Navinci - Proximity ligation assay (PLA) Portfolio

Reinventing Immunostaining

The Naveni technology offers the possibility to study proteins and their interactions in situ at the molecular level, while maintaining the spatial resolution and integrity of the tissue microenvironment.

Navinci’s researchers are the developpers of the Duolink® technology. With the Naveni technology, they bring the next generation technology to the market.

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PLA staining

Assay principle

Navinci NaveniFlex Workflow of PLA

After the incubation with the primary antibody, the sample is incubated with the Navenibodies which function as a secondary antibody. If the Navenibody pairs are in close proximity, they become activated (digestion) and the circle creation is initiated. Once completed the signal is amplified by rolling circle amplification. The resulting fluorescent signal can be read out using a fluorescent microscope.

Key Advantages

A. 10x increased sensitivity compared to traditional PLA

NaveniFlex Kits feature a new proprietary probe design technology providing advantages compared to traditional Duolink® PLA:

  • Enables detection of very low abundant proteins and interactions
  • Only 1/10 of the antibody concentration required
  • Significantly improved signal to noise ratio facilitates quantification

B. Signal Enhancement

The Naveni system features a significantly increased signal compared to conventional IF. Simply replace secondary antibodies with Navenibodies from NaveniFlex MM and RR kits for better results.

C. Increased specificity by dual recognition

If a suitable antibody pair is available, the Navinci technology can be used to achieve supreme specificity without background.

Comparison of ACE2 detection by conventional IF and using Navenibodies. Upper images: Kidney mRNA: 100TPM; Lower images: Lung mRNA: 1.8 TPM

D. Phosphorylation Detection

The Navinci solution allows the detection of posttranslational modifications with unparralleled specificity and no background.

Product Guide

NaveniFlex™ – Flexible PLA Kits

  • All necessary reagents included
  • Designed to offer flexibility in target selection and experimental design
  • Works with traditionalin situequipment and workflows
  • Choose the kit to match the primary antibodies

NaveniFlex™Cell – Optimized for cells

NaveniFlex Cell
NaveniFlex Cell

Study protein-protein interactions and protein interplay in cell samples. Analyze even the most low-abundance protein targets with precision. High throughput screening with 96 well plate protocol possible.

Read more | Details on supplier page

NaveniFlex™ Tissue – Optimized for tissues

NaveniFlex Tissue Example2
NaveniFlex Tissue Example1

In situ proximity ligation assay kit optimized for tissue samples. for a combination of mouse / rabbit / goat pairs of primary antibodies. Detection fluorophores: Atto 647N or Tex615 Red

Naveni™TriFlex Cell – Study protein interplay

Naveni TriFlex Cell Reaction

Detect and quantify total protein A and  B, and the interaction between AB. To be used with a mouse & rabbit primary antibody pair. Works with cells, cell pellets and cell cytospin.

NaveniBright™ - For chromogenic readout

Naveni Bright AP and HRP

Chromogenic detection of proteins, protein–protein interactions, or protein modifications in cultured cells, in fresh-frozen or FFPE samples. To be used with a mouse & rabbit primary antibody pair.

NaveniFlex™ Control kit

Control-kit

The NaveniFlex control kit has been designed as a control for the NaveniFlex kits proximity ligation reaction and to help the user distinguish between positive and negative proximity ligation signals.

NaveniLink™ - Expanding Your Options

NaveniLink Procedure

Create Your Own Navenibodies: Tailor your experiments to your specific requirements. Simplified conjugation featuring streamlined process and minimal hands-on time.

Target specific PLA Kits

  • Primary antibody system included
  • No extra antibodies are needed; the assay is already optimized
  • For detection of protein protein interactions in cultured cells, fresh-frozen, and FFPE samples

Naveni PTM - Post Translational Modifications

Navinci pTyr

Naveni pY EGFR, PDGFR-b, HER2, MET, VEGFR2, PD1

Target specific assays to detect and quantify Post Translational Modifications, such as phosphorylated proteins, in cultured cells, fresh-frozen and FFPE samples. The primary antibody system is included and optimized!

Naveni PPI - Protein-Protein Interaction

NaveniFlex RR

Naveni PD1/PD-L1 AP, HRP or Atto 647N

Specific and optimized in situ kits for detection of protein protein interactions in cultured cells, fresh-frozen, and FFPE samples. The primary antibody system is included and optimized!