Solis BioDyne - Reagents for PCR, qPCR, RT-qPCR and cDNA
Solis BioDyne is a trusted trademark worldwide. Their product range include all around PCR, qPCR and RT-qPCR: DNA polymerases, endpoint PCR master mixes, dye-based qPCR mixes, probed-based qPCR mixes, reverse transcription reagents for
Good to know:
All products are stable at room temperature and are shipped without ice!
Endpoint PCR
Solis BioDyne offers complete range of enzymes and master mixes for highly processive, thermostable and robust PCR reactions. Due to Stability TAG technology, all enzymes and master mixes have an enhanced stability at ambient temperature with no activity loss up to 1 month.
Standard PCR
The Ready to Load (RTL) format includes two tracking dyes for direct loading on gel.
Hot-Start PCR
SolisFAST® productline feature ultrafast and remarkably PCR inhibitor tolerant reactions:
Dye-based qPCR | Probe-based qPCR | High Resolution Melt (HRM)
Solis BioDyne’s quantitative PCR kits are optimized ready-to-use solutions for real-time qPCR assays (dye-based or probe-based) with all the components necessary. Check for cycler compatibility.
Dye-based qPCR
EvaGreen® shows a higher fluorescence level than SYBR Green, as well as high sensitivity for detecting low template concentrations:
SolisGreen® is a bright and sensitive dye ideal for low DNA concentrations. Suitable for commercial applications:
SolisFAST® combined with SolisGreen® allows extremely fast and highly sensitive dye-based qPCR for most demanding applications:
Probe-based qPCR
„Universal“ is a high-performing probe-based qPCR Mix for AT-rich, regular and GC-rich templates:
SolisFAST® allows for ultra-fast probe-based qPCR:
Use SolisFAST® Probe qPCR Mix with UNG treatment to prevent carryover contamination:
High Resolution Melt (HRM)
Genotyping of a C insertion in BRCA1 gene. Green lines represent wildtypes without an insertion, red lines represent a C insertion and blue line represents a patient with unknown phenotype.
Ready-to-use 5x-concentrated optimized ready-to-use solution for High Resolution Melt (HRM) analysis incorporating the highly sensitive EvaGreen® dye. Excellent resolution in HRM assays allows detection of DNA sequence variations, single nucleotide polymorphisms (SNP genotyping) and genetic mutations. Compatible with most cyclers.
One-Step RT-qPCR
Solis BioDyne’s one-step RT-qPCR kits provide a ready-to-use solution with all the components necessary for sensitive and highly specific cDNA synthesis and qPCR (probe- or dye-based) in a single tube. Check the cycler compatibility.
One-Step RT-qPCR
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube:
Highly sensitive cDNA synthesis and dye-based qPCR in a single tube:
cDNA synthesis
Solis BioDyne cDNA synthesis Kits and Mixes are all stable at room temperature for 30 days. Use convenient ice-free reaction set-up, wide reaction temperature range and fast reaction time to get excellent results! Check out the Product Selection Guide.
Reverse Transcriptases (RT)
First-strand cDNA synthesis
All the separated reagents you need for cDNA synthesis are contained in this kit:
Convenient pre-mix for fast and robust cDNA synthesis:
RNase Inhibitor
Novel in-silico engineered, protein-based RNase inhibitor:
Enzymes for MALDI-TOF
TERMIPol® & HOT TERMIPol® DNA Polymerase
TERMIPol® and HOT TERMIPol® DNA Polymerase are thermostable DNA polymerases that efficiently incorporate unconventional nucleotides such as ddNTPs, acyNTPs or fluorescent nucleotides. Assay success rate in MALDI-TOF is 99% !
Common reagents required in PCR and post-PCR analysis
Nucleotides (dNTPs)
DNA Ladders
1kb DNA ladder with 13 discrete bands ranging from 250 bp to 10 000 bp:
100bp DNA ladder containing 13 discrete bands ranging from 100 bp to 3000 bp:
Loading Dyes
For loading and tracking DNA samples on gel:
