seqWell
Optimized NGS Workflows and Superior Results
Get your data faster with seqwell's streamlined NGS library prep workflows
Why seqWell?
- Innovation: Advanced transposase technologies like hyperactive Tn5 and TnX enhance library prep performance.
- Efficiency Redefined: True multiplexing and auto-normalization simplify NGS workflows.
- Unparalleled Flexibility: Suitable for diverse applications, from microbial sequencing to whole-genome analysis.
- Cost-Effective: Reduces costs, time, and waste without sacrificing quality.
seqWell vs. Illumina (Nextera XT)
| seqWell (LongPlex / ExpressPlex) | Illumina (Nextera XT) | |
| Workflow Speed | 100-155 minutes (consolidated worksteps) | Longer, multi-step processes |
| Normalization | Auto-normalized workflows | Requires precise manual input quantification |
| GC Bias | Significantly reduced for better uniformity | Higher variability across samples, inconsistencies |
| Cost Efficiency | Lower plastics usage, fewer repeat steps | Higher consumable and labor costs |
| Scalability | Up to 384 samples per run | Limited to smaller batch sizes |
TnX Transposase: New Next-Level Enzyme
Workflow Simplicity & Performance powered by TnX Transposase
seqWell’s TnX enzyme is a game-changer addressing the limitations of traditional Tn5-based workflows. The enhanced performance of seqWell’s enzymes translates to:
Reduced Bias, Better Data Quality: Improved GC and insertion-site profiles for more uniform sequencing reads with minimal variability and coverage with fewer gaps.
Enhanced Activity, Higher Efficiency: Higher specific activity ensures better library yield and conversion efficiency for faster, more reliable library preparation with fewer worksteps.
Unparalleled robustness and flexibility: Reliably performing in stringent buffers. For applications from microbial sequencing to complex whole-genome analysis.
| TnX Enzyme | Traditional Tn5 Enzyme | |
| Insertion Bias | Significantly reduced, more randomized insertion | Moderate |
| GC Coverage | Improved uniformity, Fewer gaps in regions of interest | Good |
| Specific Activity | Increased specific activity increased library yields | Standard |
| Library Complexity | Enhanced for diverse reads, convert more DNA into library | Moderate |
| Reaction Conditions | Robust across conditions, ability to formulate in stringent buffers | Standard |
Combining sequence analysis, multi-parameter performance data, and machine learning for truly directed evolution.
Shorten your time to data with scalable true multiplexing library preps
A true multiplexed solution for quickly preparing NGS libraries from hundreds to thousands of samples. Our simple and scalable tagmentation-based workflows provide far greater consistency across samples and fewer handling steps downstream.
- Performance: TnX for improved uniformity of coverage with increased library yield & complexity
- Simple Workflows: Streamlined, plate-based tagmentation workflows
- Scalability: Multiplex 1000’s of samples with ease using our kits.
- Normalization: Built-in sample normalization
seqWell Library Preparation Kits
ExpressPlex™ 2.0 Library Prep Kit (TnX™)
Fastest NGS Library Prep Available. Outperforms Illumina in speed, scalability, and uniformity.
One-Step workflow. Built-in Normalization. Automation-friendly.
- Transposase Enzyme: TnX™
- Ideal for: Plasmid and amplicon sequencing
- Samples: Amplicons (>350 bp), plasmids, colony PCR, RCA
- No. of Samples: 96/384/custom
- Time: 100 min. total, 30 min. hands-on time
- Input Mass: 1-40ng
- Indexing Method: CDI
- Batch Range: 8-96 or 384 (depending on protocol)
- No. of available Unique Index Combinations: <6144
- Paired-End Reads (Clusters) per Sample: ≤2 million
AgriPrep™ Library Prep Kit (TnX™)
Powered by ExpressPlex. Bred for NGS library prep workflow simplicity and throughput!
One-Step workflow. Auto-Normalization. Automation-friendly.
- Transposase Enzyme: TnX™
- Ideal for: Genotyping-by-sequencing (GBS). Low-pass sequencing. Skim sequencing.
- Samples: Plant & animal genomic DNA
- No. of Samples: 96
- Time: 100 min. total, 30 min. hands-on time
- Input Mass: 10-200ng working range. 20-100ng normalization range.
- Indexing Method: CDI
- Batch Range: 8-96
- No. of available Unique Index Combinations: <1536 (off-the-shelf) and <3072 (custom)
- Paired-End Reads (Clusters) per Sample: ≤50 million
MosaiX™ Library Preparation Kit (TnX™)
Workflow simplicity of tagmentation. Reduced insertion bias of TnX. Performance benefits of ligation.
Unmatched speed, efficiency, and library complexity.
- Transposase Enzyme: TnX™
- Ideal for: Human WGS, Human WES, Human germline target capture panels, Plant & animal WGS and target capture panels
- Samples: Genomic DNA
- No. of Samples: 24/96
- Time: 90 min. total, 35 min. hands-on time
- Input Mass: 50-100ng
- Indexing Method: Any tagmentation-compatible indexing or custom primers.
- Batch Range: 1-96
- No. of available Unique Index Combinations: N/A
- Paired-End Reads (Clusters) per Sample: ≤400 million
LongPlex™ Multiplexing Kit (Hyperactive Tn5)
Enzymatic method to simultaneously fragment & index genomic DNA for scalable PacBio HiFi sequencing
Outperforms Illumina by reducing equipment needs and labor-intensive steps.
- Transposase Enzyme: Hyperactive Tn5
- Ideal for: Long-read sequencing of plasmids, Microbial/small genome WGS, Targeted hybrid capture, Metagenomic sequencing, Low-pass, large genome.
- Samples: Genomic DNA
- No. of Samples: 96
- Time: 105-225 min. total, 40 min. hands-on time
- Input Mass: 150-100ng
- Indexing Method: UDI
- Batch Range: 1-24 (dependsing on protocol)
- No. of available Unique Index Combinations: 96
- Paired-End Reads (Clusters) per Sample: N/A
seqWell Tagify™ Reagent
Tagify™ i5 UMI Adapter-loaded Transposase
Enables simple, scalable, and reliable NGS library preparation that requires unique molecular identifier (UMI) addition.
- Supports GUIDE-seq2 off-target analysis and other UMI-based assays
- Quickly and reliably fragment and tag genomic DNA via loaded Tn5 transposase
- Sequence tag includes Illumina-compatible i5 barcode and UMIs
- Fully QC’d reagent for reproducible tagmentation
Tagify™ Custom Loaded Transposases
For tagmentation-based NGS library preparation for simple, scalable, and reliable gene editing QC analysis & more.
- Quickly and reliably fragment and tag genomic DNA via loaded transposase
- Load Tn5 or TnX transposase with customer-specified payload for maximum assay flexibility
- Fully QC’d loaded transposase for reproducible tagmentation with certificate of analysis available
- Targeted sequencing assays or CRISPR QC using methods such as ATAC-Seq, CHANGE-Seq, SHARE-Seq, TTIS-Seq, or RGen-Seq
PhiRx™ Indexed Control
PhiRx™ Indexed Control
PhiRx™ Indexed Control is an easy, drop-in spike-in control to maximize the performance of XLEAP-SBS chemistry on high-throughput Illumina platforms. By color-balancing both sequencing reads and barcodes, PhiRx improves signal uniformity, boosts data quality, and helps recover reads that would otherwise be lost as “undetermined.”
+ Optimizes index diversity for XLEAP sequencing
+ Seamless spike-in alternative to Illumina PhiX Control v3
+ Corrects color balance issues in low-diversity and small batch runs
PhiRx provides diversity in both index and insert reads and is supplied at the same size and price point as Illumina’s PhiX controls, making adoption straightforward for existing workflows.
