SeqWell
NGS Tools for Optimized Workflows and Superior Results
Simplify, Scale, and Accelerate Your Sequencing Workflows
Outperform Illumina with SeqWell's Cutting-Edge Technology
Why SeqWell?
- Innovation: Advanced transposase technologies like hyperactive Tn5 and TnX enhance library prep performance.
- Efficiency Redefined: True multiplexing and auto-normalization simplify NGS workflows.
- Unparalleled Flexibility: Suitable for diverse applications, from microbial sequencing to whole-genome analysis.
- Cost-Effective: Reduces costs, time, and waste without sacrificing quality.
SeqWell vs. Illumina (Nextera XT)
SeqWell (LongPlex / ExpressPlex) | Illumina (Nextera XT) | |
Workflow Speed | 100-155 minutes (consolidated worksteps) | Longer, multi-step processes |
Normalization | Auto-normalized workflows | Requires precise manual input quantification |
GC Bias | Significantly reduced for better uniformity | Higher variability across samples, inconsistencies |
Cost Efficiency | Lower plastics usage, fewer repeat steps | Higher consumable and labor costs |
Scalability | Up to 384 samples per run | Limited to smaller batch sizes |
Introducing TnX: Next-Level Enzyme Engineering
SeqWell’s TnX enzyme is a game-changer addressing the limitations of traditional Tn5-based workflows. The enhanced performance of SeqWell’s enzymes translates to:
Reduced Bias, Better Data Quality: Improved GC and insertion-site profiles for more uniform sequencing reads with minimal variability and coverage with fewer gaps.
Enhanced Activity, Higher Efficiency: Higher specific activity ensures better library yield and conversion efficiency for faster, more reliable library preparation with fewer worksteps.
Unparalleled robustness and flexibility: Reliably performing in stringent buffers. For applications from microbial sequencing to complex whole-genome analysis.
TnX Enzyme | Traditional Tn5 Enzyme | |
Insertion Bias | Significantly reduced, more randomized insertion | Moderate |
GC Coverage | Improved uniformity, Fewer gaps in regions of interest | Good |
Specific Activity | Increased specific activity increased library yields | Standard |
Library Complexity | Enhanced for diverse reads, convert more DNA into library | Moderate |
Reaction Conditions | Robust across conditions, ability to formulate in stringent buffers | Standard |

Combining sequence analysis, multi-parameter performance data, and machine learning for truly directed evolution.
Key Products
ExpressPlex™ 2.0
Library Prep Kit (TnX)
Fastest NGS Library Prep Available
Outperforms Illumina in speed, scalability, and uniformity
- 100 min. for complete library prep
- 30 min. hands-on time only
- One-Step workflow
- TnX Transposase minimizes insertional bias compared to Tn5-based kits
- Built-in Normalization
- CDI indexing
- 96- or 384-well plate formats
- High multiplexing capabilities
- Automation-friendly
- Ideal for Plasmids; Amplicons; Microbial WGS
LongPlex™
Long Fragment Multiplexing Kit
Long-Read Sequencing Workflows
Outperforms Illumina by reducing equipment needs and labor-intensive steps
- < 2hrs for complete protocol
- 30 min. hands-on time only
- Simultaneous Fragmentation & Indexing
- Generates 8–10 kb
- Hyperactive Tn5 transposase
- Up to 96 samples with combinatorial UDIs
- Cost-Effective thanks to early sample pooling
- Automation-friendly, but not required
- Ideal for Microbial / Small genome WGS; Targeted Hybrid Capture; Metagenomics; Low Pass, large genome