ATG8, Autophagy-related protein
Catalog Number | Pack Size | List Price* | Quantity | |
---|---|---|---|---|
AS14 2769 | 50 µl | 417,00 | Add |
* CHF, excl. 8.1% VAT and shipping costs
Product Description
For Arabidopsis thaliana the signal obtained using ATG8 antibodies is cleaner in case of roots compare to leaf material. For best results please follow extraction protocol described in lvarez et al. (2012). ATG8 signal corresponds to the two bands of 17 kDa.Preparation of a cell extract from Arabidopsis thaliana:A. Plants were first subjected to autophagy activating conditions: nutrient (nitrogen or carbon) limitation or oxidative stress in order to activate this degradative process.B. Total protein extracts can be obtained as described by lvarez. Leaves are grinded in liquid nitrogen with a minimal volume of extraction buffer (100 mM Tris-HCl pH 8, 400 mM sucrose, 1 mM EDTA, 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mg/ml sodium deoxycholate, 10 g/ml of leupeptin, 10 g/ml of pepstatin A, 4% (v/v) protease inhibitor cocktail from Roche).C. Cell debris is removed by centrifuging at 500 g for 10 min at 4 C.Important note:It is recommendable to use bigger gels in order to get a better resolution of ATG8 bands. Midi-protean gels are better than mini-gels. There are 9 ATG8 isoforms and this antibody will likely recognizes all of them.For immunolocalization protocol, please inquire.
Category | Antibody |
Supplier | Agrisera |
Application | Immunolocalization (IL), WB |
Regulatory Status | RUO |
Research Field | Plant Biology |
Species Specificity | Plant |
Immunogen | Fragment of recombinant ATG8 from Chlamydomonas reinhardtii, UniProt: A8JB85 |
Host | Rb |
Crossreactivity | Plant |
Clonality | Pab |
Format | Lyophilized |
Working Dilution | 1 : 1000 (IL), 1 : 1000-1 : 2000 (WB) |
Purity | Serum |
Storage Conditions | Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them ... |
References | Antibodies cited in scientific publications |
Tech. Data Sheet | View datasheet |
Link To Supplier | https://www.agrisera.com |
Material Safety Data Sheet | Download PDF |