PAN-Biotech - Fetal Calf Serum-Free Cell Culture (FCS-Free)

Fetal Calf Serum (FCS, also known as Fetal Bovine Serum, or FBS) is a common supplement of animal cell culture media. However, moral and scientific concerns demonstrate the urgency to switch to an FCS-free medium. 

Using PAN-Biotech FCS-free cell culture system, … to replace FBS is easier than you thought! PAN-Biotech products allow a seamless transition to chemically defined, serum-free cell culture. Discover PAN-Biotech’s chemically defined FBS replacement and serum-free media and revolutionize cell culture!

Why choose our PAN-Biotech FCS-Replacement?

-PAN Biotech is the supplier with the most publications for FCS-free use!

- Customizable & chemically defined

- Incredible flexibility for various cell lines

- GMP-compliant manufacturing

- Perfect for cutting-edge research

- Ethical, compliant and sustainable cell culture

List of PAN-Biotech Products used for a Serum-Free Cell Culture System

Sort for the different PAN-Biotech products, cell types and species to choose your best solution (Source: FCS-free database).

ProductCell line/typeSpeciesAnimal freePhenol redPricing (CHF)
Panserin 401HEK-293 (RRID:CVCL_6F04)HumanNoYes Order / CHF
Panserin 401L929 cellsMouseNoYes Order / CHF
Panserin 401HepG2/C3A (Human hepatocellular carcinoma. (RRID:CVCL_1098) )HumanNoYes Order / CHF
Panserin 401HybridomasHumanNoYes Order / CHF
Panserin 401HybridomasMouseNoYes Order / CHF
Panserin 401HybridomasRatNoYes Order / CHF
Panserin 401CHO (Chinese Hamster Ovary) cell lineHamsterNoYes Order / CHF
Panserin 401Vero cellsAfrican green monkeyNoYes Order / CHF
Panserin 401AstrocytesHumanNoYes Order / CHF
Panserin 401HeLaHumanNoYes Order / CHF
Panserin 401BHK-21 (hamster kidney cell)HamsterNoYes Order / CHF
Panserin 401Monkey kidney cell (COS-7)African green monkeyNoYes Order / CHF
Panserin 401Fibroblasts (General)HumanNoYes Order / CHF
Panserin 401Fibroblasts (General)MouseNoYes Order / CHF
Panserin 401Melanocytes (General)HumanNoYes Order / CHF
Panserin 401Neural cells (General)RatNoYes Order / CHF
Panserin 401MSCs (mesenchymal stem cells)HumanNoYes Order / CHF
Panserin 401CD34+HumanNoYes Order / CHF
Panserin 401T cellsHumanNoYes Order / CHF
Panserin 401MNCs (mononuclear cells)HumanNoYes Order / CHF
Panserin 401BMC (Bone marrow cell)MouseNoYes Order / CHF
Panserin 401hESC-derived VSMCs (vascular smooth muscle cells)HumanNoYes Order / CHF
Panserin 401PBMC (peripheral blood mononuclear cells)HumanNoYes Order / CHF
Panserin 401Jurkat T lymphocyte cellsHumanNoYes Order / CHF
Panserin 401RAW 264.7(monocytic cell line)MouseNoYes Order / CHF
Panserin 401T lymphocytesHumanNoYes Order / CHF
Panserin 401BMM (bone marrow derived macrophages)MouseNoYes Order / CHF
Panserin 401Spiral ganglion neuronsRatNoYes Order / CHF
Panserin 401HUVECs (human umbilical vein endothelial cells)HumanNoYes Order / CHF
Panserin 401HEK293THumanNoYes Order / CHF
Panserin 401SHSY5Y (RRID:CVCL_0019)HumanNoYes Order / CHF
Panserin 411BM-MSCs (Bone marrow-derived mesenchymal stromal cells)HumanNoYes Order / CHF
Panserin 293AHEK-293 (RRID:CVCL_6F04)HumanNoYes Order / CHF
Panserin 293AHEK293THumanNoYes Order / CHF
Panserin 293SHEK-293 (RRID:CVCL_6F04)HumanNoYes Order / CHF
Panexin basicHematopoietic Stem and Progenitor CellsHumanNoNo Order / CHF
Panexin basicMDM (monocyte-derived macrophages)HumanNoNo Order / CHF
Panexin basicPBMC (peripheral blood mononuclear cells)HumanNoNo Order / CHF
Panexin basicJurkat T lymphocyte cellsHumanNoNo Order / CHF
Panexin basicHEK293THumanNoNo Order / CHF
Panexin basicTHP-1 ((RRID:CVCL_0006)HumanNoNo Order / CHF
Panexin NTAHEK-293 (RRID:CVCL_6F04)HumanNoNo Order / CHF
Panexin NTAPC-3 (Prostatic cancer cell line. RRID:CVCL_0035)HumanNoNo Order / CHF
Panexin NTAASCs (adipose tissue derived stem cells)HumanNoNo Order / CHF
Panexin NTASZ95 sebocytes (RRID:CVCL_9803)HumanNoNo Order / CHF
Panexin NTAMDCK (canine kidney cell)Canine (Dog)NoNo Order / CHF
Panexin NTAHeLaHumanNoNo Order / CHF
Panexin NTAHepatocytes (general)MouseNoNo Order / CHF
Panexin NTAHuman Bronchial Epithelial Cells (HBEpC)HumanNoNo Order / CHF
Panexin NTAFibroblasts (General)HumanNoNo Order / CHF
Panexin NTAFibroblasts (General)MouseNoNo Order / CHF
Panexin NTAMSCs (mesenchymal stem cells)RatNoNo Order / CHF
Panexin NTACHO (Chinese Hamster Ovary) CHO-K1HamsterNoNo Order / CHF
Panexin NTAHFF (Human Foreskin Fibroblasts)HumanNoNo Order / CHF
Panexin NTAMRC-5 (lung fibroblast)HumanNoNo Order / CHF
Panexin NTADCs (dendritic cells)MouseNoNo Order / CHF
Panexin NTACaco-2 cellsHumanNoNo Order / CHF
Panexin NTAMDM (monocyte-derived macrophages)HumanNoNo Order / CHF
Panexin NTAMIA PaCa-2 cellsHumanNoNo Order / CHF
Panexin NTAA549 cellsHumanNoNo Order / CHF
Panexin NTAOSCC (oral squamous cell carcinoma)HumanNoNo Order / CHF
Panexin NTAMDA-MB-231 (breast cancer cells,  RRID:CVCL_0062)HumanNoNo Order / CHF
Panexin NTAHUVECs (human umbilical vein endothelial cells)HumanNoNo Order / CHF
Panexin NTAHEK293THumanNoNo Order / CHF
Panexin CDL929 cellsMouseYesNo Order / CHF
Panexin CDPBMC (peripheral blood mononuclear cells)HumanYesNo Order / CHF
Panexin CDJurkat T lymphocyte cellsHumanYesNo Order / CHF
Panexin CDA549 cellsHumanYesNo Order / CHF
Panexin CDTHP-1 ((RRID:CVCL_0006)HumanYesNo Order / CHF
Panexin BMMRAW 264.7(monocytic cell line)MouseNoNo Order / CHF
Panexin BMMBMM (bone marrow derived macrophages)MouseNoNo Order / CHF
Powerstem MSC1UCMSCs (umbilical cord mesenchymal stem cells)HumanNoYes Order / CHF
Powerstem MSC1At-MSCs (adipose tissue derived mesenchymal stem cells)HumanNoYes Order / CHF
Powerstem MSC1BMC (Bone marrow cell)HumanNoYes Order / CHF
Panserin 413T cellsMouseNoYes Order / CHF
Panserin 413PBMC (peripheral blood mononuclear cells)HumanNoYes Order / CHF
SpodopanSF9 (Spodoptera frugiperda)Spodoptera frugiperda (fall armyworm)NoNo Order / CHF
SpodopanSF21 (Spodoptera frugiperda)Spodoptera frugiperda (fall armyworm)NoNo Order / CHF
SpodopanSchneider 2 (CVCL_Z232)Drosophila melanogaster (fruit fly)NoNo Order / CHF
Panserin 801iPSc (induced Pluripotent Stem Cells)HumanNoYes Order / CHF
Panserin 801KeratinocytesHumanNoYes Order / CHF
Panexin NTA pharma gradePC-3 (Prostatic cancer cell line. RRID:CVCL_0035)HumanNoNo Order / CHF
Panexin NTA pharma gradeSZ95 sebocytes (RRID:CVCL_9803)HumanNoNo Order / CHF
Panexin NTA pharma gradeHeLaHumanNoNo Order / CHF
Panexin NTA pharma gradeHepatocytes (general)MouseNoNo Order / CHF
Panexin NTA pharma gradeHuman Bronchial Epithelial Cells (HBEpC)HumanNoNo Order / CHF
Panexin NTA pharma gradeFibroblasts (General)HumanNoNo Order / CHF
Panexin NTA pharma gradeFibroblasts (General)MouseNoNo Order / CHF
Panexin NTA pharma gradeMSCs (mesenchymal stem cells)RatNoNo Order / CHF
Panexin NTA pharma gradeCHO (Chinese Hamster Ovary) CHO-K1HamsterNoNo Order / CHF
Panexin NTA pharma gradeHFF (Human Foreskin Fibroblasts)HumanNo Order / CHF
Panexin NTA pharma gradeMRC-5 (lung fibroblast)HumanNoNo Order / CHF
Panexin NTA pharma gradeCaco-2 cellsHumanNoNo Order / CHF
Panexin NTA pharma gradeMDM (monocyte-derived macrophages)HumanNoNo Order / CHF
Panexin NTA pharma gradeMIA PaCa-2 cellsHumanNoNo Order / CHF
Panexin NTA pharma gradeA549 cellsHumanNoNo Order / CHF
Panexin NTA pharma gradeMDA-MB-231 (breast cancer cells,  RRID:CVCL_0062)HumanNoNo Order / CHF
Panexin NTA pharma gradeHUVECs (human umbilical vein endothelial cells)HumanNoNo Order / CHF
Panexin NTA pharma gradeHEK293THumanNoNo Order / CHF
Endopan 300 SLHUVECs (human umbilical vein endothelial cells)HumanNoYes Order / CHF

Serum Replacement: Panexin

Easy handling, full reproducibility, worry-free transition!

Panexin is designed to replace or to reduce serum in the cell culture in a very simple manner. It is suitable for adherent and non-adherent cells. In most cases there is no need to change the basal medium. As Panexin is fully defined and contains no peptones or hydrolysates, lot testing is not necessary anymore. It also allows high reproducibility and simplified downstream process. Panexin contains no growth factors and enables defined proliferation and differentiation of stem cells. Read more

  • Serum replacement or medium supplement to increase the productivity in cell cultures (CHO, MDCK, Vero, Hybridoma etc.)
  • Avoid the exosomes or stimulatory effects of growth factors in serum
  • Prevent the overgrowth of the culture by fibroblasts in coculture or in highly differentiated epithelial primary cultures
  • Guarantee the reproducibility and sensitivity in cell-based in vitro assays.
  • Generally reduce the amount of serum due to ethical concerns, lot-to-lot variability or high costs
Transition to Serum-Free System
Serum vs Serum-free Comparison

Efficiency and growth stimulation of Panexin NTA in DMDM/F12 compared to FBS in different cell lines

Panexin basic

Panexin basic

Serum substitute for adherent and non-adherent cells. Cost-effective allrounder!

Panexin CD

Panexin CD

Chemically defined (CD) serum replacement. Available in GMP-compliant quality. For adherent and non-adherent cells. 

Panexin NTA

Panexin NTA

Serum substitute optimized for demanding adherent cell culture. < 2% animal components.

Panexin BMM

Panexin BMM

For the generation of macrophages from mouse bone marrow under serum-free conditions.

Serum-Free Media: Panserin, Endopan, Spodopan, PANcell, ISF-1 Medium for Hybridoma

Panserin 401


Serum-free Allround-Medium for adherent and non adherent cells. Contains no growth factors. Contains no attachment factors.

With some cell types a pre-treatment of vessels with gelatine, collagen, poly-D-lysine or fibronectin may support serum-free conditions.

Panserin 293A

Serum-free medium for HEK293 cells in adherent culture as a direct replacement. Details | Order

Panserin 293S

Serum-free medium optimized for HEK293 cells in suspension culture. Details | Order

Panserin 411

Serum-free medium for Insulin-dependent adherent and non adherent cells w (e.g. CHO). Details | Order

Panserin 413

Serum-free medium for lymphocytes and hybridoma cells. Details | Order

Panserin 701

Serum-free medium for lymphocytes from whole blood. Details | Order

Panserin 801

Serum-free medium that selectively supports the growth of human keratinocytes and concurrently prevents the overgrowth with fibroblasts. Details | Order

Endopan 300 SL


Serum-free medium for HUVEC and endothelial cells



Serum-free and protein-free medium for insect cells in suspension.

PANcell T

Pancell T

Chemically defined serum-free medium for  lymphocytes from full blood.

PANcell Hybridoma

Pancell Hybridoma

Serum-free and defined medium for ihybridoma cell lines.


ISF-1 media for hybridoma

Serum-free and defined culture medium for hybridoma cell lines.

Serum-Free Stem Cell Media: PowerStem MSC1

PowerStem MSC1


PowerStem MSC1 is an easy to use xeno-free medium for in vivo proliferation of human mesenchymal stem cells (hMSC). PowerStem MSC1 supports long-term growth of MSC and preserves their multi-lineage potential. In addition, MSC cultured in PowerStem MSC1 expands faster and shows a significant reduction in hematopoietic cell contamination at early passages compared to serum-based media. To differentiate the proliferated MSC into different cells types the relevant protocols and differentiation factors should be used.