IMCS - Automated high-throughput purifications in pipette tips

IMCStips | IMCSzyme - patented purification solutions for proteomics and genomics

IMCStips® is a patented, automated high-throughput dispersive solid-phase extraction (dSPE) technology used in proteomics and genomics. The loose resins contained in the pipette tips mix with sample solutions during aspirate and dispense cycles, ensuring maximum contact between the resin and your analytes of interest. This results in higher binding capacities and consistent, reproducible results in a short time.

IMCSzyme® is a collection of genetically modified pure beta-glucuronidase products with high efficiency. Consistent, stable and pure enzymes.

IMCStips is perfect for automating protein and nucleic acid purification

IMCStips® are the definitive choice when it comes to automated sample preparation. Designed with efficiency and user-friendliness in mind, this product uses patented dispersive solid-phase extraction (dSPE) to automate workflows while enhancing accuracy and consistency. 

IMCStips are compatible with a variety of automated liquid handling platforms, including Hamilton, Dynamic Devices, and INTEGRA VIAFLO 96. Purify and isolate samples in as little as 30 minutes!

IMCStips with Resin Procedure
  • Consistent, high recoveries 
  • Higher binding capacities 
  • Reproducible results in a short time
  • Flexible sample volumes
  • Customized applications
  • Streamlined, automated workflows for sample preparation, including protein purification, His-tag purification, nucleic acid purification, etc.
IMCStips Analyte Bound

Analyte binding per aspiration was assessed using bromophenol blue dye diluted in water at varying concentrations. The binding capacity of IMCStips is limited by concentration as well as the chemical property of the analyte of interest (i.e logP values).


Purification of a single log of CHO cell fermentation using MabSelect SuRe LX resin (50 μL resin bed) by centrifugation (spin) and IMCStips. For consistency, the spin method utilized 100 x g RCF for five minutes and flow through samples were re-applied to the column either 3x or 5x, similar to the tip format. Spin format consistency yielded less mAb than tip based format, as indicated by the UV-Vis measurements (Abs @ 280 nm) above.

Resins and Applications of IMCStips

Affinity Purification

Resin Options

  • Protein A, G, A/G
  • Ni-IMAC
  • Streptavidin
  • Strep-Tactin
  • Peptide-based


  • Antibody purification
  • Recombinant protein purification
  • Biotinylated protein purification
  • Purification of Strep-tagged proteins

Size Exclusion

Resin Options

  • SizeX100
  • SizeX150


  • Buffer exchange
  • Size exclusion chromatography
  • Automated multi-attribute method (MAM)


Resin Options

  • µPure LE
  • µPure LE Kit
  • µPure LE Kit with SizeX
  • IMCStips for PCR Cleanup


  • Nucleic acid purification or extraction
  • Low endotoxin plasmid purification
  • PCR cleanup or PCR product purification
  • NGS library prep or cleanup


Resin Options

  • PolyTi™
  • Zirconia


  • Automated phosphopeptide enrichment

Ion Exchange

Resin Options

  • µSAX
  • Macro SCX
  • Macro SAX
  • Macro WAX
  • Macro WCX


  • Ion exchange chromatography
  • µPure LE: Automated low endotoxin plasmid purification

Reverse Phase

Resin Options

  • P
  • Large Pore C4
  • Silica C8
  • Silica C18
  • OligoR


  • Peptide desalting
  • Protein desalting
  • DMT-on oligonucleotide purification

Automated Purification of IgG Antibodies using Protein A IMCStips®

IMCStips vs SpinColumn1
IMCStips vs SpinColumn2

Purification of a single lot of CHO cell fermentation using Protein A resin (50 µL resin bed) by centrifugation (spin) and IMCStips. For consistency, the spin method utilized 100 ×g for five minutes and flow through samples were re-applied to the column either 3x or 5x, similar to the tip format. The spin columns yielded less IgG than IMCStips, as indicated by both UV-Vis measurements and by SDS-PAGE.

Automated Affinity Purification and Buffer Exchange with IMCStips®

Affinity data A

(A) Histidine labeled proteins (GFP, PaS, GusA) were added into bacterial lysates and purified with 15 µL resin Ni-IMAC IMCStips followed by buffer exchange with SizeX IMCStips.

Affinity data B

(B) The recoveries for the Ni-IMAC IMCStips was optimal for 100 and 200 µg protein loads due to the 15 µL resin bed. More resin will assist with higher recoveries for larger quantities (400 and 600 µg).

Affinity data C

(C) SDS PAGE of the proteins at different quantities match the Bradford quantifications (see graphs in A).

Affinity data D

(D) Purified PaS are active (red wells) after undergoing affinity purification followed by buffer exchange on the liquid handler, and there is no detectable carryover between wells or tips.