Navinci - Naveni® BOND Kits for automated PLA
Fully automated in situ proximity ligation assays on BOND RX/RXm Research Stainer (Leica Biosystems)
For the first time is proximity ligation assay available with an automated workflow. The NaveniBright™ and Naveni® PD1/PD-L1 BOND RX HRP introduces two novel products seamlessly integrating chromogenic readout automation on the BOND RX/RXm Fully Automated Research Stainer (Leica).
Naveni® kits for BOND RX/RXm
Time saving, robust and reproducible: Time-saving automation and streamlined workflows maximize research productivity enabling high throughput studies using proximity ligation assay. Automated assay workflows reduce hands-on time and minimize variability.
- Compatible with both, Leica BOND RX and BOND RXm
- Parallel runs of 30 slides
- Entire process takes 9-12 hours
- Standard template included in the Leica BOND Software
- Brightfield readout
- Validated on FFPE tissue, TMA and cell samples
- Compatible with mouse and rabbit primary antibodies
Workflow of Naveni BOND Kits
Flexible Kits for automated PLA
NaveniBright™ BOND RX HRP Kit
Chromogenic Readout
Fully automtated in situ kit for the detection of protein interactions, protein expression and post translation modifications.
The NaveniBright™ BOND RX HRP is a flexible method for in situ proximity ligation assay, using one mouse and one rabbit antibody on FFPE tissue or cell sections and featuring a chromogenic readout.
It is seamlessly integrated for chromogenic readout on the Leica BOND RX platform. The stained slides can be imaged with any brightfield microscope. The NaveniBright BOND kit contains reagents to stain 30 slides, at a maximum of 4 runs.
NaveniFlex BOND RX, Red | Atto647N
Fluorescent Readout
NaveniFlex™ BOND RX/RXm is an automatable kit for in situ proximity ligation assays on FFPE tissue or cell sections with a fluorescent readout. It works with two unlabeled primary antibodies (mouse and rabbit) to detect either protein interactions or multiple sites on the same protein.
The NaveniFlex™ protocol has been optimized for Leica Biosystems‘ BOND RX/RXm instruments, stained slides can be imaged using any fluorescence microscope or scanner with the appropriate filter set (DAPI, FITC, Cy3, TexasRed, Cy5). The kit provides reagents for 30 slides over a maximum of 4 runs, with two available fluorophores for the readout.
Target-specific Kits for automated PLA
Naveni PD1/PD-L1
BOND RX HRP
Chromogenic Readout
Naveni PD1/PD-L1 BOND RX HRP is a fully automtatedin situ kit for precise visualization of the interaction of PD1/PD-L1 within the tissue microenvironment. Naveni PD1/PD-L1 BOND RX HRP is seamlessly integrated for chromogenic readout on the Leica BOND RX platform. Naveni PD1/PD-L1 BOND RX HRP is based on the cutting-edge Naveni® in situ proximity ligation technology. More
Naveni PD1/PDL1
BOND RX RED
Fluorescent Readout
Naveni PD1/PD-L1 BOND RX RED is a fully automtatedin situ kit for precise visualization of the interaction of PD1/PD-L1 within the tissue microenvironment. Naveni PD1/PD-L1 BOND RX RED is seamlessly integrated for Fluorescence readout (filterset suitable for Cy3,5) on the Leica BOND RX platform. Naveni PD1/PD-L1 BOND RX RED is based on the cutting-edge Naveni® in situ proximity ligation technology. More
Naveni PD1/PDL1
BOND RX Atto647N
Fluorescent Readout
Naveni PD1/PD-L1 BOND RX Atto647N is a fully automtatedin situ kit for precise visualization of the interaction of PD1/PD-L1 within the tissue microenvironment. Naveni PD1/PD-L1 BOND RX Atto647N is seamlessly integrated for Fluorescence readout (filterset suitable for Cy3,5) on the Leica BOND RX platform. Naveni PD1/PD-L1 BOND RX Atto647N is based on the cutting-edge Naveni® in situ proximity ligation technology. More
Applications: Protein Interactions, Modifications and Localization
Protein Interactions
Protein Modifications
Protein Localization
Precise Visualization of PD1/PD-L1 Interactions using Naveni® PD1/PD-L1 BOND RX
The Naveni® PD1/PD-L1 BOND RX HRP offer a comprehensive solution for studying PD1/PD-L1 interactions within the tissue microenvironment. With precise visualization capabilities, deeper insights into the dynamics of immune checkpoint pathways can be gained, facilitating the development of targeted immunotherapies for cancer treatment.
Investigating Protein-Protein Interactions
Naveni® kits for BOND RX/RXm Fully Automated Research Stainer enable researchers to explore complex protein-protein interactions with high precision and accuracy using their own primary antibodies. By visualizing these interactions within the spatial context of cells and tissues, researchers can unravel intricate signaling networks and uncover novel therapeutic targets for various diseases.
An example is the interactions between CD8 and MHC-I. Understanding these interactions is vital for elucidating the role of cytotoxic T cells in immune surveillance and their involvement in diseases such as cancer and autoimmune disorders.
Understanding Protein Function and Signaling Pathways
With Naveni® kits for BOND RX/RXm, researchers can investigate protein function and signaling events within the tissue microenvironment, shedding light on key biological processes and uncovering new avenues for therapeutic intervention. An example is the dynamic interplay between E-Cadherin and Beta-catenin. Providing insights into cell adhesion and signaling pathways, crucial for understanding tissue organization and the progression of various diseases, including carcinoid tumors.
Staining of CD8/MHCI interaction in breast cancer using NaveniBright BOND RX HRP (A, B positive signals, C negative control)
Comparison Data
Automated vs Manual in situ Proximity Ligation assay
Specificity and background
Download the application note High-throughput fully automated in situ proximity ligation assays for spatial interactomics (PDF) for detailed information about the validation and beta test results.
Navinci in partnership with Leica Biosystems.
