IBA Lifesciences -
High-throughput applications using Strep-tag® technology

Strep-Tag® technology can be applied for easy, simple and cost-effective high-throughput protein purification and analysis.
No system switch necessary

  • For protein purification via affinity chromatography using Twin-Strep-tag® or Strep-tag®II fusion proteins, various sizes and formats of resins, spin columns and magnetic beads are available.
  • For protein analysis, high-affinity immobilization of Twin-Strep-tag® and Streptag®II proteins and ready-to-use microplates coated with Strep-Tactin®XT for assays are available.
  • For biomolecular interaction analysis IBA Lifesciences provides Bio-Layer Interferometry (BLI) or Surface Plasmon Resonance (SPR) Kits.

Strep-tag®: One affinity tag for protein purification and analysis

The Strep-tag® system enables cloning, expression, detection, purification, as well as further analysis of recombinant proteins. The highly specific interaction of the Strep-tag®II with Strep-Tactin® ensures efficient one-step purification of the protein of interest in unparalleled purity even from crude cell lysates.

StrepTag for Purification and Analysis

❯ Cost-effective: reusable reagents

❯ Time efficient: no system switch necessary

❯ Easy and simple procedures: one-step purification

Protein purification - High-throughput purifications

Application approach
Recommended product
Binding capacity
Required equipment
Spin columns
Up to 600 µg protein per column
Micropipettes, Centrifuge, Shaker
Magnetic beads in 96 well plate
Starting from 500 µg per well
Magnetic separator, Micropipettes, multi-channel pipettes or pipetting robot, Microplate shaker
Up to 50 µg per tip
Pipetting robot
High-throughput applications product formats

Manual applications

For small-scale experiments with a limited number of samples, spin columns can be used. Thereby the number of proteins that can be purified at the same time in a fast and efficient way is flexible. A Spin Column Kit with all necessary components for protein purification, as well as Empty Spin Columns that can be combined with the customer’s resin of choice are available.  


Strep-Tactin®XT 4Flow® high capacity Spin Column Kit for rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins

Strep-Tactin®XT 4Flow® suspensions and columns

Semi-automated applications

Magnetic beads, such as MagStrep “type3” XT beads, can be used in a round-bottom 96-well plate, allowing the simultaneous purification of up to 96 samples. The magnetic core of the beads allows the use of a magnet to fix the beads to the wells when removing the supernatant, diminishing the loss of beads and eliminating the need for centrifugation. 


MagStrep „type3“ XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins.

Automated applications

IMCSTips and Strep-TactinXT Scheme

For an automated approach that requires minimal manual intervention, IMCStips® containing Strep-Tactin®XT resin are available. The tip format allows high-throughput affinity purification with consistency and reproducibility making it especially attractive to industrial users. The approach is described in detail in the following application note

Read more about IMCStips

Protein analysis - High-throughput Screening Applications

The identified and purified proteins from the initial screening can then be further characterized by using the Strep-tag® technology: Due to the outstanding high affinity of the Twin-Strep-tag® to Strep-Tactin®XT (pM range), Strep-Tactin®XT is very well suited for assays based on ready-to-use microplates coated with Strep-Tactin®XT as well as for biomolecular interaction analysis technologies as Surface Plasmon Resonance (SPR) or Biolayer interferometry (BLI)

Strep-Tactin®XT Coated Microplate

Ready-to-use coated microplate

High-affinity immobilization of Twin-Strep-tag® and Streptag®II proteins. Ready-to-use!

Twin-Strep-tag® Capture Kit - SPR

Capture Kit

Reversible capture of Twin-Strep-tag® fusion proteins for Surface Plasmon Resonance (SPR) analysis

Strep-Tactin® XT BLI Coupling Kit

BLI Coupling Kit

Coating of BLI biosensors with Strep-Tactin®XT for reversible capture and analysis of Twin-Strep-tag® proteins.