Reinventing Immunostaining and
in situ proximity ligation
The Naveni technology offers the possibility to study proteins and their interactions in situ at the molecular level, while maintaining the spatial resolution and integrity of the tissue microenvironment.
Navinci’s researchers are the developpers of the Duolink® technology. With the Naveni technology, they bring the next generation technology to the market.
Assay principle
After the incubation with the primary antibody, the sample is incubated with the Navenibodies which function as a secondary antibody. If the Navenibody pairs are in close proximity, they become activated (digestion) and the circle creation is initiated. Once completed the signal is amplified by rolling circle amplification. The resulting fluorescent signal can be read out using a fluorescent microscope and quanitified with most image analysis softwares.
Comparison with Duolink®
Key Advantages
10x increase in sensitivity compared to traditional PLA
NaveniFlex Kits feature a new proprietary probe design technology providing advantages compared to traditional Duolink® PLA:
- Enables detection of very low abundant proteins and interactions
- Only 1/10 of the antibody concentration required
- Significantly improved signal to noise ratio facilitates quantification
Increased specificity by dual recognition
If a suitable antibody pair is available, the Navinci technology can be used to achieve supreme specificity without background.
Example
Comparison of ACE2 detection by conventional IF and using Navenibodies. Upper images: Kidney mRNA: 100TPM; Lower images: Lung mRNA: 1.8 TPM
Signal Enhancement
The Naveni system features a significantly increased signal compared to conventional IF. Simply replace secondary antibodies with Navenibodies from NaveniFlex MM and RR kits for better results.
Phosphorylation Detection
The Navinci solution allows the detection of posttranslational modifications with unparralleled specificity and no background.
Product Guide
Which NaveniFlex Kit should I choose?
NaveniFlex kits contain Navenibodies (secondary detection antibodies conjugated to proprietary oligo arms) for use with either one or two 1° IgG antibodies raised in mouse (M), rabbit (R) or goat (G). Three fluorophore alternatives are included.
One 1° Ab + NaveniFlex MM or RR
- Signal enhancement of low abundant targets
- In case of insufficient sensitivity
Two 1° Ab + NaveniFlex MR, GR or GM
- in case of unspecific binding
- increased specificity in protein localization
- investigation of protein interactions
- investigation of protein modifications
NaveniFlex MM
Detects single mouse primary antibodies
NaveniFlex RR
Detects single rabbit primary antibodies
NaveniFlex MR, GM, GR
Detects proximity between a combination of
mouse / rabbit / goat pairs of primary antibodies
Naveni pTyr
Target specific assays to detect a variety of phosphorylated proteins
