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Zymo Research -
High-Throughput Nucleic Acid Purification Solutions
Automation Kits for DNA, RNA and Plasmid DNA purification
Zymo Research provides a comprehensive range of products and solutions that are designed to handle a large number of samples efficiently and simultaneously. The Zymo Research high-throughput and automation portfolio include DNA, RNA and Plasmid DNA purification, Clean-up procedures, as well as high-throughput solutions in the fields of Microbiomics and Epigenetics.
Plasmid DNA - High-throughput purification kits
ZymoPURE™ 96 Plasmid Miniprep: highly concentrated, transfection-grade Plasmid DNA
Get transfection-grade Plasmid DNA suitable for any sensitive downstream applications.
ZymoPURE™ 96 Plasmid Miniprep Kit: Advantages
- Performs excellent with both, Vacuum Manifold or Centrifuge
- High DNA binding capacity (100µg) for greater plasmid DNA yields
- Low elution volume (125µL) for highly concentrated Plasmid DNA (up to 1µg/µl)
- Optimized wash regimen for plasmid DNA that is free of endotoxins, salt, protein, and RNA
- No cross-contamination thanks to unique high-throughput plates
- Zero buffer retention thanks to the unique vacuum plate design
- Colored buffers for error-free visualization of complete bacterial cell lysis and neutralization
- Filter Plates are included for rapid lysate clearing
ZymoPURE™ 96 Plasmid Miniprep Kit: Highest Yield and Concentration using a high-throughput method
Yield and Concentration for plasmid DNA using the ZymoPURE™ 96 Plasmid Miniprep kit compared to two popular 96 vacuum plate kits from Supplier Q and Supplier MN. Plasmid DNA isolated with the ZymoPURE™ 96 Plasmid Miniprep kit achieves a 5x increased yield and is 3x more concentrated.
ZymoPURE™ 96 Plasmid Miniprep Kit: Robust Purification of High-Quality Plasmid DNA
Novel 96-Well Wash Plate Significantly Reduces Cross-Contamination
Percent cross-contamination of two popular 96 vacuum plate kits from Supplier Q and Supplier MN compared to ZymoPURE™ 96 Plasmid Miniprep kit.
Eluted Plasmid DNA is Transfection-Ready
Transfection efficiency of plasmid prepared using ZymoPURE chemistry compared to two popular endotoxin-free kits from Supplier Q and Supplier MN.
Plasmid DNA high-throughput kits - Portfolio & Specifications
| Product | Cat# | Qty | Time | Culture Input | Min. Elution Vol. | Plasmid Yield | Endotoxins |
| ZymoPURE™ 96 Plasmid Miniprep Kit | D4214 D4215 |
2x 96 4x 96 |
≤ 60 min | 5 ml | ≥ 125 µl | up to 100 μg | ≤ 1.0 EU/µg |
| Zyppy-96 Plasmid Kit | D4041 D4042 D4043 |
2x 96 4x 96 8x 96 |
≤ 45min | 750µl | ≥ 30µl | up to 5μg | endotoxin-free (nd) |
| Zyppy-96 Plasmid MagBead Miniprep | D4100 D4101 D4102 |
2x 96 4x 96 8x 96 |
≤ 60min | 750µl | ≥ 30µl | up to 5μg | endotoxin-free (nd) |
Total DNA - Quick-DNA Kits
High-throughput isolation of total DNA (genomic, mitochondrial, viral) from any sample
The Quick-DNA Kits are the easiest DNA isolation kits for rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. Whole blood (fresh or stored), buffy coat, buccal cells, cells from culture, saliva, and other biological liquid samples can be processed with these DNA isolation kits. Eluted DNA from these DNA isolation kits is ideal for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
Quick-DNA™ MagBead Plus Kit
- Purify high-quality DNA easily and reliably from any biological fluid, cultured/monolayer cells, or tissue sample
- Automation friendly workflow enables any sample to be processed in as little as 60 minutes for 96 preps
- DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis
Compatible with Any Sample Type
Wide range of sample compatibility. DNA purified from different biological samples using the Quick-DNA™ MagBead Plus Kit is ultra-pure, highly concentrated, and ready for all downstream applications.
Superior Yields
Quick-DNA™ MagBead Plus Kit achieves superior yields. Replicate samples of 200 μl human whole blood were purified using Quick-DNA™ or a Supplier Q kit.
Quick-DNA™ Fecal/Soil Microbe Kits - DNA from Tough-to-Lyse Samples
- Rapid, high-throughput method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes
- Ultra-high density BashingBeads are fracture resistant and chemically inert
- Effectively removes PCR inhibitors from the DNA product
Ultra-Pure & PCR Inhibitor-Free
PCR inhibitor-free DNA with Quick-DNA™ Fecal/Soil Microbe Kit. Real-time PCR was used to evaluate 10% or 35% of eluates recovered using the Quick-DNA™ Fecal/Soil Microbe or Supplier A to detect PCR inhibitors. Delayed amplification indicates PCR inhibition from inefficient inhibitor removal (n=8).
Complete Homogenization
BashingBeads™ ensure complete homogenization. State-of-the-art BashingBeads™ are ideal for disrupting tough-to-lyse organisms when paired with bead mills or high speed cell disrupters.
DNA high-throughput kits - Portfolio & Specifications
| Product | Cat# | Qty | Binding Capacity | Min. Elution Vol. | Cells, Fluids | Tissue |
| Quick-DNA™ 96 Kit (no Proteinase K) | D3010 D3011 D3012 |
2x 96 4x 96 10x 96 |
5µg | 25µl | ≤ 106 | - |
| Quick-DNA™ 96 Plus Kit (Proteinase K included) | D4070 |
2x 96 4x 96 |
5µg | 15µl | ≤ 106 | ≤ 5mg |
| Quick-DNA™ Magbead Plus Kit | D4081 D4082 |
1x 96 4x 96 |
10µg | 100µl | ≤ 3 x 106 | ≤ 25mg |
| Quick-DNA Fecal/Soil Microbe 96 Kit | D6011 | 2x 96 | 5µg | 50µl | ≤ 80mg feces ≤ 135mg soil |
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| Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (Lysis Matrix Not Included) | D6011-FM | 2x 96 | 25µg | 37.5µl | - | ≤ 100mg feces ≤ 200mg soil |
| Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (includes ZR BashingBead™ Lysis Rack) | D6010-FM | |||||
| Quick-DNA Fecal/Soil Microbe 96 Magbead Kit (includes ZR BashingBead™ Lysis Tubes) | D6012-FM | |||||
| Quick-DNA™ Viral 96 Kit | D3017 D3018 |
2x 96 4x 96 |
5µg | 10µl | ≤ 200µl Fluids or ≤ 105 cells/ml | |
| Quick-DNA Fungal/Bacterial 96 Kit | D6006 | 2x 96 | 5µg | 25µl | 2x108 (bacterial) 2x107 (yeast or mammalian) |
≤ 20mg fungi or bacteria |
| Quick-DNA Tissue/Insect 96 Kit | D6017 | 2x 96 | 5µg | 25µl | ≤ 10mg tissue or insect | |
| Quick-DNA Plant/Seed 96 Kit | D6021 | 2x 96 | 5µg | 50µl | ≤ 80mg |
DNA and RNA Co-Extraction Kits - Quick-DNA/RNA Kits
Simultanoeus or sequential extraction of genomic DNA + total RNA (incl. small/micro RNA)
Discover easy, reliable, and rapid hifh-throughput DNA/RNA co-purification kits yielding high-quality total RNA, including small RNAs (17-200 nt), and genomic DNA without the use of phenol. These DNA/RNA co-extraction kits are fully compatible with downstream applications including reverse transcription, microarray, Next-Generation Sequencing, and more.
Quick-DNA/RNA MagBead Kit (96)
- Versatile: High-throughput, magnetic bead-based isolation of DNA and total RNA (including small/micro RNAs) from any sample including cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples), etc
- Quick & Easy: Isolate total nucleic acid or DNA and RNA in two separate fractions in < 15 minutes
- NGS-Ready: High-quality DNA & RNA is ready for any downstream application. DNase I included.
Co-Purification of Total Nucleic Acid
Co-Purification. High-quality total DNA and RNA was purified from human stool using the Quick-DNA/RNA™ MagBead kit. Elutions were analyzed in a 1% TAE/agarose/EtBr gel. The size marker is a 1kb ladder. input (n=2).
Consistently High Yields
High Yields. RNA yields are significantly higher when larger sample inputs are used with the Quick-DNA/RNA™ MagBead kit compared to a Supplier TF kit. 2.5*105 and 1*106 HeLa cells were used as input (n=2).
Quick-DNA/RNA™ Viral Kits
- Quick recovery of viral DNA/RNA from plasma, serum and other samples
- Omits the use of organic denaturants and proteases
- Streamlined Workflow: Sample inactivation and easy one-step lysis enables fast processing
- High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
Viral Inactivation
Viruses, bacteria, and yeast are effectively inactivated by DNA/RNA Shield™ (included in workflow) compared to mock (PBS) treatment for 5 minutes. Titer was subsequently determined by plaque assay (PFU) or growth assay (CFU).
High Sensitivity Viral Detection from Plasma
Sensitivity. Viral RNA isolated from plasma samples using the Quick-DNA/RNA™ Viral MagBead kit. Image shows average Cq values (in duplicate; +/- SD) from RT-PCR in comparison to Supplier A.
DNA/RNA high-throughput kits - Portfolio & Specifications
| Product | Cat.# | Qty | Binding Capacity | Min. Elution Vol. | Input |
| Quick-DNA/RNA™ MagBead Kit | R2130 R2131 |
1x 96 4x 96 |
15µg | 50µl | Cells, solid tissue, whole blood, biological liquids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples) |
| Quick-DNA/RNA Viral 96 Kit | D7022 D7023 |
2x 96 4x 96 |
5µg DNA + 10µg RNA | 10µl | Plasma/serum, swab, feces |
| Quick-DNA/RNA™ Viral MagBead Kit | R2140 R2141 |
1x 96 4x 96 |
5µg | 15µl | Plasma/serum, swab, feces |
| Quick-DNA/RNA™ Pathogen MagBead Kit | R2145 R2146 |
1x 96 4x 96 |
10µg | 50µl | Vectors, tissues |
RNA High-throughput Extraction Kits
Discover easy-to-use RNA extraction kits for total RNA purification including small RNAs, separate fractions of RNA, or RNA in the same sample. Produce high-quality, highly concentrated, DNA-free RNA that is suitable for downstream applications such as RT-PCR, hybridization, and sequencing. Explore RNA extraction kits based on sample types, formats, and downstream applications.
Direct-zol™ RNA - RNA from any sample in TRIzol / TRI-Reagent and similar
- Easy Handling: No phase separation, centrifugation, or precipitation steps
- NGS-Ready: Ultra-pure RNA without phenol carryover
- No DNA contamination (DNase I included)
- Non-Biased: Complete RNA recovery without miRNA loss
Efficient Small RNA Recovery
Small RNA recovery with the Direct-zol™ -96 MagBead RNA. Bioanalyzer® (Small RNA Chip) gel image shown.
Non-Biased miRNA Recovery
RNA purified from TRIzol® using Direct-zol™ RNA compared to an unbiased method (mirVana™, Ambion). Micro-RNA analysis was performed using miRNA-Seq (MiSeq®, Illumina) and a direct hybridization assay (nCounter®, Nanostring).
RNA high-throughput kits - Portfolio & Specifications
| Product | Cat.# | Qty | Binding Capacity | Min. Elution Vol. | Input |
| Direct-zol-96 RNA Kits | R2054 R2055 R2056 R2057 |
2x 96 2x 96 4x 96 4x96 |
10µg | 10µl | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Direct-zol-96 MagBead RNA | R2103 R2102 R2100 R2101 |
4x 96 4x 96 1x 96 1x96 |
10µg | 25µl | Any sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)). |
| Quick-RNA 96 Kit | R1052 R1053 |
2x 96 4x 96 |
10µg | 25µl | Cells or tissue samples, yeast, plant or bacteria |
| Quick-RNA MagBead | R2132 R2133 |
1x 96 4x 96 |
15µg | 30µl | Any cells, solid tissue, whole blood, biological fluids, FFPE tissue, environmental (plant/seed), swabs (stool, soil, microbial samples) |
| Quick-RNA Viral 96 Kit | R1040 R1041 |
2x 96 4x 96 |
10µg | 10µl | Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab and fecal samples |
Microbiome-grade DNA/RNA Purification - ZymoBIOMICS
ZymoBIOMICS® DNA kits
- Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, viruses, etc.
- Ultra-Pure: DNA/RNA is inhibitor-free and ready for qPCR and microbiome measurements using Next-Gen Sequencing
- High Sensitivity: Increased detection limit of very low abundance organisms. Certified low bioburden
Accurate Community Profiling
The ZymoBIOMICS® DNA kits provide accurate representation of the organisms extracted from the ZymoBIOMICS® Microbial Community Standard when compared with other extraction methods (Human Microbiome Project Protocol, Supplier M, Supplier Q).
Superior Yields
The ZymoBIOMICS® DNA Kits provides superior yields when compared to Suppliers M, P, and Q. 80 mg of feces was processed using each kit according to the manufactures’ recommended protocol. Elutions were analyzed in a 1.0% (w/v) TAE/ agarose/EtBR gel. L is a 1 kb ladder.
Microbiome-grade high-throughput kits - Portfolio & Specifications
| Product | Cat.# | Qty | Binding Capacity | Min. Elution Vol. | Input |
| ZymoBIOMICS MagBead RNA | R2137 R2138 |
1x 96 4x 96 |
15µg | 50µl | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| ZymoBIOMICS® 96 MagBead DNA/RNA Kit | R2135 R2136 |
1x 96 4x 96 |
15µg | 50µl | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50 mg of mammalian feces, ≤ 50 mg soil, and 5-20 mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs. |
| ZymoBIOMICS 96 DNA Kit (Lysis Rack) | D4303 | 2x 96 | 5µg | 20µl | Fecal ≤100 mg, Soil ≤ 100 mg, Cells ≤ 20 mg (Approximately equal to 2x108 bacterial, 2x107 yeast, or 2x106 mammalian cells) |
| ZymoBIOMICS 96 DNA Kit (No Lysis Matrix) | D4307 | ||||
| ZymoBIOMICS 96 DNA Kit (Lysis Tubes) | D4309 | ||||
| ZymoBIOMICS 96 MagBead DNA Kit (No Lysis Matrix) | D4302 | 2x 96 | 10µg | 37.5µl | Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA is efficiently isolated from feces, soil, fungal/bacterial cells, biofilms and water. |
| ZymoBIOMICS 96 MagBead DNA Kit (No Lysis Matrix) | D4306 | ||||
| ZymoBIOMICS 96 MagBead DNA Kit (Lysis Tubes) | D4308 |
Epigenetics High-Throughput/Automated Isolation
EZ DNA Methylation™ - High throughput kits for fast, easy automated bisulfite conversion
- Fast Bisulfite Conversion: Optimized bisulfite chemistry ensures improved conversion rate of DNA and reliable results
- Streamlined Workflow: Built-in desulphonation maximizes efficiency and reduces kit processing time
- Ready for Analysis: Ultra-pure DNA is ready for PCR, MSP, array, Next-Gen Sequencing, and other molecular assays.
High-Quality Intact DNA
EZ DNA Methylation-Lightning™ Kit yields more intact DNA after bisulfite conversion than the comparable kit from Supplier Q.
Improved Bisulfite Chemistry
Methylated DNA was converted using either conventional or EZ DNA MethylationDirect™ bisulfite chemistries. Direct bisulfite chemistry improves the rate and extent (> 99.8%) of C to U conversion of DNA as compared to conventional methods.
High-Quality Methylation Data
Methylation Plot From Reduced Representation Bisulfite Sequencing (RRBS). Data shows the relative methylation % at individual CpG sites in mouse DNA. Methylation percentage is shown across a ~3 Mb region of mouse chromosome 19.
Epigenetic high throughput kits - Portfolio & Specifications
| Product | Cat.# | Qty | Time | Recovery | Conversion | Min. Elution Vol. | Input |
| EZ-96 DNA Methylation Kit (Deep-Well) | D5004 | 2x 96 | 12-16 hours | >80% | >99% | 15µl | 500pg - 2µg of DNA |
| EZ-96 DNA Methylation Kit (Shallow-Well) | D5003 | 30µl | |||||
| EZ-96 DNA Methylation MagPrep | D5040 D5041 |
4x 96 8x 96 |
12-16 hours | >70% | >99% | 25µl | 500pg - 2µg of DNA |
| EZ-96 DNA Methylation-Gold Kit (Deep-well) | D5008 | 2x 96 | 3 hours | >75% | >99% | 15µl | 500pg - 2µg of DNA |
| EZ-96 DNA Methylation-Gold Kit (Shallow-well) | D5007 | 30µl | |||||
| EZ-96 DNA Methylation-Gold MagPrep | D5042 D5043 |
4x 96 8x 96 |
3 hours | >70% | >99% | 25µl | 500pg - 2µg of DNA |
| EZ-96 DNA Methylation-Direct Kit (Deep-well) | D5023 | 2x 96 | 4 hours | >80% | >99.5% | 15µl | 500pg - 2µg of DNA |
| EZ-96 DNA Methylation-Direct Kit (Shallow-well) | D5022 | 30µl | |||||
| EZ-96 DNA Methylation-Direct MagPrep | D5045 D5044 |
4x 96 8x 96 |
4 hours | >80% | >99.5% | 25µl | 500pg - 2µg of DNA |
| EZ-96 DNA Methylation-Lightning Kit (Deep-Well) | D5033 | 2x 96 | 1.5 hours | > 80% | >99.5% | 15µl | 100 pg - 2 µg of DNA. |
| EZ-96 DNA Methylation-Lightning Kit (Shallow-Well) | D5032 | 30µl | |||||
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D5046 D5047 |
4x 96 8x 96 |
1.5 hours | > 80% | >99.5% | 25µl | 100 pg - 2 µg of DNA. |
