Navinci - Proximity ligation assay (PLA) Portfolio
Reinventing Immunostaining
The Naveni technology offers the possibility to study proteins and their interactions in situ at the molecular level, while maintaining the spatial resolution and integrity of the tissue microenvironment.
Navinci’s researchers are the developpers of the Duolink® technology. With the Naveni technology, they bring the next generation technology to the market.
Assay principle
After the incubation with the primary antibody, the sample is incubated with the Navenibodies which function as a secondary antibody. If the Navenibody pairs are in close proximity, they become activated (digestion) and the circle creation is initiated. Once completed the signal is amplified by rolling circle amplification. The resulting fluorescent signal can be read out using a fluorescent microscope.
Key Advantages
A. 10x increased sensitivity compared to traditional PLA
NaveniFlex Kits feature a new proprietary probe design technology providing advantages compared to traditional Duolink® PLA:
- Enables detection of very low abundant proteins and interactions
- Only 1/10 of the antibody concentration required
- Significantly improved signal to noise ratio facilitates quantification
B. Signal Enhancement
The Naveni system features a significantly increased signal compared to conventional IF. Simply replace secondary antibodies with Navenibodies from NaveniFlex MM and RR kits for better results.
C. Increased specificity by dual recognition
If a suitable antibody pair is available, the Navinci technology can be used to achieve supreme specificity without background.
Comparison of ACE2 detection by conventional IF and using Navenibodies. Upper images: Kidney mRNA: 100TPM; Lower images: Lung mRNA: 1.8 TPM
D. Phosphorylation Detection
The Navinci solution allows the detection of posttranslational modifications with unparralleled specificity and no background.
Product Guide
NaveniFlex™ – Flexible PLA Kits
- All necessary reagents included
- Designed to offer flexibility in target selection and experimental design
- Works with traditionalin situequipment and workflows
- Choose the kit to match the primary antibodies
NaveniFlex™Cell – Optimized for cells
Study protein-protein interactions and protein interplay in cell samples. Analyze even the most low-abundance protein targets with precision. High throughput screening with 96 well plate protocol possible.
Read more | Details on supplier page
NaveniFlex™ Tissue – Optimized for tissues
In situ proximity ligation assay kit optimized for tissue samples. for a combination of mouse / rabbit / goat pairs of primary antibodies. Detection fluorophores: Atto 647N or Tex615 Red
Naveni™TriFlex Cell – Study protein interplay
Detect and quantify total protein A and B, and the interaction between AB. To be used with a mouse & rabbit primary antibody pair. Works with cells, cell pellets and cell cytospin.
NaveniBright™ - For chromogenic readout
Chromogenic detection of proteins, protein–protein interactions, or protein modifications in cultured cells, in fresh-frozen or FFPE samples. To be used with a mouse & rabbit primary antibody pair.
NaveniFlex™ Control kit
The NaveniFlex control kit has been designed as a control for the NaveniFlex kits proximity ligation reaction and to help the user distinguish between positive and negative proximity ligation signals.
NaveniLink™ - Expanding Your Options
Create Your Own Navenibodies: Tailor your experiments to your specific requirements. Simplified conjugation featuring streamlined process and minimal hands-on time.
Target specific PLA Kits
- Primary antibody system included
- No extra antibodies are needed; the assay is already optimized
- For detection of protein protein interactions in cultured cells, fresh-frozen, and FFPE samples
Naveni PPI - Protein-Protein Interaction
Naveni PD1/PD-L1 and CD8/MHC-I
Specific and optimized in situ kits for detection of protein protein interactions in cultured cells, fresh-frozen, and FFPE samples. The primary antibody system is included and optimized!
Naveni PTM - Post Translational Modifications
Naveni pY EGFR, PDGFR-b, HER2, MET, VEGFR2, PD1
Target specific assays to detect and quantify Post Translational Modifications, such as phosphorylated proteins, in cultured cells, fresh-frozen and FFPE samples. The primary antibody system is included and optimized!
