Navinci Promotions
-25% Naveni™ TriFlex Cell
Study protein functional states and interplay in cells, cell pellets or cytospins
Offer expires 31st March 2023. Not summable with other discounts.
Navinci - Reinventing Immunostaining and in situ proximity ligation
The Naveni technology offers the possibility to study proteins and their interactions in situ at the molecular level, while maintaining the spatial resolution and integrity of the tissue microenvironment.
Navinci’s researchers are the developpers of the Duolink® technology. With the Naveni technology, they bring the next generation technology to the market.
Assay principle
After the incubation with the primary antibody, the sample is incubated with the Navenibodies which function as a secondary antibody. If the Navenibody pairs are in close proximity, they become activated (digestion) and the circle creation is initiated. Once completed the signal is amplified by rolling circle amplification. The resulting fluorescent signal can be read out using a fluorescent microscope and quanitified with most image analysis softwares.
Comparison with Duolink®
Key Advantages
10x increase in sensitivity compared to traditional PLA
NaveniFlex Kits feature a new proprietary probe design technology providing advantages compared to traditional Duolink® PLA:
- Enables detection of very low abundant proteins and interactions
- Only 1/10 of the antibody concentration required
- Significantly improved signal to noise ratio facilitates quantification
Increased specificity by dual recognition
If a suitable antibody pair is available, the Navinci technology can be used to achieve supreme specificity without background.
Example
Comparison of ACE2 detection by conventional IF and using Navenibodies. Upper images: Kidney mRNA: 100TPM; Lower images: Lung mRNA: 1.8 TPM
Signal Enhancement
The Naveni system features a significantly increased signal compared to conventional IF. Simply replace secondary antibodies with Navenibodies from NaveniFlex MM and RR kits for better results.
Phosphorylation Detection
The Navinci solution allows the detection of posttranslational modifications with unparralleled specificity and no background.
Product Guide
Which NaveniFlex Kit should I choose?
NaveniFlex kits contain Navenibodies (secondary detection antibodies conjugated to proprietary oligo arms) for use with either one or two 1° IgG antibodies raised in mouse (M), rabbit (R) or goat (G). Three fluorophore alternatives are included.
One 1° Ab + NaveniFlex MM or RR
- Signal enhancement of low abundant targets
- In case of insufficient sensitivity
Two 1° Ab + NaveniFlex MR, GR or GM
- in case of unspecific binding
- increased specificity in protein localization
- investigation of protein interactions
- investigation of protein modifications
Flexible PLA Kits
NaveniBright AP or HRP
Chromogenic detection of proteins, protein–protein interactions, or protein modifications in cultured cells, in fresh-frozen or FFPE samples. To be used with a mouse & rabbit primary antibody pair.
Naveni TriFlex Cell MR
Detect and quantify total protein A and B, and the interaction between AB. To be used with a mouse & rabbit primary antibody pair. Works with cells, cell pellets and cell cytospin.
NaveniFlex Tissue GM, GR or MR
In situ proximity ligation assay kit optimized for tissue samples. for a combination of mouse / rabbit / goat pairs of primary antibodies. Detection fluorophores: Atto 647N or Tex615 Red
Target specific PLA Kits
Naveni Post Translational Modifications (PTM)
Naveni pY
EGFR, PDGFR-b, HER2, MET, VEGFR2, PD1
Target specific assays to detect and quantify Post Translational Modifications, such as phosphorylated proteins, in cultured cells, fresh-frozen and FFPE samples. The primary antibody system is included and optimized!
Naveni Protein-Protein Interaction (PPI)
Naveni PD1/PD-L1
AP or HRP
Specific and optimized in situ kits for detection of protein protein interactions in cultured cells, fresh-frozen, and FFPE samples. The primary antibody system is included and optimized!
