ
- Analyze protein interplay in intact tissue morphology
- Visualize signals that would otherwise be obscured by background
NaveniFlex™ Tissue solves common tissue staining problems
PROBLEM:
A commercial in situ proximity ligation kit X (a, b) was used for Podocalyxin/Ezrin staining in human FFPE breast cancer tissue. The kit produces similar stain in both the positive (a) and the technical negative control (b) due to high background (hazy red staining) which obscures proximity signal.
SOLUTION:
NaveniFlex™ Tissue visualizes the Podocalyxin/Ezrin interactions clearly (discrete red specks), free of background (c), and leaves the technical negative control blank (d) in FFPE breast cancer tissue. A picture does say more than a thousand words!
The NaveniFlex™ Tissue kit includes two Navenibodies conjugated to proprietary oligo arms (orange antibodies). Only if the Navenibodies are in close proximity, they generate a rolling circle amplification reaction, leading to a strong and distinct dot that is easily quantifiable.
NaveniFlex™ Tissue Products
NaveniFlex Tissue MR Red
NaveniFlex Tissue MR Atto647N is compatible with a mouse and rabbit primary antibody pair and uses a TEX615 fluorophore for detection. Details
NaveniFlex Tissue MR Atto647N
NaveniFlex Tissue MR Atto647N is compatible with a mouse and rabbit primary antibody pair and uses a Atto647N fluorophore for detection. Details
PLA Kits optimized for Cells & Tissues
NaveniBright™ Kits
NaveniBright™ Products
NaveniBright HRP
The assay is designed to be used with a mouse and a rabbit primary pair. Chromogenic readout with HRP substrate. Details
PLA Kits for Automation
Naveni BOND RX Kits
Naveni BOND RX Products for automated PLA
NaveniBright™ BOND RX
HRP
Chromogenic Readout
The NaveniBright™ BOND RX HRP is a flexible method for in situ proximity ligation assay, using one mouse and one rabbit antibody on FFPE tissue or cell sections and featuring a chromogenic readout with any brightfield microscope. Optimized for Leica Biosystems‘ BOND RX/RXm instruments.
NaveniFlex™ BOND RX,
Red
Fluorescent Readout
Detect proteins, protein – protein interactions, or protein modifications with Naveni® in situ technology using BOND RX/RXm Fully Automated Research Stainer. The kit should be used with the user’s primary antibodies of choice (raised in mouse and rabbit). Detection with Texas Red fluorophore is included.
NaveniFlex™ BOND RX, Atto647N
Fluorescent Readout
NaveniFlex™ BOND RX/RXm is an automatable kit for in situ proximity ligation assays on FFPE tissue or cell sections with a fluorescent readout. It works with two unlabeled primary antibodies (mouse and rabbit) to detect either protein interactions or multiple sites on the same protein. Optimized for Leica Biosystems‘ BOND RX/RXm instruments.
