Solis BioDyne - Reagents for PCR, qPCR, RT-qPCR and cDNA
Solis BioDyne product range include all around DNA polymerases, PCR Master Mixes, dye-based qPCR mixes, probed-based qPCR mixes, , reverse transcription for cDNA synthesis, isothermal amplification reagents
All products are stable at room temperature and are shipped without ice!
Solis BioDyne Product Categories
Endpoint PCR
Full range of endpoint PCR reagents
- Hot-start and standard DNA polymerases with buffers
- Convenient Master Mixes for GC-rich and multiplex reactions
- Master Mixes with Ready-to-Load feature enable direct loading to gel
qPCR and RT-qPCR
Optimized ready-to-use solutions for real-time PCR
- Probe-based qPCR Mixes for standard, fast and ultra fast applications
- Dye-based qPCR Mixes with EvaGreen® and SolisGreen® dyes
- Time saving one-step RT-qPCR kits for quick RNA analysis
cDNA Synthesis
Reverse transcriptases (RT) for first-strand cDNA synthesis
- RT enzymes with wide range of reaction temperature: 37ºC – 60ºC
- Fast reaction time – from 15 minutes
- Available in convenient mix and flexible kit format
Additional Enzymes and Reagents
Common reagents required in PCR and post-PCR analysis
- DNA Ladders
- Nucleotides in Set and Mix format
- DNA Loading Dyes
- MgCl2 and PCR enhancer
- Enzymes for MALDI-TOF
Standard and Hot Start Endpoint PCR
Solis BioDyne offers complete range of enzymes and master mixes for highly processive, thermostable and robust PCR reactions. Due to Stability TAG technology, all enzymes and master mixes are stable at ambient temperature with no activity loss up to 1 month. Read more
The Ready to Load (RTL) format includes two tracking dyes for direct loading on gel.
Standard endpoint PCR
Highly processive, thermostable Taq DNA polymerase
Convenient Master Mix for standard PCR applications, based on FIREPol® DNA Polymerase. Available as RTL format.
Hot-Start endpoint PCR
Hot-start DNA Polymerase for your everyday PCR needs.
Hot-start Master Mix designed to provide highly specific high-yield amplification of GC-rich templates.
High performance hot-start Master Mix for efficient multiplex reaction – up to 18 targets! Available as RTL format.
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions. Available as RTL format.
Ultra fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Available as RTL format.
Ultra fast endpoint PCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target detection. Suitable for UNG treatment to prevent carryover contamination. Available as RTL format.
qPCR and One-Step RT-qPCR
Solis BioDyne’s qPCR and one-step RT-qPCR kits are optimized ready-to-use solutions for real-time qPCR assays with all the components necessary to perform qPCR. Solis BioDyne has mixes for both – probe based approach and intercalating dye-based approach (highly sensitive EvaGreen® or SolisGreen® dye). Find suitable mix for your qPCR platform from the qPCR Mix selection tool or product list below. Read more
Dye-based qPCR
EvaGreen® shows a higher fluorescence level than SYBR Green, as well as high sensitivity for detecting low template concentrations:
Ready-to-use solution for simple dye-based qPCR assays, incorporating EvaGreen® dye. Available for ROX, no ROX, and capillary cyclers.
Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
Optimized ready-to-use solution for High-Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. Allows detection of DNA sequence variations, single nucleotide polymorphisms (SNP genotyping) and genetic mutations. Compatible with most cyclers.
SolisGreen® is a bright and sensitive dye ideal for low DNA concentrations. Suitable for commercial applications:
Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets. Compatible with most qPCR platforms.
SolisFAST® combined with SolisGreen® allows extremely fast and highly sensitive dye-based qPCR for most demanding applications:
Extremely fast and highly sensitive dye-based qPCR that includes novel in-silico designed SolisFAST® DNA Polymerase Mix for most demanding applications.
Probe-based qPCR
Sensitive qPCR Mix designed for effective quantification of DNA targets with probe-based chemistry.
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
High-performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates.
SolisFAST® allows for ultra-fast probe-based qPCR:
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification.
Ultra-fast probe-based qPCR Master Mix that includes novel in-silico designed SolisFAST® DNA Polymerase for robust and accurate target quantification. Suitable for UNG treatment to prevent carryover contamination.
One-Step RT-qPCR
Solis BioDyne’s one-step RT-qPCR kits provide a ready-to-use solution with all the components necessary for sensitive and highly specific cDNA synthesis and qPCR (probe- or dye-based) in a single tube. Check the cycler compatibility.
cDNA synthesis and dye-based qPCR:
Highly sensitive cDNA synthesis and dye-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
cDNA synthesis and probe-based qPCR:
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
NEW!
Convenient single vial format and sensitive 5-plex amplification for demanding one-step RT-qPCR experiments. Includes UNG enzyme to prevent carryover contamination and false positive results. High multiplexing capacity and inhibitor tolerance.
SOLIScript® Fast 1-step RT-qPCR Mix with UNG enables increased throughput with 5-plex amplification. Five-plex RT-qPCR reactions (FAM, blue HEX, green; ROX, red; Cy5, purple; Cy5.5, orange) with SOLIScript® Fast 1-step RT-qPCR Mix with UNG show strong amplification and consistent efficiencies across all five targets. Reactions were performed on the Bio-Rad CFX96 platform with six 10-fold dilutions of a reference human total RNA (from 1000 ng/µl to 0.01 ng/µl).
cDNA synthesis
Solis BioDyne cDNA synthesis Kits and Mixes are all stable at room temperature for 30 days. Use convenient ice-free reaction set-up, wide reaction temperature range and fast reaction time to get excellent results! Check out the Product Selection Guide.
Reverse Transcriptases (RT)
Powerful genetically modified MMLV-based FIREScript® reverse transcriptase with increased thermostability and exceptional stability at room temperature. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
Unique in silico-designed SOLIScript® reverse transcriptase with high thermostability and exceptional stability at room temperature. SOLIScript® suits best with oligo dT and gene-specific primers. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
First-strand cDNA synthesis
Individual components:
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis.
A flexible and highly specific cDNA synthesis kit that contains all the reagents you need for first-strand cDNA synthesis. Includes oligo dT primers.
Convenient pre-mixes:
Convenient all-inclusive product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip™) and a reaction premix in separate tubes. Primers not included.
Convenient all-inclusive product format for first-strand cDNA synthesis using gene-specific primers. A combined enzyme mix (SOLIScript® and RiboGrip™) and a reaction premix in separate tubes. Primers not included.
RNase Inhibitor
RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor.
Additional reagents required in PCR and post-PCR analysis
DNA Ladders
1kb DNA ladder with 13 discrete bands ranging from 250 bp to 10’000 bp. Ready to load format (RTL).
100bp DNA ladder containing 13 discrete bands ranging from 100 bp to 3000 bp. Ready to load format (RTL).
Loading Dyes
Comigrate with 300 bp and 3500 bp fragments (in 1x TBE) or 400 bp and 5000 bp fragments (in 1x TAE) on 1% agarose gel.
Comigrate with 30 bp and 3500 bp fragments (in 1x TBE) or 60 bp and 5000 bp fragments (in 1x TAE) on 1% agarose gel.
Comigrates with 300 bp fragments (in 1x TBE) or 400 bp fragments (in 1x TAE) on 1% agarose gel.
Comigrates with 40 bp fragments (in 1x TBE) or 60 bp fragments (in 1x TAE) on 1% agarose gel.
Enzymes for MALDI-TOF
Assay success rate in MALDI-TOF is 99% !
DNA Polymerase with enhanced efficiency for incorporating unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides.
Hot-start DNA Polymerase with enhanced efficiency for incorporating unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides.
Further reagents
Unique heat-labile inhibitor tolerant Uracil-N-Glycosylase. Ideal for carryover contamination prevention.
Additive to enhance amplification of GC-rich sequences.
Additive for adjusting Mg2+ concentration in PCR assay.
