Solis Hot Start HiFi PCR - Optimized version!
Confident amplification for challenging templates
- High-fidelity amplification (>100x Taq) and sequencing
- dUTP incorporation
- Damaged DNA (FFPE samples)
- Epigenetic and methylation studies
- AT- and GC rich templates
- Carryover contamination prevention (Kit Format)
Two formats — same HiFi performance
DNA Polymerase Kit:
Separate reagents for maximum flexibility and self-optimization
Master Mix:
Convenient pre-mix for fast setup and optimized performance
Solis BioDyne - Reagents for PCR, qPCR, RT-qPCR and cDNA
Solis BioDyne product range include all around DNA polymerases, PCR Master Mixes, dye-based qPCR mixes, probed-based qPCR mixes, , reverse transcription for cDNA synthesis, isothermal amplification reagents
All products are stable at room temperature and are shipped without ice!
Endpoint PCR - Standard & Hot Start
Full range of endpoint PCR reagents
Solis BioDyne provides endpoint PCR enzymes and master mixes for highly processive, thermostable, and robust amplification—from routine PCR to challenging templates and multiplex assays. Powered by Stability TAG technology, many enzymes and mixes offer enhanced ambient-temperature stability with no activity loss for up to 1 month, supporting easier handling and logistics. For fast gel workflows, Ready-to-Load (RTL) formats include two tracking dyes, enabling direct loading on agarose gels without adding separate loading dye. Read more
Standard endpoint PCR
enzymes & master mixes
For everyday PCR with reliable yield and robustness (classic Taq-based polymerases and convenient master mixes).
Hot-start PCR
enzymes & master mixes
For higher specificity and cleaner bands by minimizing non-specific amplification during reaction setup.
GC-rich PCR mixes
Formulated to improve amplification of GC-rich templates that are difficult to denature/extend.
Multiplex PCR mixes
Optimized for simultaneous amplification of multiple targets in one reaction (high-performance multiplexing).
Fast / ultra-fast endpoint PCR mixes
Designed to reduce cycling time while maintaining robust detection.
New! SolisAcura Format
UNG / carryover prevention mixes
Endpoint PCR mixes compatible with UNG (dUTP/UNG) workflows to reduce carryover contamination risk.
Ready-to-Load (RTL) formats
PCR master mixes that include tracking dyes for direct gel loading (saves time, fewer pipetting steps).
One-step RT-PCR (endpoint)
For cDNA synthesis + endpoint PCR in a single tube (RNA-to-PCR workflow; available in RTL versions for gels).
High-fidelity (proofreading) PCR
For higher accuracy applications (proofreading activity; useful for cloning, NGS prep, variant work).
New! Solis Hot Start HiFi Kit or Mix
qPCR and One-Step RT-qPCR
Optimized ready-to-use solutions for real-time PCR
Solis BioDyne’s qPCR and one-step RT-qPCR mixes are optimized, ready-to-use solutions for real-time quantification with all essential components included—supporting fast setup and reproducible performance. Choose between probe-based qPCR mixes (for higher specificity and multiplexing) and dye-based mixes using EvaGreen® or SolisGreen® for sensitive, cost-effective detection on most common real-time cyclers. For RNA workflows, one-step RT-qPCR kits combine reverse transcription and qPCR in a single tube to save time and reduce handling. Solis also provides a qPCR Mix selection tool to help select the best mix for your instrument and assay needs. Read more
Probe-based qPCR
Standard qPCR
Reliable real-time qPCR with hydrolysis probes (e.g., TaqMan®) for high specificity.
Multiplexing qPCR
Multiplexing-ready probe qPCR mixes to quantify multiple targets in one reaction (clean separation)
Fast & ultra-fast qPCR
For shorter run times with highly sensitive probe detection—suited to fast cycling protocols.
One-step RT-qPCR
Reverse transcription + probe-based qPCR in one tube for highly specific RNA quantification (e.g., SOLIScript® 1-step Probe Kit).
Dye-based qPCR
EvaGreen®
Intercalating dye chemistry for cost-efficient, sensitive quantification and straightforward assay design.
SolisGreen®
Highly sensitive dye-based detection (SYBR® Green I–like spectra) compatible with most major cyclers.
One-step RT-qPCR
Reverse transcription + dye-based qPCR in one tube for fast, high-throughput RNA analysis (e.g., SOLIScript® 1-step SolisGreen® Kit 2.0).
NEW!
Glycerol-free qPCR solution for effective lyophilization
- The kit consists of 2 components: a glycerol-free lyo-compatible qPCR mix and a lyophilization excipient mix
- Enables sensitive and specific DNA detection
- Contains dUTPs and UNG to prevent carryover contamination
- Compatible with fast cycling – results in 30 minutes
- Performance preserved after lyophilization
cDNA synthesis, 1-step RT-PCR Kit and RNase Inhibitors
Solis BioDyne offers thermostable reverse transcriptases, ready-to-use cDNA synthesis kits/mixes, one-step RT-PCR kits, and RNase inhibitors for fast and reliable RNA workflows. The RT enzymes support a wide reaction temperature range (37°C–60°C) to help overcome RNA secondary structures, and workflows can be completed quickly (from ~15 minutes depending on kit/assay). Many cDNA synthesis kits and mixes are designed for ice-free handling, with room-temperature stability for up to 30 days—ideal for convenient setup and simplified logistics. A Product Selection Guide helps users choose the right solution for their priming strategy and application. Read more
Reverse Transcriptases (RT)
Standalone RT enzymes for first-strand cDNA synthesis; thermostable performance up to 60°C supports challenging RNA templates.
FIREScript®
Powerful genetically modified MMLV-basedreverse transcriptase
SOLIScript®
in silico-designed reverse transcriptase that suits best with oligo dT and gene-specific primers.
First-strand cDNA synthesis
Fast, robust first-strand cDNA synthesis with thermostable reverse transcriptases, ice-free setup options, and convenient mix or flexible kit formats for routine to challenging RNA templates.
cDNA Synthesis Kits (flexible kit format)
Powerful genetically modified MMLV-basedreverse transcriptase
cDNA Synthesis Mixes
(convenient all-inclusive format)
Ready-to-use mixes designed to minimize pipetting and speed up setup; suitable for routine high-throughput cDNA synthesis.
1-step RT-PCR Kits (endpoint RT-PCR)
Sensitive and highly specific cDNA synthesis and end-point PCR in a single tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.
Available in fast formats and with contamination control options (e.g., UNG) depending on kit.
RNase Inhibitors - RiboGrip®
Protect RNA integrity during reverse transcription and handling steps (often included in mixes/kits as an integrated workflow safeguard).
Illumina highlights RiboGrip® as the preferred RNase inhibitor in their official training materials for Single Cell 3’ RNA Prep.
Additional reagents required in PCR and post-PCR analysis
Solis BioDyne provides the essential “support reagents” you need to run PCR smoothly and analyze products confidently—covering DNA ladders, nucleotides, loading dyes, MgCl₂ and PCR enhancers, plus enzymes for MALDI-TOF workflows. These add-ons help you optimize reaction performance, streamline gel analysis, and support downstream applications where consistent quality matters.
- DNA Ladders, Nucleotides (Set or Mix), DNA Loading Dyes
- MgCl2 and PCR enhancer
- Enzymes for MALDI-TOF
DNA Ladders
Ready-to-use size markers for quick DNA fragment sizing on agarose gels.
Nucleotides (dNTP Set or Mix)
High-purity dNTPs supplied as individual sets or convenient mixes for PCR and related enzymatic reactions.
DNA Loading Dyes
Loading dye buffers (incl. tracking dyes) for direct gel loading and improved sample handling.
MgCl₂ (PCR optimization)
Stock MgCl₂ solutions for fine-tuning Mg²⁺ concentration to improve yield, specificity, and robustness.
PCR Enhancer
Additives to improve amplification of difficult templates and increase overall PCR performance.
Enzymes for MALDI-TOF
Enzymes supporting MALDI-TOF assay workflows and related downstream processing steps.