IBA Lifesciences -
High-throughput applications using Strep-tag® technology
Strep-Tag® technology can be applied for easy, simple and cost-effective high-throughput protein purification and analysis.
No system switch necessary!
- For protein purification via affinity chromatography using Twin-Strep-tag® or Strep-tag®II fusion proteins, various sizes and formats of resins, spin columns and magnetic beads are available.
- For protein analysis, high-affinity immobilization of Twin-Strep-tag® and Streptag®II proteins and ready-to-use microplates coated with Strep-Tactin®XT for assays are available.
- For biomolecular interaction analysis IBA Lifesciences provides Bio-Layer Interferometry (BLI) or Surface Plasmon Resonance (SPR) Kits.
Strep-tag®: One affinity tag for protein purification and analysis
The Strep-tag® system enables cloning, expression, detection, purification, as well as further analysis of recombinant proteins. The highly specific interaction of the Strep-tag®II with Strep-Tactin® ensures efficient one-step purification of the protein of interest in unparalleled purity even from crude cell lysates.
❯ Cost-effective: reusable reagents
❯ Time efficient: no system switch necessary
❯ Easy and simple procedures: one-step purification
Protein purification - High-throughput purifications
Application approach | Recommended product | Binding capacity | Required equipment |
|---|---|---|---|
Manual | Spin columns | Up to 600 µg protein per column | Micropipettes, Centrifuge, Shaker |
Semi-automated | Magnetic beads in 96 well plate | Starting from 500 µg per well | Magnetic separator, Micropipettes, multi-channel pipettes or pipetting robot, Microplate shaker |
Automated | IMCStips® | Up to 50 µg per tip | Pipetting robot |
Manual applications
For small-scale experiments with a limited number of samples, spin columns can be used. Thereby the number of proteins that can be purified at the same time in a fast and efficient way is flexible. A Spin Column Kit with all necessary components for protein purification, as well as Empty Spin Columns that can be combined with the customer’s resin of choice are available.
Products:
Strep-Tactin®XT 4Flow® high capacity Spin Column Kit for rapid purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
Semi-automated applications
Magnetic beads, such as MagStrep “type3” XT beads, can be used in a round-bottom 96-well plate, allowing the simultaneous purification of up to 96 samples. The magnetic core of the beads allows the use of a magnet to fix the beads to the wells when removing the supernatant, diminishing the loss of beads and eliminating the need for centrifugation.
Products:
MagStrep „type3“ XT beads
Magnetic beads (5% suspension) to purify Strep-tag®II or Twin-Strep-tag® fusion proteins.
Automated applications
For an automated approach that requires minimal manual intervention, IMCStips® containing Strep-Tactin®XT resin are available. The tip format allows high-throughput affinity purification with consistency and reproducibility making it especially attractive to industrial users. The approach is described in detail in the following application note.
Protein analysis - High-throughput Screening Applications
The identified and purified proteins from the initial screening can then be further characterized by using the Strep-tag® technology: Due to the outstanding high affinity of the Twin-Strep-tag® to Strep-Tactin®XT (pM range), Strep-Tactin®XT is very well suited for assays based on ready-to-use microplates coated with Strep-Tactin®XT as well as for biomolecular interaction analysis technologies as Surface Plasmon Resonance (SPR) or Biolayer interferometry (BLI).
Strep-Tactin®XT Coated Microplate
High-affinity immobilization of Twin-Strep-tag® and Streptag®II proteins. Ready-to-use!
Twin-Strep-tag® Capture Kit - SPR
Reversible capture of Twin-Strep-tag® fusion proteins for Surface Plasmon Resonance (SPR) analysis
Strep-Tactin® XT BLI Coupling Kit
Coating of BLI biosensors with Strep-Tactin®XT for reversible capture and analysis of Twin-Strep-tag® proteins.
