30-years Zymo Research Promotion

Turbocharge Your Research with ZymoPURE for Transfection-Grade Plasmid DNA in under 20 minutes!

-35% on ZymoPURE Kits

Expires 30th June 2024. Not summable with other discounts.

Get a FREE vacuum manifold with order of ZymoPURE kits (> 500.- netto)

Free vacuum manifold (S7000) is only offered for ZymoPURE orders exceeding 500 CHF and only while stocks last. First come first served.

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Zymo Research - ZymoPURE Plasmid Purification
Simplify the Preparation of Transfection-Grade Plasmid DNA

ZymoPURE Endotoxin-Free Plasmid Purification Kits - Minipreps, Midipreps & Maxipreps

The ZymoPURE plasmid purification kits are the best method for easy and rapid isolation of transfection-grade plasmid DNA in minutes. Plasmid DNA purification using Minipreps, Midipreps and Maxipreps is performed in less than 18 minutes and Gigapreps in 45 minutes. Processing time is reduced by up to 9x using a vacuum manifold or centrifuge. Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization. 

Eluted DNA is free of endotoxins, salt, protein, and RNA, resulting in plasmids that are suitable for use in sensitive applications, including in vivo injections.

Fastest & Simplest Protocol

Highest Plasmid DNA Yields & Concentrations

Plasmid DNA is Endotoxin-Free

Plasmid DNA is Transfection-Grade

Vacuum manifold OR Centrifuge

High-throughput 96-well format available

Features of ZymoPURE (ZP) Plasmid DNA Kits

The ZymoPURE family of plasmid purification kit formats handle different amounts of culture input, ranging from miniprep (≤ 5 ml), midiprep (≤ 50 ml), maxiprep (≤ 150 ml) and gigaprep (≤ 2.5 L).

ZP II
Miniprep Kit
ZP 96
Miniprep Kit
ZP II
Midiprep Kit
ZP II
Maxiprep Kit
ZP II
Gigaprep Kit
ZP-Express
Midiprep Kit
Input
≤ 5ml
≤ 5ml
≤ 50ml
≤ 150ml
≤ 2.5L
≤ 25ml
Elution
≤ 25ul
≤ 125ul
≤ 150ul
≤ 300ul
≤ 3ml
≤ 150ul
Time
≤ 15 minutes
≤ 60 minutes
≤ 18 minutes
≤ 18 minutes
≤ 45 minutes
≤ 15 minutes
Yield
≤ 100 µg
≤ 100 µg
≤ 1.2 mg
≤ 3 mg
≤ 25 mg
≤ 1.2 mg
Endotoxin
≤ 1 EU/µg DNA
≤ 1 EU/µg DNA
≤ 0.025 EU/µg DNA
≤ 0.025 EU/µg DNA
≤ 0.025 EU/µg DNA
≤ 1 EU/µg DNA
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Fastest Plasmid DNA Purification Method - 16-minutes Midi/Maxi preps

Save more than 2 hours per Prep!

Zymopure - Bind, Wash, Elute
Zymopure - only 16 minutes
ZymoPURE low-high-throughput

Simply bind, wash and elute.

Novel binding chemistry and spin-column enables simple purification of transfection-ready plasmid DNA up to 9x faster using a vacuum manifold or centrifuge.

No slow gravity flow columns, no tedious alcohol precipitation.

Endotoxin-Free Plasmid Purification Reinvented - 16 minutes only with vacuum manifold protocol!

ZymoPURE Vacuum manifold

Highest yield and lowest elution volume

Zymopure - Highest yield lowest elution volume

Up to 3x More Plasmid DNA Yield

Zymopure - Yield competitor comparison

Up to 6x More Concentrated Plasmid

Zymopure - Concentration competitor comparison

Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free kits from Supplier Q and Supplier MN. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).

Transfection-grade Plasmid DNA

ZymoPURE Transfection-Ready

Endotoxin-Free

Purify Endotoxin-Free Plasmid with ZymoPURE II Midi, Maxi & Gigaprep Kits

Application-ready

CRISPR, gene modification, lentiviral vectors, synthetic biology, etc.

Ultra-Pure

For sensitive primary cells, in vivo injections, cloning, etc.

Eluted Plasmid DNA is Transfection-Ready

ZymoPURE Plasmid-grade comparison

Transfection efficiency of plasmid prepared using ZymoPURE chemistry compared to two popular endotoxin-free kits from Supplier Q and Supplier MN. HeLa cells seeded in a 96-well plate were transfected with 0.2 µg of pGL3® plasmid isolated from an overnight E. coli culture. Luciferase activity was measured after 48 hours.

Superior Transfection Efficiency

ZymoPure - Superior Transfection Efficiency

Data generated by V.B. at University of Cologne

HeLa Cells seeded in a 6-well plate were transfected with either 2 or 4 µg of pCI-neo®+GFP plasmid isolated from 100 ml of bacteria culture using the ZymoPURE Midiprep Kit, ZymoPURE Maxiprep Kit, or MN Midiprep Kit. GFP expressin was assessed 48 hours later in cell lysates using western blot and Ponceau S staining. The blot was also probed with an antibody against alpha-Tubulin in order to verify equal loading samples.

ZymoPURE's Unrivaled technology vs. Competitor Kits

Zymopure - Feature Comparison with Competitors

*Manufacturer’s stated maximum yield

**Defined as < 0.1 EU/µg of plasmid DNA

ZymoPURE plasmid purification kits are the fastest and simplest plasmid purification methods available to efficiently isolate a high yield of transfection-grade plasmid DNA from E. coli in as little as 16 minutes. Plasmid DNA is endotoxin-free and ready for immediate use in any downstream applications.

ZymoPURE Citations for Sensitive Applications

  1. Ramos-Murillo, Ana Isabel, et al. Efficient Non-Viral Gene Modification of Mesenchymal Stromal Cells from Umbilical Cord Wharton’s Jelly with Polyethylenimine. Pharmaceutics 12 9 896 2020.
  2. Vaughan, Hannah J, et al. Poly (beta-amino ester) nanoparticles enable tumor-specific TRAIL secretion and a bystander effect to treat liver cancer. Molecular Therapy-Oncolytics 21 377-388 2021.
  3. Lemmerman, Luke R, et al. Nanotransfection-based vasculogenic cell reprogramming drives functional recovery in a mouse model of ischemic stroke. Science Advances 712 eabd4735 2021.
  4. Gross, Tobias, et al. Characterization of CRISPR/Cas9 RANKL knockout mesenchymal stem cell clones based on single-cell printing technology and Emulsion Coupling assay as a low-cellularity workflow for single-cell cloning. Plos one 16 3 e0238330 2021.
  5. Tang, Shirley, et al. Nonviral Transfection With Brachyury Reprograms Human Intervertebral Disc Cells to a Pro‐Anabolic Anti‐Catabolic/ Inflammatory Phenotype: A Proof of Concept Study. Journal of Orthopaedic Research 37 11 2389-2400 2019.
  6. McMahon, Moira A, et al. Gene disruption using chemically modified CRISPR-Cpf1 RNA. CRISPR Guide RNA Design 49-60 2021.
  7. Zhang, Liyang, et al. AsCas12a ultra nuclease facilitates the rapid generation of therapeutic cell medicines. Nature Communications 12 1 44211 2021.
  8. Moore, Jordan T, et al. Nanochannel‐Based Poration Drives Benign and Effective Nonviral Gene Delivery to Peripheral Nerve Tissue. Advanced Biosystems 4 11 2000157 2020.
  9. Tang, S, et al. Non-viral reprogramming of human nucleus pulposus cells with FOXF1 via extracellular vesicle delivery: an in vitro and in vivo study. European Cells & Materials 41 90-107 2021.
  10. Chamberlain, Kyle, et al. A calsequestrin cis-regulatory motif coupled to a cardiac troponin T promoter improves cardiac adeno-associated virus serotype 9 transduction specificity. Human Gene Therapy 29 8 927-937 2018.
  11. Roy, Sashwati, et al. Neurogenic tissue nanotransfection in the management of cutaneous diabetic polyneuropathy. Nanomedicine: Nanotechnology, Biology and Medicine 2 102220 2020.
  12. Alzate-Correa, Diego, et al. Isolation and Nanoscale Electroporation of Primary Neuronal Cultures In Situ. Electroporation Protocols 145-152 2020.
  13. Li, Linxi, et al. Endogenously produced LG3/4/5-peptide protects testes against toxicant-induced injury. Cell Death & Disease 11 6 44215 2020.
  14. Yang, Xiao-Fei, et al. Direct reprogramming of hepatocytes into insulin-producing cells for anti-diabetic treatment by ultrasound-targeted microbubble destruction enhanced hydrodynamic gene delivery. American Journal of Translational Research 12 11 7275 2020.
  15. Rodriguez, Juan A, et al. Ghrelin receptor agonist rescues excess neonatal mortality in a Prader-Willi syndrome mouse model. Endocrinology 159 12 4006-4022 2018.
  16. Gurramkonda, Chandrasekhar, et al. Improving the recombinant human erythropoietin glycosylation using microsome supplementation in CHO cell‐free system. Biotechnology and Bioengineering 115 51253-1264 2018.
  17. Tran, Kevin, et al. Cell‐free production of a therapeutic protein: Expression, purification, and characterization of recombinant streptokinase using a CHO lysate. Biotechnology and Bioengineering 115 1 92-102 2018.
  18. Hunter, Dominic JB, et al. Unexpected instabilities explain batch‐to‐batch variability in cell‐free protein expression systems. Biotechnology and Bioengineering 11581904-1914 2018.
  19. Peñalber‐Johnstone, Chariz, et al. Optimizing cell‐free protein expression in CHO: Assessing small molecule mass transfer effects in various reactor configurations. Biotechnology and Bioengineering 114 7 1478-1486 2017.
  20. Keating, Sheila M, et al. Generation of recombinant hyperimmune globulins from diverse B-cell repertoires. Nature Biotechnology 44207 2021.
  21. Vazquez-Lombardi, Rodrigo, et al. Transient expression of human antibodies in mammalian cells. Nature Protocols 13 1 99-117 2018.
  22. Nguyen, Annalee W, et al. Engineering Antibodies on the Surface of CHO Cells. Genotype Phenotype Coupling 397-422 2020.
  23. Wang, Chensu, et al. Reprogramming NK Cells and Macrophages via Combined Antibody and Cytokine Therapy Primes Tumors for Elimination by Checkpoint Blockade. CellPress Ahead of Print 2021.
  24. Adler, Adam S, et al. Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomics. MAbs 9 8 1282-1296 2017.
  25. Vazquez-Lombardi, Rodrigo; et al. Expression of IgG Monoclonals with Engineered Immune Effector Functions. Antibody Engineering 313-334 2018.
  26. Piepenbrink, Michael S, et al. Therapeutic activity of an inhaled potent SARS-CoV-2 neutralizing human monoclonal antibody in hamsters. Cell Reports Medicine 2 3 100218 2021.
  27. Haabeth, Ole AW, et al. An mRNA SARS-CoV-2 vaccine employing Charge-Altering Releasable Transporters with a TLR-9 agonist induces neutralizing antibodies and T cell memory. ACS Central Science 2021.
  28. Colluru, Viswa Teja, et al. Mini-intronic plasmid vaccination elicits tolerant LAG3+ CD8+ T cells and inferior antitumor responses. Oncoimmunology 5 10 e1223002 2016.
  29. Whitacre, DC, et al. Designing a therapeutic hepatitis B vaccine to circumvent immune tolerance. Human Vaccines & Immunotherapeutics 16 2 251-268 2020.
  30. Kath, Jonas, et al. Fast, efficient and virus-free generation of TRAC-replaced CAR T cells. CellPress Ahead of Print 2021.
  31. Tsuji, Takemasa; et al. Rapid construction of antitumor T-cell receptor vectors from frozen tumors for engineered T-cell therapy. Cancer Immunology Research 6 5 594-604 2018.
  32. Shi, Xiaojing; et al. Genetically engineered cell-derived nanoparticles for targeted breast cancer immunotherapy. Molecular Therapy 28 2 536-547 2020.
  33. Duarte-Sanmiguel, Silvia, et al. Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells. Electroporation Protocols 79-84 2020.
  34. Cheng, Qinqin, et al. Reprogramming Exosomes for Immunotherapy. Cell Reprogramming for Immunotherapy 197-209 2020.
  35. Koblan, LW, et al., Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology 843-846 2018.
  36. Findlay, GM, et al. Accurate classification of BRCA1 variants with saturation genome editing. Nature 562 217-222 2018.
  37. Hu, JH, et al. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature 57-63 2018.
  38. Salasova, A, et al. A proteomic analysis of LRRK2 binding partners reveals interactions with multiple signaling components of the WNT/PCP pathway. Molecular Neurodegeneration 12 54 2017.
  39. Jaitin, D.A, et al. Dissecting immune circuits by linking CRISPR-Pooled Screens with Single-Cell RNA-Seq. Cell 1883-1896 2016.
  40. Marshall, R, et al. Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System. Molecular Cell 146-157 2017.
  41. Champer J, et al. Novel CRISPR/Cas9 gene drive constructs reveal insights into mechanisms of resistance allele formation and drive efficiency in genetically diverse populations. PLoS Genetics 13 7 2017.
  42. Aram, R, et al. Tools for Mos1-mediated single copy insertion (mosSCI) with excisable unc-119(+) or NeoR (G418) selection cassettes. microPublication Biology 2019.