Caprico Biotechnologies, Inc. (CBI)

Flow Cytometry, Immunophenotyping

Caprico Biotechnologies, Inc. (CBI) is an ISO 13485:2016 certified and GMP compliant manufacturing facility providing high-quality purified and fluorochrome-conjugated RUO anti-human antibodies used in flow cytometry applications suitable for academic and clinical research projects. CBI products are also useful for immunophenotyping.

Currently, CBI offers products derived from more than 100 clones and conjugated with 24 different type of conjugates.

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Currently available conjugates

Immunophenotyping

CBI offers products useful for immunophenotyping of normal, activated (infection), and abnormal (autoimmune diseases, cancer, etc) human immune cells through surface or intracellular staining. All CBI products are produced from well-known clones and their quality or staining performance is equivalent to the respective products of major competitors. 

T-cell clonality:

PNH Panel:

6 Color TBNK:

Regulatory T Cell:

Dendritic Cell:

CD7 |CD45 |TRBC1 |TCRγδ |CD2 |CD4 |CD5 |CD8   >> Read more

FLAER iFluor™488 |CD59 PE |CD45 PerCP |CD55 APC  >> Read more

CD3 FITC |CD4 PE-Cyanine7 |CD8 APC-Cyanine7 |(CD16+CD56) PE |CD45 PerCP-Cy5.5 |CD19 APC 

CD25 |CD127 |FoxP3

CD3 FITC |(CD16+CD56) PE |CD45 PerCP-Cy5.5 |CD4 PE-Cy7 |CD14 APC-Cy7 |CD19 APC 

Flow Cytometry analyses for T cells

Caprico Biotechnologies Flow Cytometry Data CD45, CD3+, CD4+, CD8+

Flow Cytometry analyses for B cells and Monocytes

Caprico Biotechnologies Flow Cytometry Data CD20, CD19, CD14

Typical representative comparative flow cytometry analyses
 

Fluorophores - High-performance fluorescent dyes: iFluor™ and mFluor™

A new generation of small molecule fluorophores such as Alexa Fluor, iFluor, and mFluor are rapidly replacing many traditional fluorophores, providing users with greater light stability and brighter fluorescence. Beside the standard dyes, CBI offers the high-performance fluorescent dyes iFluor™ and mFluor™ series. 

iFluor™ Fluorophores

iFluor™ fluorophores are optimized for strong fluorescence, high photostability and pH independence, and are available to span the full UV-visible-IR spectrum. iFluor™ 488 has spectral properties essentially identical to Alexa Fluor® 488. iFluor™ 488 conjugates are prepared using a highly purified single rhodamine isomer which significantly improves lot to lot consistency. iFluor™ 647 dyes are spectrally similar to Alexa Fluor® 647 and DyLight™ 650 dyes.

mFluor™ Fluorophores

mFluor™ fluorophores are developed specifically for flow cytometry applications, to enable optimal multicolor detection. These dyes show large Stokes shifts and excellent water solubility. mFluor™ dyes span the full UV-visible spectrum and are designed to maximize excitation efficiency with one of the major light sources in flow cytometers such as the violet laser (405 nm) or the blue laser (488 nm). They are excellent alternatives to the phycoprotein-based tandem dyes that are quite difficult to couple to antibodies or other biomolecules.

See comparison data

Caprico Biotechnologies Fluorophores
Caprico Biotechnologies Fluorophores

Overview of available Clones and Conjugations

Antibody Clone CHF Un-conjugated FITC PE PerCP APC Biotin PerCP-Cyanine 5.5 PE-CF594 PE-Cyanine 5 PE-Cyanine 7 APC-Cyanine 7 APC-iFluor 700 PE-iFluor 594 iFluor 488 iFluor 594 iFluor 647 iFluor 700 iFluor 750 mFluor 450 mFluor 500 mFluor 510 mFluor 540
CD1a OKT6 Order + + + + + + + +
CD2 OKT11 Order + + + + + + + + + +   +   +
CD3 UCHT1/OKT3/SK7 Order + + + + + + + + + + + +   + + + +   +
CD4 OKT4/SK3 Order + + + + + + + + + + + +   + + + +   + + +
CD5 OKT1/UCHT2 Order + + + + + + + + + + + + + +
CD7 124-1D1/4H9 Order + + + + + + + + + + + +   +   + + +
CD8 OKT8/SK1 Order + + + + + + + + + + + +   + + + +   + +   +
CD10 C-1A12 Order + + + +   +      
CD10 FR4D11 Order + + + + + + + + + +
CD11a TS1/22 Order + + +
CD11b OKM1 Order + + + + + + + + + +   +
CD11c 3.9 Order + + + + + +
CD13 WM15 Order +       + +   +   +     +
CD13 APN-1464 Order + +   + + + + + +   +
CD14 M5E2 Order + +     +           + +            
CD14 26ic Order + + + + + + + + + + +       +   + +   +
CD15 FUT4/815 Order + + + + + + + + + + +   +
CD16 3G8 Order + + + + + + + + + + + + + +   +
CD19 4G7 Order + + + + + + + + + + + + + + + +
CD20 2H7 Order + + + + + + + + + + +       + +
CD21 HB5 Order + +
CD22 MYG13 Order + + + + + + + +
CD23 HD50 Order + + + + + + + +
CD25 7G7B6 Order + + + + + + + + +  
CD29 TS2/16 Order + + + +
CD31 JC70 Order + +
CD32 IV.3 Order + + + + + +
CD32b 4F5 Order + +
CD33 WM53 Order + + + + + + + + + + + +
CD34 4H11 Order + + + + + + + + +
CD38 OKT10 Order + + + + + + + + + + + +   + +   +
CD39 CAT-13.0B10 Order + + +
CD40 G28-5 Order + +
CD44 Hermes-3 Order + + + +   +
CD45 F10-89-4 Order + + + + + + + + + + + + + + + + + + + + + +
CD45 CF-10H5 Order + +                                      
CD45 2D1 Order + +                       +              
CD45RA OTH-74D4 Order + + +   + +   + + +   +
CD45RO UCHL1 Order + + + + + + +
CD47 B6H12.2 Order + +
CD52 CF-1D12 Order + +
CD54 LB-2 Order + + + + + +
CD55 F4-29D9 Order + + + + +
CD56 MY31 Order + + + + + + + + + + + +
CD57 HNK-1 Order + + + + + + + +   +
CD58 TS2/9 Order + +   +
CD59 BRA-10G Order + + + + +
CD62L DREG56 Order + + + + + +
CD64 10.1 Order + + + + + + +
CD64 32.2 Order + + + + +
CD66b G10F5 Order + + +   + + +
CD68 KP1 Order + +   + +
CD71 OKT9 Order + + + + + + + +
CD73 AD2 Order + + + + +
CD74 PIN.1 Order + +
CD79b CB3-1 Order + + + + + +   + +   +
CD81 1D6 Order + + + + + +   +
CD83 HB15e Order + +
CD86 BU63 Order + + +
CD90 F15-42-1 Order + + + + + +
CD117 BA7.3C.9 Order + + + + + + + + + +   +
CD151 50-6 Order + +
CD154 5C8 Order +   +
CD163 GHI/61 Order + + +
CD166 3A6 Order + +
CD183 1C6 Order + + +
CD192 1D9 Order   +   + +
CD226 11A8 Order + +
CD235a GYPA280 Order + + + + +
CD276 DCN.70 Order +
CD326 HEA125 Order + + +     + +
p53 PAb1620 Order + +
CDw78 BAG-12G2 Order +   +  
HLA-DR L243 Order + + + + + + + + + + + + + +     +  
HLA-ABC W6/32 Order + + + +
HLA-DR,DP,DQ IVA12 Order + + +
EGRF 528 Order + + +
FLAER   Order   +     +  
TRBC1 JOVI.1 Order   + + +
IgM M-2E6 Order + + + + + + + +   +  
IgD IA6-2 Order + + + +   +  
Kappa TB28-2 Order + + + + +   +  
Lambda HP6054 Order + + + +
PD-1  6D5 Order + + +   +

 

Flow Cytometry Services

Caprico Biotechnologies, Inc.  offers a range of flow cytometry services including:

  • Pre-Clinical Flow Cytometry assay development and services
  • Cellular assays including characterization of cellular response to viruses, drugs, vaccines, and other entities
  • Development of potential biomarkers for diagnostic use
  • Companion diagnostics assay development for immunotherapies, cancer treatment regimes, and immunomonitoring

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