Zymo Research Promotion
Get a FREE vacuum manifold with order of ZymoPURE kits (> 500.-)
Free vacuum manifold (S7000) is only offered for ZymoPURE orders exceeding 500 CHF and only while stocks last.
First come first served.
ZymoPURE Kits - Speed up your plasmid DNA purification
Minipreps, Midipreps and Maxipreps for Plasmid DNA Purification
The ZymoPURE plasmid purification kits are the best method for easy and rapid isolation of transfection ready plasmid DNA in minutes. Plasmid DNA purification using Minipreps, Midipreps and Maxipreps is performed in less than 18 minutes and Gigapreps in 45 minutes. Processing time is reduced by up to 9x using a vacuum manifold or centrifuge. Colored buffers permit error-free visualization and identification of complete bacterial cell lysis and neutralization.
Eluted DNA is free of endotoxins, salt, protein, and RNA, resulting in plasmids that are suitable for use in sensitive applications, including in vivo injections.
Comparison of ZymoPURE Plasmid DNA Purification Kits and their Features
ZymoPURE Plasmid Miniprep Kit | ZymoPURE II Plasmid Midiprep Kit | ZymoPURE II Plasmid Maxiprep Kit | ZymoPURE II Plasmid Gigaprep Kit | |
|---|---|---|---|---|
Volume | ≤ 5ml | ≤ 50ml | ≤ 150ml | ≤ 2.5L |
Time | ≤ 15 minutes | ≤ 18 minutes | ≤ 18 minutes | ≤ 45 minutes |
Yield | ≤ 100 µg | ≤ 1.2 mg | ≤ 3 mg | ≤ 25 mg |
Endotoxin | ≤ 1 EU/µg DNA | ≤ 0.025 EU/µg DNA | ≤ 0.025 EU/µg DNA | ≤ 0.025 EU/µg DNA |
Read more | Read more | Read more | Read more |
ZymoPURE is a fast Plasmid DNA Purification Method - Simply bind, wash, and elute
Save more than 2 hours per Prep!
Simply bind, wash and elute. Novel binding chemistry and spin-column enables simple purification of transfection-ready plasmid DNA up to 9x faster using a vacuum manifold or centrifuge. No slow gravity flow columns, no tedious alcohol precipitation.
ZymoPURE Plasmid DNA isolation provides the highest yield and lowest elution volume
Up to 3x More Plasmid DNA Yield
Up to 6x More Concentrated Plasmid
Yield and concentration for plasmid DNA isolated using the ZymoPURE II Maxiprep kit compared to two endotoxin-free kits from Supplier Q and Supplier MN. Plasmid DNA (pGL3®) was isolated from 150 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).
ZymoPURE provides Transfection-grade Plasmid DNA
Superior Transfection Efficiency
Data generated by V.B. at University of Cologne
Plasmid Purity Redefined
The EndoZero column eliminates residual endotoxins, yielding plasmid DNA with endotoxin levels below FDA limits for vaccines*. This means plasmid is suitable for sensitive applications like transfection of primary cells, in vivo injections, gene editing and recombinant viral vector production.
* Defined as endotoxins below the FDA limit for vaccines (< 0.04 EU/µg of plasmid DNA)
ZymoPURE Plasmid DNA Purification Features - Unrivaled technology
ZymoPURE plasmid purification kits are the fastest and simplest plasmid purification methods available to efficiently isolate a high yield of transfection-grade plasmid DNA from E. coli in as little as 16 minutes. Plasmid DNA is endotoxin-free and ready for immediate use in any downstream applications.
