SeqWell
NGS Tools for Optimized Workflows and Superior Results

Simplify, Scale, and Accelerate Your Sequencing Workflows

Outperform Illumina with SeqWell's Cutting-Edge Technology

SeqWell revolutionizes next-generation sequencing (NGS) workflows with scalable, efficient, and high-performance solutions. Compared to competitors like Illumina’s Nextera XT, SeqWell offers unmatched flexibility, faster preparation times, and superior data quality, empowering researchers to achieve more with less effort and cost.
 
Whether it’s microbial sequencing, CRISPR QC, or long-read workflows, SeqWell’s  solutions provide unmatched performance, efficiency, and reliability.

Why SeqWell?

  • Innovation: Advanced transposase technologies like hyperactive Tn5 and TnX enhance library prep performance.
  • Efficiency Redefined: True multiplexing and auto-normalization simplify NGS workflows.
  • Unparalleled Flexibility: Suitable for diverse applications, from microbial sequencing to whole-genome analysis.
  • Cost-Effective: Reduces costs, time, and waste without sacrificing quality.

SeqWell vs. Illumina (Nextera XT)

  SeqWell (LongPlex / ExpressPlex) Illumina (Nextera XT)
Workflow Speed 100-155 minutes (consolidated worksteps) Longer, multi-step processes
Normalization Auto-normalized workflows Requires precise manual input quantification
GC Bias Significantly reduced for better uniformity Higher variability across samples, inconsistencies
Cost Efficiency Lower plastics usage, fewer repeat steps Higher consumable and labor costs
Scalability Up to 384 samples per run Limited to smaller batch sizes

Introducing TnX: Next-Level Enzyme Engineering

SeqWell’s TnX enzyme is a game-changer addressing the limitations of traditional Tn5-based workflows. The enhanced performance of SeqWell’s enzymes translates to:

  • Reduced Bias, Better Data Quality: Improved GC and insertion-site profiles for more uniform sequencing reads with minimal variability and coverage with fewer gaps.

  • Enhanced Activity, Higher Efficiency: Higher specific activity ensures better library yield and conversion efficiency for faster, more reliable library preparation with fewer worksteps.

  • Unparalleled robustness and flexibility: Reliably performing in stringent buffers. For applications from microbial sequencing to complex whole-genome analysis.

TnX Enzyme Traditional Tn5 Enzyme
Insertion Bias Significantly reduced, more randomized insertion Moderate
GC Coverage Improved uniformity, Fewer gaps in regions of interest Good
Specific Activity Increased specific activity increased library yields Standard
Library Complexity Enhanced for diverse reads, convert more DNA into library Moderate
Reaction Conditions Robust across conditions, ability to formulate in stringent buffers Standard
TnX-Directed-Engineering

Combining sequence analysis, multi-parameter performance data, and machine learning for truly directed evolution.

Key Products

ExpressPlex™ 2.0
Library Prep Kit (TnX)

Fastest NGS Library Prep Available

Outperforms Illumina in speed, scalability, and uniformity

  • 100 min. for complete library prep
  • 30 min. hands-on time only
  • One-Step workflow
  • TnX Transposase minimizes insertional bias compared to Tn5-based kits 
  • Built-in Normalization
  • CDI indexing  
  • 96- or 384-well plate formats
  • High multiplexing capabilities
  • Automation-friendly
  • Ideal for Plasmids; Amplicons; Microbial WGS

LongPlex™
Long Fragment Multiplexing Kit

Long-Read Sequencing Workflows

Outperforms Illumina by reducing equipment needs and labor-intensive steps

  • < 2hrs for complete protocol
  • 30 min. hands-on time only
  • Simultaneous Fragmentation & Indexing
  • Generates 8–10 kb
  • Hyperactive Tn5 transposase
  • Up to 96 samples with combinatorial UDIs
  • Cost-Effective thanks to early sample pooling
  • Automation-friendly, but not required
  • Ideal for Microbial / Small genome WGS; Targeted Hybrid Capture; Metagenomics; Low Pass, large genome

plexWell®
Library Prep Kits

  • Pool-then-prep design
  • 190 min. for complete prep
  • 55 min. hands-on time
  • Built-in auto-normalization
  • Up to 384 samples
  • Reduced costs

purePlex™
DNA Library Prep Kit

  • Built-in normalization
  • 155 min. for complete prep
  • 45 min. hands-on time
  • Reduces GC bias
  • Up to 96 samples
  • High multiplexing capability

Tagify™ Loaded Transposases

Enable fast, convenient and consistent fragmentation and tagging of DNA with ready-to-use reagents.

Supporting customizable adapters and user-defined formats, they simplify workflows.