OZ Biosciences - NanOZ LNP-siRNA for Gene Silencing, Biodistribution and RNAi Kinetics
Ready-to-Use LNP-siRNA for Fluorescent Tracking and Functional Knockdown
NanOZ LNP-siRNA is a series of ready-to-use lipid nanoparticle formulations that protect siRNA from degradation and promote efficient cellular uptake. The portfolio enables researchers to monitor biodistribution by fluorescence (GFP or Cy5) and to quantify siRNA-mediated gene silencing via GAPDH mRNA knockdown. With standardized LNP-siRNA tools, it becomes easier to design, compare and interpret gene silencing and RNAi kinetics studies across multiple organs and administration routes.
NanOZ LNP-siRNA – Concept and Technology
NanOZ LNP-siRNA from OZ Biosciences combines siRNA with lipid nanoparticles to create a protected, transport-ready complex. The LNP shell shields siRNA from nuclease degradation while facilitating uptake into cells, thereby potentiating the RNAi effect. Using defined, ready-made formulations helps reduce variability, simplifies experimental setup and provides a robust basis for comparative biodistribution and gene silencing studies.
Key Advantages
siRNA Protection and Enhanced Uptake
Lipid nanoparticles protect siRNA cargo from enzymatic degradation and support efficient intracellular delivery. This improves the likelihood that the siRNA reaches the cytoplasm intact, leading to robust target gene silencing.
Ready-to-Use, Standardized LNP-siRNA Formats
NanOZ LNP-siRNA is supplied as ready-to-use formulations, reducing hands-on preparation time.
- Consistent particle properties between experiments
- Less variability from in-house formulation steps
- Faster transition from study design to execution
Fluorescent Biodistribution and Imaging (GFP, Cy5)
GFP and Cy5-labelled NanOZ LNP-siRNA formulations enable direct visualization of biodistribution.
- Track fluorescent signal in tissues and organs
- Compare uptake profiles between formulations and routes
- Align imaging readouts with functional RNAi data
Functional RNAi & Gene Silencing Assessment (GAPDH)
With GAPDH-targeting LNP-siRNA, NanOZ allows precise measurement of RNAi efficiency.
- Provides a reliable indicator of gene silencing potency
- Supports time-course analysis of RNAi kinetics
- Helps optimize dosing strategies and study design
Data using NanOZ LNP-siRNA
NanOZ LNP-siRNA allows efficient siRNA mediated gene silencing in cell lines
Gene silencing in various cell lines: GFP stably expressing cells were transfected with 5 nM siRNA against GFP encapsulated in LNP (LNP-siRNA GFP) or complexed with Lullaby (Lullaby) or 5 nM siRNA against GAPDH encapsulated in LNP (LNP-siRNA GAPDH). 72H after transfection, inhibition of fluorescence intensity was observed under fluorescence microscopy (A) and % of inhibition was calculated against Non Treated cells (NT cells) by flow cytometry (B).
NanOZ LNP efficiently deliver siRNA
SEAP Expression with HYPE-293 Kit in increasing volume of culture. Transfections were performed using 0.75 or 1.5 µg of plasmid DNA per mL of cell culture. Ratio of 2 µL of HYPE293 reagent per µg of plasmid was used. Density of cell culture was 1×106 cells/mL the day of transfection.
Product Portfolio: 3 Complementary LNP-siRNA
NanOZ LNP-siRNA GFP
Reporter Gene Silencing & Biodistribution
- siRNA targeting GFP for sequence-specific knockdown of green fluorescent protein
- Ideal to assess gene silencing efficiency and optimize delivery systems
- Transient, non-integrative, post-transcriptional knockdown – minimal genomic risk
- Fluorescent GFP signal allows monitoring of biodistribution and RNAi kinetics in various organs
- LNPs are suitable for parenteral administration routes
NanOZ LNP-siRNA scrambled Cy5
Non-Targeting Fluorescent Control
- Cy5-labelled scrambled siRNA for negative control in imaging and tracking experiments
- Visualizes cellular uptake, trafficking and organ biodistribution by fluorescence or confocal microscopy
- Scrambled sequence does not target endogenous mRNA → no specific gene silencing
- Distinguishes sequence-specific RNAi effects from non-specific responses to delivery or formulation
- LNPs are suitable for parenteral administration routes
NanOZ LNP-siRNA GAPDH
Functional Knockdown Control
- siRNA targeting GAPDH as a robust positive control for gene silencing
- Enables quantitative assessment of GAPDH mRNA knockdown to benchmark delivery platforms
- Transient, non-integrative, post-transcriptional suppression – low genomic risk
- Supports analysis of RNAi kinetics and functional readouts in multiple organs
- LNPs are suitable for parenteral administration routes