Protein G Resin FF Prepacked Column
|Catalog Number||Pack Size||List Price*||Quantity|
|L00681-12||1ml x 2||480,00||Add|
|L00681-51||5ml x 1||820,00||Add|
|L00681-55||5ml x 5||3'673,00||Add|
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GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF Prepacked Column.Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses
|Storage Conditions||Store in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.|
|Tech. Data Sheet||View datasheet|
|Link To Supplier||http://www.genscript.com|
|Material Safety Data Sheet||Download PDF|