NGS Library Preparation
seqWell - Reveal the Hidden Savings with ExpressPlex® 2.0
Up to 80–90% savings with ExpressPlex® 2.0
ExpressPlex® 2.0 vs. Nextera® XT — A True All-In Comparison
ExpressPlex® 2.0 is a next-generation NGS library prep kit that simplifies and accelerates the process of turning DNA into sequencing-ready libraries — combining speed, automation, and cost-efficiency in one powerful solution.
At first glance, ExpressPlex® 2.0 may seem more expensive than Illumina® Nextera® XT. But when every tube, every tip, every plate, and every minute of hands-on time is counted, ExpressPlex® 2.0 delivers up to 80–90% savings over Nextera® XT.
Faster. Simpler. Cheaper. Greener.
💡 Where the ExpressPlex® 2.0 Savings Come From
Hidden savings
- Protocol Simplicity
- Labor Savings – up to 90%
- Time Savings – 65% faster workflow
- Consumable Savings – over 90%
- Total Cost Saving – up to 80%
Reduced Complexity
- One-step tagmentation & amplification – replaces 5 Nextera® steps
- Auto-normalization – eliminates manual quantification
- Pre-plated reagents – no reagent prep or pipetting errors
- Early pooling – fewer cleanup steps, faster sequencing-ready libraries
Sustainable by Design
ExpressPlex® 2.0 doesn’t just save time and money — it saves resources
- >90% fewer pipette tips
- >80% less plastic waste
- Lower reagent volumes
💸 Hidden Costs: ExpressPlex® 2.0 vs. Nextera® XT
seqWell ExpressPlex® 2.0
Kit cost = 95% of total costs
Nothing extra to buy. Everything’s included in the kit.
⇒ Hidden costs = only 5%
Illumina Nextera® XT
Kit cost = ~75% of total cost
To buy extra: Index primers, magnetic beads, quant reagents
⇒ Hidden costs = ~25% or more (labor, consumables, extras)
🧠 Efficiency That Scales With You
seqWell ExpressPlex® 2.0
Throughput (1 Cycler, 1 FTE/day): up to 3’072 libraries
Indexing Capacity: up to 6’144
Illumina Nextera® XT
Throughput (1 Cycler, 1 FTE/day): 96 libraries
Indexing Capacity: 384
⚖️ Full comparison
Protocol Simplicity - 65% faster Workflow
- Auto-normalization
- Index and Ready
- Reaction plates provided
- One-step tagmentation/amplification
- Early sample pooling
Labor Savings - up to 90%
- Auto-normalization
- Pre-plated reagents
- Fewer sample touchpoints
- Early pooling
on high-throughput library prep
Consumables Savings - over 90%
- Early pooling
- Pre-plated reagents
- ‚Think Green’ protocols address sustainabilty
Time Savings
- Rapid protocol
- Automation-friendly
- 96-well or 384-well format
- Runs simultaneous workflows
- Higher multiplexing potential
Total Cost Savings - up to 80%
- All reagents included in kit
- Lower consumables cost
- Less labor required
