Navinci - NaveniFlex™ Cell PLA

Proximity Ligation Assay (PLA) optimized for cell samples

The NaveniFlex™ Cell PLA is an in situ proximity ligation assay kit optimized for cell samples (FFPE and fresh formaldehyde-fixated cells). It enables in situ fluorescence visualization of protein-protein interactions or post-translational modifications such as phosphorylation and protein expressions. It allows to analyze even the most low-abundance protein targets with precision and ensures high specificity. The obtained data is quantifiable. High throughput screening with 96 well plate protocol is possible.

NOTE: NaveniFlex™ Cell represents an advanced refinement of the discontinued NaveniFlex. For tissue samples please consider using NaveniFlex™ Tissue PLA

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NaveniFlex™Cell - laboratory workflow and sample types

Workflow

Note: Shorter protocol compared to the workflow of the previous (discontinued) version (NaveniFlex Kits).

Sample types

NaveniFlex™Cell Applications

Detection of proteins, protein – protein interactions, or protein modifications in cell samples. Validated on FFPE and fresh formaldehyde-fixated cells (MCF7 and BT474), and recommended for FFPE and fresh formaldehyde-fixated cells.

Understanding biology, underlying pathophysiology and processes from health to disease
Signaling pathways
Cell and protein metabolism
Protein-protein interactions in cells

Biomarker studies in cells
Deep phenotypic profiling -> find better drug targets earlier
Biomarkers for disease or diagnosis
Drug target engagements

NaveniFlex™Cell Portfolio

Choose from six assay versions based on the primary IgG host species, mouse-rabbit (MR), goat-rabbit (GR) and goat-mouse (GM), and your preferred detection reagents from the fluorophore alternatives TEX615 (Red) and Atto647N.

NaveniFlex Cell MR Red

To be used with a mouse and rabbit primary antibody pair. Detection with TEX615 fluorophore is included. Details

NaveniFlex Cell GR Red

To be used with a goat and rabbit primary antibody pair. Detection with TEX615 fluorophore is included. Details

NaveniFlex Cell GM Red

To be used with a goat and mouse primary antibody pair. Detection with TEX615 fluorophore is included. Details

NaveniFlex Cell MR Atto647N

To be used with a mouse and rabbit primary antibody pair. Detection with Atto647N fluorophore is included. Details

NaveniFlex Cell GR Atto647N

To be used with a goat and rabbit primary antibody pair. Detection with Atto647N fluorophore is included.

NaveniFlex Cell GM Atto647N

To be used with a goat and mouse primary antibody pair. Detection with Atto647N fluorophore is included.

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