10x Buffer R; Strep-Tactin Regeneration Buffer with HABA
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Buffer R is a Strep-Tactin Regeneration Buffer with HABA for the regeneration of immobilized Strep-Tactin . HABA (2-[4'-hydroxy-benzeneazo]benzoic acid) displaces desthiobiotin (present in Buffer E: Strep-Tactin Elution Buffer with Desthiobiotin) at the biotin-binding site of Strep-Tactin . The yellow HABA solution turns red upon binding to Strep-Tactin clearly indicating the regeneration process and activity status of the column. Visualization of column regeneration and activity status by color change. Do not use for regeneration of Strep-Tactin XT resin!
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